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The map label for this gene is cobS

Identifier: 20806894

GI number: 20806894

Start: 405865

End: 406611

Strand: Direct

Name: cobS

Synonym: TTE0382

Alternate gene names: 20806894

Gene position: 405865-406611 (Clockwise)

Preceding gene: 20806893

Following gene: 20806895

Centisome position: 15.09

GC content: 33.6

Gene sequence:

>747_bases
ATGGAAGAACTTAAGGCTTTAATACTTTCAATACAATTTATGACAGGTATTCCCATACCAATAAATATCGATGTTAAAGA
GGATAAGATTTATAAAATAGCTTCTTATTTTCCTGTAGTAGGCCTTTTGATAGGTGGTATTTTATACATCGCTTACCTTC
TTCTAAAAGATTTGTTTTCCCGGGAAATTGTTATGACATTCCTTGTTGCTTTTTCTTACATCCTTACAAGAGGAATGCAT
ATAGATGGGCTGGCAGACACTTTTGATGGACTTTTTAGCAACAAGGACAGAGAAAAAATCATTGAAATAATGAAGGATTC
AAGGCTTGGAACAAATGGAGTTTTAGCTTTGGTTTTCATGGTGATCTTGAAAATCTTATTTTTAAGTGATATAAGACAAA
GCCTGTTGTTTTCTGCACTTTTAGTTTCACCTGTCATTGCCAGATTATCTGTGGTGTTTTCAATTGCAATAAGCAAGTCT
GCAAGAGGCGGAAAAGGGCTTGGAGGATTATTACTTGAAAGGGCTGGCTTAAGGGAATTTGTCATAGCTTTACTGATTTC
TACAATAGCAGGTTATTTTGTGATGCCTTTAAAAGACTTGGCGCTTTTATACGTGATTTCTCTTTCATTTACTTGCCTTA
TTTCAAAATATATTTCTAAAAAAATAGGTGGAATGACGGGAGACACTTTGGGAGCAGTTAATGAGTTTGTTGAGCTTATA
GCTTTTATATATTTTTCAATTTTATAA

Upstream 100 bases:

>100_bases
AGAGATATAGCAGGCAGAATGAATAAAAAAGTAGCAGAAGCATCAAAAGAGGTGTACCTTATGATATCAGGCATTCCTGT
TAAGATAAAGGGGTAATTGT

Downstream 100 bases:

>100_bases
CCTATTCGTTCGGTGTGAAAGACTCATGTGCTAAAGCAGATTTGAATTGTCACTCATATTGATGAGCTTGTTAAATTGCT
TGACAGATTAAAAGAAGAAG

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MEELKALILSIQFMTGIPIPINIDVKEDKIYKIASYFPVVGLLIGGILYIAYLLLKDLFSREIVMTFLVAFSYILTRGMH
IDGLADTFDGLFSNKDREKIIEIMKDSRLGTNGVLALVFMVILKILFLSDIRQSLLFSALLVSPVIARLSVVFSIAISKS
ARGGKGLGGLLLERAGLREFVIALLISTIAGYFVMPLKDLALLYVISLSFTCLISKYISKKIGGMTGDTLGAVNEFVELI
AFIYFSIL

Sequences:

>Translated_248_residues
MEELKALILSIQFMTGIPIPINIDVKEDKIYKIASYFPVVGLLIGGILYIAYLLLKDLFSREIVMTFLVAFSYILTRGMH
IDGLADTFDGLFSNKDREKIIEIMKDSRLGTNGVLALVFMVILKILFLSDIRQSLLFSALLVSPVIARLSVVFSIAISKS
ARGGKGLGGLLLERAGLREFVIALLISTIAGYFVMPLKDLALLYVISLSFTCLISKYISKKIGGMTGDTLGAVNEFVELI
AFIYFSIL
>Mature_248_residues
MEELKALILSIQFMTGIPIPINIDVKEDKIYKIASYFPVVGLLIGGILYIAYLLLKDLFSREIVMTFLVAFSYILTRGMH
IDGLADTFDGLFSNKDREKIIEIMKDSRLGTNGVLALVFMVILKILFLSDIRQSLLFSALLVSPVIARLSVVFSIAISKS
ARGGKGLGGLLLERAGLREFVIALLISTIAGYFVMPLKDLALLYVISLSFTCLISKYISKKIGGMTGDTLGAVNEFVELI
AFIYFSIL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=234, Percent_Identity=31.6239316239316, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_THETN (Q8RCN8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622065.1
- GeneID:   997032
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0382
- NMPDR:   fig|273068.3.peg.346
- HOGENOM:   HBG729392
- OMA:   ELTECAV
- ProtClustDB:   PRK00235
- BioCyc:   TTEN273068:TTE0382-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27351; Mature: 27351

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc4e5828)-; HASH(0xcd70aa0)-; HASH(0xca739f0)-; HASH(0xcd9bab8)-; HASH(0xb442188)-; HASH(0xc8338e4)-; HASH(0xc61dc14)-; HASH(0xb4f877c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELKALILSIQFMTGIPIPINIDVKEDKIYKIASYFPVVGLLIGGILYIAYLLLKDLFS
CHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REIVMTFLVAFSYILTRGMHIDGLADTFDGLFSNKDREKIIEIMKDSRLGTNGVLALVFM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHH
VILKILFLSDIRQSLLFSALLVSPVIARLSVVFSIAISKSARGGKGLGGLLLERAGLREF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VIALLISTIAGYFVMPLKDLALLYVISLSFTCLISKYISKKIGGMTGDTLGAVNEFVELI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFIYFSIL
HHHHHHHH
>Mature Secondary Structure
MEELKALILSIQFMTGIPIPINIDVKEDKIYKIASYFPVVGLLIGGILYIAYLLLKDLFS
CHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REIVMTFLVAFSYILTRGMHIDGLADTFDGLFSNKDREKIIEIMKDSRLGTNGVLALVFM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHH
VILKILFLSDIRQSLLFSALLVSPVIARLSVVFSIAISKSARGGKGLGGLLLERAGLREF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VIALLISTIAGYFVMPLKDLALLYVISLSFTCLISKYISKKIGGMTGDTLGAVNEFVELI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFIYFSIL
HHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11997336