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The map label for this gene is FrvX [C]

Identifier: 20806950

GI number: 20806950

Start: 451831

End: 452871

Strand: Direct

Name: FrvX [C]

Synonym: TTE0441

Alternate gene names: 20806950

Gene position: 451831-452871 (Clockwise)

Preceding gene: 20806949

Following gene: 20806951

Centisome position: 16.8

GC content: 40.73

Gene sequence:

>1041_bases
ATGGATTATAAGGAGCTGTTAAAAAAGCTTAGTGAAAGTCACGGGGTCTCCGGACATGAAAGAGGAATTTATCAGCTTTT
GAAAAAAGAATTTGAAGAGATTTCCGATGAAGTTCTAGAGGACAATTTTGGAAATTTAATTTTTAAAAAGAAGGGTTTAA
AAGGCAAGTATAAGGTGATGCTGGCAGCCCATTTGGATGAAATAGGGCTTATGGTGAAAGATATTGATGAGAAAGGATTT
ATAAAATTTACACCGGTTGGAGGAGTGGACCAAAGAACCCTTCCTTCTCAAGAAGTCATAGTGCACGGTAAAAAAGAATT
ATTGGGAGTTATAGGGAGTAAACCGCCTCATCTCTTATCCTCCGAAGATATGGAGAAGGCAATAAAAATAGACGATATGT
ACGTAGACGTAGGGCTTCCGAAAGAGGAAGTAGAGAAGCTGGTAAGCATTGGAGATATTATAACTGTGAAAAGGGAGTTT
AGAGAACTTTTGAATGATAATGTTTCAGGAAAGGCTTTAGACGATAGGGCAGGGGTTGTGGTGATGGCAGTATGCCTTGA
TGAACTAAGGAAGATGTACCATTATCACGACGTGTATGCTGTAGCTACTCTTCAGGAAGAGGTGGGGGTAAGAGGAGCTA
TAACTTCTTCTTACAATATTGAACCTGACATAGCTATAGCGATTGATGTGACTCATGCGAAAGCGAGGGGAGTTAGCCGC
GACATAGAGATAGGGAAAGGGCCTGCGATTGGGAAGGGGCCTAATATTCATCCTGCTGTATATAAAGGGCTTGTAGATAT
AGCGAAAAAATACAATATAAATTATCAGATAGAGCCGCTTCCTGGGCATTCAGGCACTGATGCATGGGCAATCCAGGTGT
CAAAAAAAGGAGTTCCTACAGGGCTTGTGTCAATACCTTTGAAGTACATGCATACTTCTGTGGAAACTGCTAATATGAAA
GACATAATAGAAAGCGGCAGACTGCTGGCACACTATATTGCCAATCTGCCTGAGGAATTGGAGGGACATTTATGTTATTA
A

Upstream 100 bases:

>100_bases
GAAGGATTTTAGAGATTTATGTAGAAATAATAAACTCAGGAAGGGCAAAAGCAGAATTTTACCACTTTGTTTAATAAAAT
TAGATGGGAAGGGGTAGAAA

Downstream 100 bases:

>100_bases
AGGAGTTAACTGAGCTTTTAGGTGCTTCTGGAGATGAAAAAGAGGTAAGAGAGAAGATAAAAGAGATAGTTAAACCTTAT
GTGGATGAGCTTTATGTGGA

Product: cellulase M-like protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDYKELLKKLSESHGVSGHERGIYQLLKKEFEEISDEVLEDNFGNLIFKKKGLKGKYKVMLAAHLDEIGLMVKDIDEKGF
IKFTPVGGVDQRTLPSQEVIVHGKKELLGVIGSKPPHLLSSEDMEKAIKIDDMYVDVGLPKEEVEKLVSIGDIITVKREF
RELLNDNVSGKALDDRAGVVVMAVCLDELRKMYHYHDVYAVATLQEEVGVRGAITSSYNIEPDIAIAIDVTHAKARGVSR
DIEIGKGPAIGKGPNIHPAVYKGLVDIAKKYNINYQIEPLPGHSGTDAWAIQVSKKGVPTGLVSIPLKYMHTSVETANMK
DIIESGRLLAHYIANLPEELEGHLCY

Sequences:

>Translated_346_residues
MDYKELLKKLSESHGVSGHERGIYQLLKKEFEEISDEVLEDNFGNLIFKKKGLKGKYKVMLAAHLDEIGLMVKDIDEKGF
IKFTPVGGVDQRTLPSQEVIVHGKKELLGVIGSKPPHLLSSEDMEKAIKIDDMYVDVGLPKEEVEKLVSIGDIITVKREF
RELLNDNVSGKALDDRAGVVVMAVCLDELRKMYHYHDVYAVATLQEEVGVRGAITSSYNIEPDIAIAIDVTHAKARGVSR
DIEIGKGPAIGKGPNIHPAVYKGLVDIAKKYNINYQIEPLPGHSGTDAWAIQVSKKGVPTGLVSIPLKYMHTSVETANMK
DIIESGRLLAHYIANLPEELEGHLCY
>Mature_346_residues
MDYKELLKKLSESHGVSGHERGIYQLLKKEFEEISDEVLEDNFGNLIFKKKGLKGKYKVMLAAHLDEIGLMVKDIDEKGF
IKFTPVGGVDQRTLPSQEVIVHGKKELLGVIGSKPPHLLSSEDMEKAIKIDDMYVDVGLPKEEVEKLVSIGDIITVKREF
RELLNDNVSGKALDDRAGVVVMAVCLDELRKMYHYHDVYAVATLQEEVGVRGAITSSYNIEPDIAIAIDVTHAKARGVSR
DIEIGKGPAIGKGPNIHPAVYKGLVDIAKKYNINYQIEPLPGHSGTDAWAIQVSKKGVPTGLVSIPLKYMHTSVETANMK
DIIESGRLLAHYIANLPEELEGHLCY

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI87082417, Length=344, Percent_Identity=30.8139534883721, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790332, Length=322, Percent_Identity=32.2981366459627, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788727, Length=343, Percent_Identity=30.0291545189504, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 38294; Mature: 38294

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKELLKKLSESHGVSGHERGIYQLLKKEFEEISDEVLEDNFGNLIFKKKGLKGKYKVM
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEE
LAAHLDEIGLMVKDIDEKGFIKFTPVGGVDQRTLPSQEVIVHGKKELLGVIGSKPPHLLS
EEEHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHEEECHHHHHHHHCCCCCCCCC
SEDMEKAIKIDDMYVDVGLPKEEVEKLVSIGDIITVKREFRELLNDNVSGKALDDRAGVV
CHHHHHHHEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHH
VMAVCLDELRKMYHYHDVYAVATLQEEVGVRGAITSSYNIEPDIAIAIDVTHAKARGVSR
HHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCEEECCCCCCCCCEEEEEEEEHHHHCCCCC
DIEIGKGPAIGKGPNIHPAVYKGLVDIAKKYNINYQIEPLPGHSGTDAWAIQVSKKGVPT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCC
GLVSIPLKYMHTSVETANMKDIIESGRLLAHYIANLPEELEGHLCY
CHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCHHHCCCCCC
>Mature Secondary Structure
MDYKELLKKLSESHGVSGHERGIYQLLKKEFEEISDEVLEDNFGNLIFKKKGLKGKYKVM
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEE
LAAHLDEIGLMVKDIDEKGFIKFTPVGGVDQRTLPSQEVIVHGKKELLGVIGSKPPHLLS
EEEHHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHEEECHHHHHHHHCCCCCCCCC
SEDMEKAIKIDDMYVDVGLPKEEVEKLVSIGDIITVKREFRELLNDNVSGKALDDRAGVV
CHHHHHHHEECEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHH
VMAVCLDELRKMYHYHDVYAVATLQEEVGVRGAITSSYNIEPDIAIAIDVTHAKARGVSR
HHHHHHHHHHHHHHHHHHHHEEHHHHHCCCCEEECCCCCCCCCEEEEEEEEHHHHCCCCC
DIEIGKGPAIGKGPNIHPAVYKGLVDIAKKYNINYQIEPLPGHSGTDAWAIQVSKKGVPT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCC
GLVSIPLKYMHTSVETANMKDIIESGRLLAHYIANLPEELEGHLCY
CHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]