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The map label for this gene is pyrE

Identifier: 21226244

GI number: 21226244

Start: 177146

End: 177709

Strand: Direct

Name: pyrE

Synonym: MM_0142

Alternate gene names: 21226244

Gene position: 177146-177709 (Clockwise)

Preceding gene: 21226243

Following gene: 21226246

Centisome position: 4.32

GC content: 47.52

Gene sequence:

>564_bases
ATGAGCAAAACAAATTCAGAAACAGGAAATAATTTTGAACAACAGAAACAGGAACTTATCGCAGCCCTGAAAGCCTGCGG
AGCTGTCCGTTACGGGGACTTCACACTTGCTTCGGGAAAGAAAAGCAAGTATTATATAGACATCAAGAAAGCCAGCACGG
ACCCGAAAACCCTGAAAATAATTGCCAGGCAGGCAGCCCTCAGGATAAAAGAAATGGATGTGGATACAGTTGCAGGTGTG
GAACTCGGAGGAGTCCCTCTGGCAACCGCTGTTTCCCTGGAGACTGAACTCCCACTTCTGATTGTGAGAAAATCCGTCAA
GGACTACGGCACAAAAAGCAGGTTTGTTGGAGACCTGAAACCCGAGGACAGGCTTGTAATGCTTGAAGACGTAACAACCA
GCGGGGGCTCGGTGAGAGATGCGATCGAGGTTGTCAGAGAAGCAGGAGCCAGTTTAAAATACGTTATCACAGTAGTGGAC
AGAGAAGAAGGGGCAGGGGAAAAACTGAAAGAAGCAGATGTCGAACTTGTGCCTCTTGTAAGTGCAAGCGACCTGTTGAA
ATAA

Upstream 100 bases:

>100_bases
AACCGTGGTGAAAATTTCCGGGGAATTTAATAATATCCTTACAGGAAAAATCTAAGAAAACACATATAGCATTAATAAGG
CTGCAAATACCGGTGAGAAA

Downstream 100 bases:

>100_bases
AGAAAGTGCAGTAGCACTTTCCTGAATCTCTATTTAATAAAATTAGGATCCTTATAAATTGGAATAATAATGACAGTAAT
AATATTGTAATATTGTAATA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSKTNSETGNNFEQQKQELIAALKACGAVRYGDFTLASGKKSKYYIDIKKASTDPKTLKIIARQAALRIKEMDVDTVAGV
ELGGVPLATAVSLETELPLLIVRKSVKDYGTKSRFVGDLKPEDRLVMLEDVTTSGGSVRDAIEVVREAGASLKYVITVVD
REEGAGEKLKEADVELVPLVSASDLLK

Sequences:

>Translated_187_residues
MSKTNSETGNNFEQQKQELIAALKACGAVRYGDFTLASGKKSKYYIDIKKASTDPKTLKIIARQAALRIKEMDVDTVAGV
ELGGVPLATAVSLETELPLLIVRKSVKDYGTKSRFVGDLKPEDRLVMLEDVTTSGGSVRDAIEVVREAGASLKYVITVVD
REEGAGEKLKEADVELVPLVSASDLLK
>Mature_186_residues
SKTNSETGNNFEQQKQELIAALKACGAVRYGDFTLASGKKSKYYIDIKKASTDPKTLKIIARQAALRIKEMDVDTVAGVE
LGGVPLATAVSLETELPLLIVRKSVKDYGTKSRFVGDLKPEDRLVMLEDVTTSGGSVRDAIEVVREAGASLKYVITVVDR
EEGAGEKLKEADVELVPLVSASDLLK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=172, Percent_Identity=35.4651162790698, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI1790073, Length=148, Percent_Identity=33.1081081081081, Blast_Score=75, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=174, Percent_Identity=33.9080459770115, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508631, Length=174, Percent_Identity=31.6091954022989, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323530, Length=162, Percent_Identity=29.0123456790123, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323927, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17933654, Length=162, Percent_Identity=31.4814814814815, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METMA (Q8Q0J4)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632166.1
- ProteinModelPortal:   Q8Q0J4
- SMR:   Q8Q0J4
- GeneID:   1478484
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0142
- NMPDR:   fig|192952.1.peg.142
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- ProtClustDB:   PRK13810
- BioCyc:   MMAZ192952:MM0142-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 20203; Mature: 20072

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 103-103 BINDING 104-104 BINDING 107-107 BINDING 133-133 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTNSETGNNFEQQKQELIAALKACGAVRYGDFTLASGKKSKYYIDIKKASTDPKTLKI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEECCCEEEECCCCCEEEEEEEECCCCCHHHHH
IARQAALRIKEMDVDTVAGVELGGVPLATAVSLETELPLLIVRKSVKDYGTKSRFVGDLK
HHHHHHHHHHHCCCCHHCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHEECCCC
PEDRLVMLEDVTTSGGSVRDAIEVVREAGASLKYVITVVDREEGAGEKLKEADVELVPLV
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCEEEEEE
SASDLLK
CHHHHCC
>Mature Secondary Structure 
SKTNSETGNNFEQQKQELIAALKACGAVRYGDFTLASGKKSKYYIDIKKASTDPKTLKI
CCCCCCCCCCHHHHHHHHHHHHHHHCCEECCCEEEECCCCCEEEEEEEECCCCCHHHHH
IARQAALRIKEMDVDTVAGVELGGVPLATAVSLETELPLLIVRKSVKDYGTKSRFVGDLK
HHHHHHHHHHHCCCCHHCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHEECCCC
PEDRLVMLEDVTTSGGSVRDAIEVVREAGASLKYVITVVDREEGAGEKLKEADVELVPLV
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCEEEEEE
SASDLLK
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824