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The map label for this gene is aksA

Identifier: 20094645

GI number: 20094645

Start: 1200171

End: 1201364

Strand: Reverse

Name: aksA

Synonym: MK1209

Alternate gene names: 20094645

Gene position: 1201364-1200171 (Counterclockwise)

Preceding gene: 20094646

Following gene: 20094644

Centisome position: 70.88

GC content: 65.75

Gene sequence:

>1194_bases
GTGCAGAGTCCGTACGTACGTGAAGCCGTTAGGGAGATGGACCTCCCGGACGAGGTGATCGTCTACGACACGACGCTGAG
GGACGGTGAGCAGACCCCGGGTGTCAGCTTCACGCCCGAGCAGAAGCTGGAGATCGCGCACCTACTGGACGAGCTGGGCG
TGCAGCAGATCGAGGCCGGGTTCCCGGTGGTGTCCGAGGGCGAGCGGGACGCCGTGCGTAGGATCGCGCACGAGGGACTG
AACGCCGACATTCTATGCCTGGCGAGGACGCTACGTGGTGACGTGGACGCGGCGTTGGACTGCGACGTGGACGGAGTGAT
CACCTTCATCGCCACCTCCGAGCTGCATCTGAAGCACAAGCTCCGGATGTCGAGGGAGGAGGTACTCGAGCGGATCGCCG
ACACGGTCGAGTACGCGAAGGACCACGGGCTGTGGGTGGCGTTCTCCGCGGAGGACGGGACGAGGACGGAGTTCGAGTTC
CTGGAGAGGGTGTACCGGACGGCGGAGGAGTGCGGGGCGGACCGCGTCCACGCGACGGACACCGTCGGCGTCATGATACC
GGCGGCGATGCGGCTTTTCGTGGCGAAGATCCGCGAGGTCGTGGACCTGCCGATCGGCGTGCACTGCCACGACGACTTCG
GCATGGCCGTGGCCAACTCGCTGGCCGCGGTCGAGGCGGGGGCGCAGGCGATCTCCACCACGGTGAACGGTATCGGTGAG
CGCGCGGGTAACGCAGCCCTCGAGGAGGTGATCATGGCCCTCAAGGAGCTGTACGGGATCGACCCGGGGTTCAACACCGA
GGTGCTCGCGGAGCTCTCGAGGAAGGTCTCGGAGTACTCCGGTATCGACGTGCCCCCGAACAAGGCCGTCGTGGGCGAGA
ACGCCTTCAGGCACGAGTCCGGGATCCACGTGGCCGCGGTTCTGGAGGAGCCGCGCACCTACGAGCCGATCGACCCCAAG
GAGGTGGGTATGAACCGGAAGATCGTCCTCGGTAAGCACACCGGGCGGAAGGCGGTGGTCGCGAAGTTGGAGGAACTGGG
CGTGGAACCGGAGGAGGAGATCGTCGAGGAGGTCCTCAAGCGGATCAAGGCGCTCGGGGACCGGCGTGTCAGGGTCACCG
ACTCCAAGCTCGAGGAGATCGTCCGGAATGTGCTGGAATCGAGAGGTGACCGAGACGATCCAGGTAGTCGATGA

Upstream 100 bases:

>100_bases
AAGGTCGAGCGCCTGCTGGAGCTCCTCAAAGAGCGTCTCGGCGTGGGCGTCGTAACCCTGGAGCGGTTAGAGGTCGCGTA
CGACTTGGAGGGGTGAGCAC

Downstream 100 bases:

>100_bases
GTTTCCGGGCCTCCTCCGCGTACTCCAGGAACGCGTCCGTCTTGCCGGGCCAGGACCCCTCGATCGACGCCGAAGGGTTC
GCGGCGAGCCATCTCAGGGC

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQSPYVREAVREMDLPDEVIVYDTTLRDGEQTPGVSFTPEQKLEIAHLLDELGVQQIEAGFPVVSEGERDAVRRIAHEGL
NADILCLARTLRGDVDAALDCDVDGVITFIATSELHLKHKLRMSREEVLERIADTVEYAKDHGLWVAFSAEDGTRTEFEF
LERVYRTAEECGADRVHATDTVGVMIPAAMRLFVAKIREVVDLPIGVHCHDDFGMAVANSLAAVEAGAQAISTTVNGIGE
RAGNAALEEVIMALKELYGIDPGFNTEVLAELSRKVSEYSGIDVPPNKAVVGENAFRHESGIHVAAVLEEPRTYEPIDPK
EVGMNRKIVLGKHTGRKAVVAKLEELGVEPEEEIVEEVLKRIKALGDRRVRVTDSKLEEIVRNVLESRGDRDDPGSR

Sequences:

>Translated_397_residues
MQSPYVREAVREMDLPDEVIVYDTTLRDGEQTPGVSFTPEQKLEIAHLLDELGVQQIEAGFPVVSEGERDAVRRIAHEGL
NADILCLARTLRGDVDAALDCDVDGVITFIATSELHLKHKLRMSREEVLERIADTVEYAKDHGLWVAFSAEDGTRTEFEF
LERVYRTAEECGADRVHATDTVGVMIPAAMRLFVAKIREVVDLPIGVHCHDDFGMAVANSLAAVEAGAQAISTTVNGIGE
RAGNAALEEVIMALKELYGIDPGFNTEVLAELSRKVSEYSGIDVPPNKAVVGENAFRHESGIHVAAVLEEPRTYEPIDPK
EVGMNRKIVLGKHTGRKAVVAKLEELGVEPEEEIVEEVLKRIKALGDRRVRVTDSKLEEIVRNVLESRGDRDDPGSR
>Mature_397_residues
MQSPYVREAVREMDLPDEVIVYDTTLRDGEQTPGVSFTPEQKLEIAHLLDELGVQQIEAGFPVVSEGERDAVRRIAHEGL
NADILCLARTLRGDVDAALDCDVDGVITFIATSELHLKHKLRMSREEVLERIADTVEYAKDHGLWVAFSAEDGTRTEFEF
LERVYRTAEECGADRVHATDTVGVMIPAAMRLFVAKIREVVDLPIGVHCHDDFGMAVANSLAAVEAGAQAISTTVNGIGE
RAGNAALEEVIMALKELYGIDPGFNTEVLAELSRKVSEYSGIDVPPNKAVVGENAFRHESGIHVAAVLEEPRTYEPIDPK
EVGMNRKIVLGKHTGRKAVVAKLEELGVEPEEEIVEEVLKRIKALGDRRVRVTDSKLEEIVRNVLESRGDRDDPGSR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=292, Percent_Identity=27.7397260273973, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI109150422, Length=281, Percent_Identity=25.2669039145907, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI109150427, Length=273, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1786261, Length=378, Percent_Identity=38.3597883597884, Blast_Score=246, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6320019, Length=386, Percent_Identity=31.8652849740933, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320071, Length=352, Percent_Identity=33.2386363636364, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324682, Length=363, Percent_Identity=25.8953168044077, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324225, Length=374, Percent_Identity=24.5989304812834, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582381, Length=285, Percent_Identity=25.2631578947368, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU11_METKA (Q8TW28)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614492.1
- ProteinModelPortal:   Q8TW28
- SMR:   Q8TW28
- GeneID:   1477804
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1209
- NMPDR:   fig|190192.1.peg.1205
- HOGENOM:   HBG701974
- OMA:   QIEAGFP
- ProtClustDB:   PRK11858
- BioCyc:   MKAN190192:MK1209-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_A
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02090

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 43750; Mature: 43750

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSPYVREAVREMDLPDEVIVYDTTLRDGEQTPGVSFTPEQKLEIAHLLDELGVQQIEAG
CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCC
FPVVSEGERDAVRRIAHEGLNADILCLARTLRGDVDAALDCDVDGVITFIATSELHLKHK
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCCCHHEHHHCCCHHHHHHH
LRMSREEVLERIADTVEYAKDHGLWVAFSAEDGTRTEFEFLERVYRTAEECGADRVHATD
HHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
TVGVMIPAAMRLFVAKIREVVDLPIGVHCHDDFGMAVANSLAAVEAGAQAISTTVNGIGE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGNAALEEVIMALKELYGIDPGFNTEVLAELSRKVSEYSGIDVPPNKAVVGENAFRHES
HCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHCC
GIHVAAVLEEPRTYEPIDPKEVGMNRKIVLGKHTGRKAVVAKLEELGVEPEEEIVEEVLK
CCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHH
RIKALGDRRVRVTDSKLEEIVRNVLESRGDRDDPGSR
HHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQSPYVREAVREMDLPDEVIVYDTTLRDGEQTPGVSFTPEQKLEIAHLLDELGVQQIEAG
CCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCC
FPVVSEGERDAVRRIAHEGLNADILCLARTLRGDVDAALDCDVDGVITFIATSELHLKHK
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCCCHHEHHHCCCHHHHHHH
LRMSREEVLERIADTVEYAKDHGLWVAFSAEDGTRTEFEFLERVYRTAEECGADRVHATD
HHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
TVGVMIPAAMRLFVAKIREVVDLPIGVHCHDDFGMAVANSLAAVEAGAQAISTTVNGIGE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAGNAALEEVIMALKELYGIDPGFNTEVLAELSRKVSEYSGIDVPPNKAVVGENAFRHES
HCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHCC
GIHVAAVLEEPRTYEPIDPKEVGMNRKIVLGKHTGRKAVVAKLEELGVEPEEEIVEEVLK
CCEEEEEECCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHH
RIKALGDRRVRVTDSKLEEIVRNVLESRGDRDDPGSR
HHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014