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The map label for this gene is FrvX2 [H]

Identifier: 20806951

GI number: 20806951

Start: 452862

End: 453881

Strand: Direct

Name: FrvX2 [H]

Synonym: TTE0442

Alternate gene names: 20806951

Gene position: 452862-453881 (Clockwise)

Preceding gene: 20806950

Following gene: 20806952

Centisome position: 16.84

GC content: 39.22

Gene sequence:

>1020_bases
ATGTTATTAAAGGAGTTAACTGAGCTTTTAGGTGCTTCTGGAGATGAAAAAGAGGTAAGAGAGAAGATAAAAGAGATAGT
TAAACCTTATGTGGATGAGCTTTATGTGGATAGAATTGGGAATTTGATAGCCTGCAAAAAAGGGAAAAAGGAAAAGCCAA
AGGTGATGCTGGCTGCTCACATGGATGAAGTTGCTTTAATGGTGAAGTCTGTAAATGAAGACGGGACATTAAGTTTTTCT
CCTGTGGGCGGAGTTGACAACCGTATTCTGGTAGCCAAAGCAGTAAAGGTAGGGGAGAAGAAGATAAACGGGGTAATAGG
TGCAAAACCTATACATCTTCAGAAAAAAGGAGAGCAGGAAAAACCTCTTGACTTTGATGAACTCTATATAGACATTGGAG
CAGCATCTAAAGAAGAAGCTTTAAAGCACATTTCACCCGGTGACTATGTCTACTTTGAATCAAATTTCGAGATTTTAGGG
GATGGCTATGTCAAGGCAAAGGCTTTGGACGATAGAATAGGCTGTAATGTATTAATAGAAATTTTGAAAAATACATATGA
ATATCCTGTTTGCGCGGCTTTTACAGTGCAAGAAGAGGTTGGTTTAAGAGGAGCGGGTGTTGCGGCTTATAATGTAGAGC
CTGATTTTGCGTTAGTTGTGGAAGGAACTGTAGCTGCGGATGTTGTGGATTCAGAGCCTCATCTGGTTTCCACAGAGCTG
GGAAAAGGGCCTGCAATTTCACTTATGGATAGAACTACTTTGTATGACAGAAAGATTATTGACAAGATTGTCAGGATAGC
AGAAGAGAATAATGTACCTTATCAGTTTAGGAGAATAGCGAGCGGTGGAAATGATGCAGGTAAAATACACCTTACAAAAG
GAGGAATAAAGACAGTAGCTATTTCTGTGCCTTGTAGATACATCCATTCTTTCAACTCTGTAGCTAAGCTGAGTGACTTT
GAAAATACAGTTAAGCTTGTGGACTTAGTGATTAAAAATATTGAGGAGGTATTGAAATGA

Upstream 100 bases:

>100_bases
ATACTTCTGTGGAAACTGCTAATATGAAAGACATAATAGAAAGCGGCAGACTGCTGGCACACTATATTGCCAATCTGCCT
GAGGAATTGGAGGGACATTT

Downstream 100 bases:

>100_bases
GTGTGAATGTGGAACTTATAAAAAAGCTAACACAGGCTTTTGGACCTTCGGGAAGTGAGGAGAAGGTTTTTGAGATTATA
AGAGAGGAAGTCAAAGGTTT

Product: cellulase M-like protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MLLKELTELLGASGDEKEVREKIKEIVKPYVDELYVDRIGNLIACKKGKKEKPKVMLAAHMDEVALMVKSVNEDGTLSFS
PVGGVDNRILVAKAVKVGEKKINGVIGAKPIHLQKKGEQEKPLDFDELYIDIGAASKEEALKHISPGDYVYFESNFEILG
DGYVKAKALDDRIGCNVLIEILKNTYEYPVCAAFTVQEEVGLRGAGVAAYNVEPDFALVVEGTVAADVVDSEPHLVSTEL
GKGPAISLMDRTTLYDRKIIDKIVRIAEENNVPYQFRRIASGGNDAGKIHLTKGGIKTVAISVPCRYIHSFNSVAKLSDF
ENTVKLVDLVIKNIEEVLK

Sequences:

>Translated_339_residues
MLLKELTELLGASGDEKEVREKIKEIVKPYVDELYVDRIGNLIACKKGKKEKPKVMLAAHMDEVALMVKSVNEDGTLSFS
PVGGVDNRILVAKAVKVGEKKINGVIGAKPIHLQKKGEQEKPLDFDELYIDIGAASKEEALKHISPGDYVYFESNFEILG
DGYVKAKALDDRIGCNVLIEILKNTYEYPVCAAFTVQEEVGLRGAGVAAYNVEPDFALVVEGTVAADVVDSEPHLVSTEL
GKGPAISLMDRTTLYDRKIIDKIVRIAEENNVPYQFRRIASGGNDAGKIHLTKGGIKTVAISVPCRYIHSFNSVAKLSDF
ENTVKLVDLVIKNIEEVLK
>Mature_339_residues
MLLKELTELLGASGDEKEVREKIKEIVKPYVDELYVDRIGNLIACKKGKKEKPKVMLAAHMDEVALMVKSVNEDGTLSFS
PVGGVDNRILVAKAVKVGEKKINGVIGAKPIHLQKKGEQEKPLDFDELYIDIGAASKEEALKHISPGDYVYFESNFEILG
DGYVKAKALDDRIGCNVLIEILKNTYEYPVCAAFTVQEEVGLRGAGVAAYNVEPDFALVVEGTVAADVVDSEPHLVSTEL
GKGPAISLMDRTTLYDRKIIDKIVRIAEENNVPYQFRRIASGGNDAGKIHLTKGGIKTVAISVPCRYIHSFNSVAKLSDF
ENTVKLVDLVIKNIEEVLK

Specific function: Putative aminopeptidase [H]

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=332, Percent_Identity=36.144578313253, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788727, Length=343, Percent_Identity=31.7784256559767, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI87082417, Length=333, Percent_Identity=31.5315315315315, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 37202; Mature: 37202

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKELTELLGASGDEKEVREKIKEIVKPYVDELYVDRIGNLIACKKGKKEKPKVMLAAH
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEHH
MDEVALMVKSVNEDGTLSFSPVGGVDNRILVAKAVKVGEKKINGVIGAKPIHLQKKGEQE
HHHHHHHHHHCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEECCCCCCC
KPLDFDELYIDIGAASKEEALKHISPGDYVYFESNFEILGDGYVKAKALDDRIGCNVLIE
CCCCHHHEEEEECCCCHHHHHHHCCCCCEEEEECCCEEEECCEEEEEECCCCCCHHHHHH
ILKNTYEYPVCAAFTVQEEVGLRGAGVAAYNVEPDFALVVEGTVAADVVDSEPHLVSTEL
HHHCCCCCCEEEEEEEHHHCCCCCCCEEEEECCCCEEEEEECCEEEEEECCCCCEEEECC
GKGPAISLMDRTTLYDRKIIDKIVRIAEENNVPYQFRRIASGGNDAGKIHLTKGGIKTVA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCCCEEEE
ISVPCRYIHSFNSVAKLSDFENTVKLVDLVIKNIEEVLK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLLKELTELLGASGDEKEVREKIKEIVKPYVDELYVDRIGNLIACKKGKKEKPKVMLAAH
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEHH
MDEVALMVKSVNEDGTLSFSPVGGVDNRILVAKAVKVGEKKINGVIGAKPIHLQKKGEQE
HHHHHHHHHHCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEECCCCCCC
KPLDFDELYIDIGAASKEEALKHISPGDYVYFESNFEILGDGYVKAKALDDRIGCNVLIE
CCCCHHHEEEEECCCCHHHHHHHCCCCCEEEEECCCEEEECCEEEEEECCCCCCHHHHHH
ILKNTYEYPVCAAFTVQEEVGLRGAGVAAYNVEPDFALVVEGTVAADVVDSEPHLVSTEL
HHHCCCCCCEEEEEEEHHHCCCCCCCEEEEECCCCEEEEEECCEEEEEECCCCCEEEECC
GKGPAISLMDRTTLYDRKIIDKIVRIAEENNVPYQFRRIASGGNDAGKIHLTKGGIKTVA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCCCEEEE
ISVPCRYIHSFNSVAKLSDFENTVKLVDLVIKNIEEVLK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]