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The map label for this gene is HyaB [C]

Identifier: 20094638

GI number: 20094638

Start: 1195047

End: 1195664

Strand: Reverse

Name: HyaB [C]

Synonym: MK1202

Alternate gene names: 20094638

Gene position: 1195664-1195047 (Counterclockwise)

Preceding gene: 20094639

Following gene: 20094637

Centisome position: 70.54

GC content: 62.46

Gene sequence:

>618_bases
CTGGATTCGTGGAGTGAGAAACTGGACGTAGACGTGTGGGAACTCTTGAAACCGTGTGCCCGTAACAGGAACATAGCGAG
GATACTGGAGGCGTTGCTCCTGACGCGTCTGGCCTTACACTGGATCGACGACCTCCCCACCACGGGCACCACGTACAAGG
ATCCCTCGCGCCGCTCCGGGTGCTCCGGTACCGGCGTCATCGAGGCCCCGAGGGGTACTCTGGTCCACAGGGTGTCCGTC
GGATCGGATGGTAAGGTGTTCAACTACGAGATAATCACGTCGACGAACCTGAACCACGCGCCGATAGAGGAGTCCATGGT
GGGCGAGAGGGTCTACGTGGACGAGAAGTTGGCGGCCGCGAAGCGTCAGTTATCCGAGTCGGAGCGCTTCCTGCTCGGCG
ACGCATGCAGGGCGGCTCGGAGCTTCAACCCGTGCAACTCCTGCGCGCCCCACGCGATCGTGAAGGTGGTGAAGAAGGGG
TGGACGAACTCCGGAGCTCCCACTGAGGTCCCACGTGGAGGAACTGAGGAGGAGAGTGCTGAGGTCATTAGTGGCCGTGG
CGCTCACGTCGAGCGTGGAGTTCCCCTCCGACGAGATGCTGCGCCGGGCCGTCCATGA

Upstream 100 bases:

>100_bases
TATTACGAGTGGAACGGTACCAAGTACGCGTGTGAGGCGGGACCGTTGACCAGGTTTAGCGGTAGCTTACAAGGCCGGGG
ATGAAACCGTCAGGTGGTTC

Downstream 100 bases:

>100_bases
TCCTCTACGAGGTAGGGGTACTAACCGCGAAAAAGGAGTGAAAAGAGGATGATAGACGGGCTGGGCACCTCGGAACTGCT
GCTGATCCTGGCGGTGATAT

Product: Ni,Fe-hydrogenase I large subunit

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MDSWSEKLDVDVWELLKPCARNRNIARILEALLLTRLALHWIDDLPTTGTTYKDPSRRSGCSGTGVIEAPRGTLVHRVSV
GSDGKVFNYEIITSTNLNHAPIEESMVGERVYVDEKLAAAKRQLSESERFLLGDACRAARSFNPCNSCAPHAIVKVVKKG
WTNSGAPTEVPRGGTEEESAEVISGRGAHVERGVPLRRDAAPGRP

Sequences:

>Translated_205_residues
MDSWSEKLDVDVWELLKPCARNRNIARILEALLLTRLALHWIDDLPTTGTTYKDPSRRSGCSGTGVIEAPRGTLVHRVSV
GSDGKVFNYEIITSTNLNHAPIEESMVGERVYVDEKLAAAKRQLSESERFLLGDACRAARSFNPCNSCAPHAIVKVVKKG
WTNSGAPTEVPRGGTEEESAEVISGRGAHVERGVPLRRDAAPGRP
>Mature_205_residues
MDSWSEKLDVDVWELLKPCARNRNIARILEALLLTRLALHWIDDLPTTGTTYKDPSRRSGCSGTGVIEAPRGTLVHRVSV
GSDGKVFNYEIITSTNLNHAPIEESMVGERVYVDEKLAAAKRQLSESERFLLGDACRAARSFNPCNSCAPHAIVKVVKKG
WTNSGAPTEVPRGGTEEESAEVISGRGAHVERGVPLRRDAAPGRP

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 22386; Mature: 22386

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSWSEKLDVDVWELLKPCARNRNIARILEALLLTRLALHWIDDLPTTGTTYKDPSRRSG
CCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
CSGTGVIEAPRGTLVHRVSVGSDGKVFNYEIITSTNLNHAPIEESMVGERVYVDEKLAAA
CCCCCEEECCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHH
KRQLSESERFLLGDACRAARSFNPCNSCAPHAIVKVVKKGWTNSGAPTEVPRGGTEEESA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
EVISGRGAHVERGVPLRRDAAPGRP
HHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDSWSEKLDVDVWELLKPCARNRNIARILEALLLTRLALHWIDDLPTTGTTYKDPSRRSG
CCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
CSGTGVIEAPRGTLVHRVSVGSDGKVFNYEIITSTNLNHAPIEESMVGERVYVDEKLAAA
CCCCCEEECCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHH
KRQLSESERFLLGDACRAARSFNPCNSCAPHAIVKVVKKGWTNSGAPTEVPRGGTEEESA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
EVISGRGAHVERGVPLRRDAAPGRP
HHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA