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The map label for this gene is PurB [H]

Identifier: 20094521

GI number: 20094521

Start: 1054126

End: 1055544

Strand: Reverse

Name: PurB [H]

Synonym: MK1085

Alternate gene names: 20094521

Gene position: 1055544-1054126 (Counterclockwise)

Preceding gene: 20094522

Following gene: 20094520

Centisome position: 62.28

GC content: 62.86

Gene sequence:

>1419_bases
TTGCCCGTTCATCCGATCGAGTCACGGTACGGATCGGACGAGCTCCGCCGGGTGTTCTCGGAAGAGAACAAAGTGGCGAA
GATGCTCGAGGTTGAGGCGGCCCTGGTACGGGCGCTCTCCGAGGTGTTCGACTTCGTACCGGAGGAGGCCGCGGACGAGG
TGGAACGCGTCGTCGAGGAGATCACCGGCGACGAGGAAGAGCTACGACGGTTCGTGGAACGCGTCAAGGAGATCGAGGCG
GAGATCAAACACGACGTGATGGCCCTGGTGAAGGCGCTATCGGAGCGGTGCGAGGTTGGCGGGGACTACGTGCACCTGGG
CGCTACCAGCAACGACGTGATAGACACCGCGCACGCGTTGGTACTGCGGGAAGCACTGTCGATAATCTACCGCAGGCTGC
ACCGTCTAGCGGAAGTGCTGGCGGAGAAGGCCGAGGAGTACGCCGACCTCCCGATGGTGGGTCGCACGCACGGTCAGCAC
GCGGTTCCCACGACGCTCGGTATGAAGTTCGCGATCTGGGCACGCGAGGTCGTGAGGCACCTGAAGCGACTCCGGGAGTG
CGCGAACCGGGTACTGGTGGGGCAGCTGTCCGGTGCCGTCGGAACGATGGCGGCGCTGGGCGAGAAGGGTCCTGAGGTGC
AGCGTCGGGTGATGGAACTGCTGAACCTCAGGCCGGTGACCGTGTCCAACCAGGTCATTCAGCGGGACCGCTACGCGGAG
TTGATCGCACTGCTCGCGCTGATCGGCTCGACGCTGGATAAGATCGGCCGCGAGATCAGGAACCTCCAGCGGACGGAGAT
CCGGGAGGTCGAGGAACCGTTCGACCCCGAGAAGCAGGTCGGATCCTCGACGATGCCACACAAGCGGAATCCGATCCGTT
CCGAGCGGGTGTGTAGCCTGGCGAGGGTACTGCGGTCAAACGTGCAGATAGCGCTCGAGAACGTGCCGCTCGAACACGAG
CGGGACCTCACGAACTCCGCGAGCGAGCGGGTGATCCTCCCCGAACAGTTCCTGCTGCTGGACGAAATGCTGCGGCTGAC
GATCCACAACTTGGAGGGATTACGCGTTTACGAGGAGAACATACGGGAGAACCTGCGGCTGACGAAGGGGCTTAATATGG
CGGAGGCACTGATGGTCGAACTGGTCAAGCGAGGAATAGGGCGACAGGAGGCGCACGAGCTCGTGAGACGGCTCGCGATG
AGGGCTTGGGAGGAAGGACGTGACTTCGCGGAAGTAGTTAAGGAGGAAGAGCGCGTCCGTGAGCTGTTCGGAGAAGAAGA
GTTAGATGAGGTGTTGGACCCCGAGAAGTACTTGGGCGTGGCTCCCGACCTGGCGCGTGAGGCCGCCGAGAAGACCCGAC
GCGACCTGGAGGAGATCGATCGCGAGATGAAGGAGGTGTTGGGTGTCGGTGCGGGATAA

Upstream 100 bases:

>100_bases
TGCGCCGGTGAAGGGATAACTTACCCCCGATGACGAAAGGGTAGGTTGCCGTCGACCACGAGGGATGCGGAGAGCCGTCC
CGACCGGAGGGGGCTCTGAG

Downstream 100 bases:

>100_bases
AGTCCGGGTCCTTCGGTGTGATTGCGCGCGCGTCCTCGAGGTGGAGGGCGTAGAAACCCCGGCTCCCTTCCTCATCCCCG
AACGACTGTCCGTCGCCGTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPVHPIESRYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFDFVPEEAADEVERVVEEITGDEEELRRFVERVKEIEA
EIKHDVMALVKALSERCEVGGDYVHLGATSNDVIDTAHALVLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQH
AVPTTLGMKFAIWAREVVRHLKRLRECANRVLVGQLSGAVGTMAALGEKGPEVQRRVMELLNLRPVTVSNQVIQRDRYAE
LIALLALIGSTLDKIGREIRNLQRTEIREVEEPFDPEKQVGSSTMPHKRNPIRSERVCSLARVLRSNVQIALENVPLEHE
RDLTNSASERVILPEQFLLLDEMLRLTIHNLEGLRVYEENIRENLRLTKGLNMAEALMVELVKRGIGRQEAHELVRRLAM
RAWEEGRDFAEVVKEEERVRELFGEEELDEVLDPEKYLGVAPDLAREAAEKTRRDLEEIDREMKEVLGVGAG

Sequences:

>Translated_472_residues
MPVHPIESRYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFDFVPEEAADEVERVVEEITGDEEELRRFVERVKEIEA
EIKHDVMALVKALSERCEVGGDYVHLGATSNDVIDTAHALVLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQH
AVPTTLGMKFAIWAREVVRHLKRLRECANRVLVGQLSGAVGTMAALGEKGPEVQRRVMELLNLRPVTVSNQVIQRDRYAE
LIALLALIGSTLDKIGREIRNLQRTEIREVEEPFDPEKQVGSSTMPHKRNPIRSERVCSLARVLRSNVQIALENVPLEHE
RDLTNSASERVILPEQFLLLDEMLRLTIHNLEGLRVYEENIRENLRLTKGLNMAEALMVELVKRGIGRQEAHELVRRLAM
RAWEEGRDFAEVVKEEERVRELFGEEELDEVLDPEKYLGVAPDLAREAAEKTRRDLEEIDREMKEVLGVGAG
>Mature_471_residues
PVHPIESRYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFDFVPEEAADEVERVVEEITGDEEELRRFVERVKEIEAE
IKHDVMALVKALSERCEVGGDYVHLGATSNDVIDTAHALVLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQHA
VPTTLGMKFAIWAREVVRHLKRLRECANRVLVGQLSGAVGTMAALGEKGPEVQRRVMELLNLRPVTVSNQVIQRDRYAEL
IALLALIGSTLDKIGREIRNLQRTEIREVEEPFDPEKQVGSSTMPHKRNPIRSERVCSLARVLRSNVQIALENVPLEHER
DLTNSASERVILPEQFLLLDEMLRLTIHNLEGLRVYEENIRENLRLTKGLNMAEALMVELVKRGIGRQEAHELVRRLAMR
AWEEGRDFAEVVKEEERVRELFGEEELDEVLDPEKYLGVAPDLAREAAEKTRRDLEEIDREMKEVLGVGAG

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=475, Percent_Identity=32.4210526315789, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI183227688, Length=395, Percent_Identity=32.9113924050633, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI19743875, Length=347, Percent_Identity=26.8011527377522, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1787376, Length=354, Percent_Identity=27.9661016949153, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787896, Length=351, Percent_Identity=27.9202279202279, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790398, Length=353, Percent_Identity=24.6458923512748, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=334, Percent_Identity=32.6347305389222, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32564234, Length=275, Percent_Identity=32.3636363636364, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17553882, Length=349, Percent_Identity=29.7994269340974, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI32565146, Length=342, Percent_Identity=29.8245614035088, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=463, Percent_Identity=34.341252699784, Blast_Score=219, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6324993, Length=350, Percent_Identity=25.7142857142857, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647570, Length=460, Percent_Identity=32.1739130434783, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI78710009, Length=349, Percent_Identity=29.2263610315186, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24640179, Length=352, Percent_Identity=29.8295454545455, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640177, Length=352, Percent_Identity=29.8295454545455, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24662684, Length=326, Percent_Identity=27.9141104294479, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583245, Length=349, Percent_Identity=27.2206303724928, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221473854, Length=438, Percent_Identity=24.2009132420091, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI78706858, Length=438, Percent_Identity=24.2009132420091, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53803; Mature: 53672

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVHPIESRYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFDFVPEEAADEVERVVEE
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ITGDEEELRRFVERVKEIEAEIKHDVMALVKALSERCEVGGDYVHLGATSNDVIDTAHAL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
VLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQHAVPTTLGMKFAIWAREVVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LKRLRECANRVLVGQLSGAVGTMAALGEKGPEVQRRVMELLNLRPVTVSNQVIQRDRYAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LIALLALIGSTLDKIGREIRNLQRTEIREVEEPFDPEKQVGSSTMPHKRNPIRSERVCSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHH
ARVLRSNVQIALENVPLEHERDLTNSASERVILPEQFLLLDEMLRLTIHNLEGLRVYEEN
HHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCHHHHHHHH
IRENLRLTKGLNMAEALMVELVKRGIGRQEAHELVRRLAMRAWEEGRDFAEVVKEEERVR
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELFGEEELDEVLDPEKYLGVAPDLAREAAEKTRRDLEEIDREMKEVLGVGAG
HHHCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PVHPIESRYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFDFVPEEAADEVERVVEE
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ITGDEEELRRFVERVKEIEAEIKHDVMALVKALSERCEVGGDYVHLGATSNDVIDTAHAL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
VLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQHAVPTTLGMKFAIWAREVVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LKRLRECANRVLVGQLSGAVGTMAALGEKGPEVQRRVMELLNLRPVTVSNQVIQRDRYAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LIALLALIGSTLDKIGREIRNLQRTEIREVEEPFDPEKQVGSSTMPHKRNPIRSERVCSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHH
ARVLRSNVQIALENVPLEHERDLTNSASERVILPEQFLLLDEMLRLTIHNLEGLRVYEEN
HHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCHHHHHHHH
IRENLRLTKGLNMAEALMVELVKRGIGRQEAHELVRRLAMRAWEEGRDFAEVVKEEERVR
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELFGEEELDEVLDPEKYLGVAPDLAREAAEKTRRDLEEIDREMKEVLGVGAG
HHHCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]