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The map label for this gene is SIR2

Identifier: 20094511

GI number: 20094511

Start: 1042423

End: 1043175

Strand: Direct

Name: SIR2

Synonym: MK1075

Alternate gene names: 20094511

Gene position: 1042423-1043175 (Clockwise)

Preceding gene: 20094510

Following gene: 20094513

Centisome position: 61.5

GC content: 66.67

Gene sequence:

>753_bases
TTGCCACTCCGCGAGGCGGCCGAGAGGGTTGTAGAGGCCGAGACAGTCGTCGTGCTCACCGGGGCCGGCGCCTCGGCGGA
CTCCGGTATCCCGACGTTCCGGGGAAAGGATGGACTCTGGAACAAGTACGACCCTCGGGAACTGGCCACACCCGAGGCCT
TCGCACGCGATCCGGAGAAGGTTTGGGAGTGGTACCTCTGGCGCCGCCGTAAGATCGCCGAGGCCGAACCCAACCCTGCC
CACACCGTCCTGGCGCGTATGGAGCGGGACGGCCTCCTCGAAGCCGTCATCACGCAGAACGTGGACGGGCTCCACCAGCG
GGCCGGATCCCGTCGCGTGATCGAGCTCCACGGCAACATCTGGCGGGACGAGTGCGTATCCTGCGAATACCAGCGAGTGA
ACGACCCCGAGCGCGGCGAGGGACTCGAGTACGACGAGCTGCCACCGAGGTGCCCCGAGTGCGGGGATCCGCTGCGCCCC
GGCGTAGTCTGGTTCGGTGAGCCACTGCCGTCTGACGCGCTCGTGGAGGCGGAGAACCTCGCCCGATCCTGCGACGTGAT
GCTCGTGATCGGAACCTCGGGTGAAGTCCGGCCCGCCGCCGACCTCCCGCTCGTGGCCAAATCCTGTGGGGCCACCCTGA
TAGAGATCAACCCGAGCGAGACTGCGCTCAGCCCCCACATGGACGTTATCATCCGAGAACGAGCGGCTTCGGCCATGGAA
GCCCTATGGAACGAGATCGAGCGACTCCTGTGA

Upstream 100 bases:

>100_bases
TACCGTTCTGCCCCTATAACTCCATACACCGTGAAGAGATCGAGCGGAAGTTCGGCGTGCCGCTCGAGGAGTGGAAGGAA
CGCCGGAACGGGTGACACGG

Downstream 100 bases:

>100_bases
TGATATGTGGATTTTCCTCCGGATATGGGCCCCTACCGGCCGGTTTCCATGTTCCTGCGCGATGATTTGTGCGATACCAC
CTCGCAACTTCTACTCTCAA

Product: Sir2 family NAD-dependent protein deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPLREAAERVVEAETVVVLTGAGASADSGIPTFRGKDGLWNKYDPRELATPEAFARDPEKVWEWYLWRRRKIAEAEPNPA
HTVLARMERDGLLEAVITQNVDGLHQRAGSRRVIELHGNIWRDECVSCEYQRVNDPERGEGLEYDELPPRCPECGDPLRP
GVVWFGEPLPSDALVEAENLARSCDVMLVIGTSGEVRPAADLPLVAKSCGATLIEINPSETALSPHMDVIIRERAASAME
ALWNEIERLL

Sequences:

>Translated_250_residues
MPLREAAERVVEAETVVVLTGAGASADSGIPTFRGKDGLWNKYDPRELATPEAFARDPEKVWEWYLWRRRKIAEAEPNPA
HTVLARMERDGLLEAVITQNVDGLHQRAGSRRVIELHGNIWRDECVSCEYQRVNDPERGEGLEYDELPPRCPECGDPLRP
GVVWFGEPLPSDALVEAENLARSCDVMLVIGTSGEVRPAADLPLVAKSCGATLIEINPSETALSPHMDVIIRERAASAME
ALWNEIERLL
>Mature_249_residues
PLREAAERVVEAETVVVLTGAGASADSGIPTFRGKDGLWNKYDPRELATPEAFARDPEKVWEWYLWRRRKIAEAEPNPAH
TVLARMERDGLLEAVITQNVDGLHQRAGSRRVIELHGNIWRDECVSCEYQRVNDPERGEGLEYDELPPRCPECGDPLRPG
VVWFGEPLPSDALVEAENLARSCDVMLVIGTSGEVRPAADLPLVAKSCGATLIEINPSETALSPHMDVIIRERAASAMEA
LWNEIERLL

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI13787215, Length=231, Percent_Identity=39.8268398268398, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI6912664, Length=231, Percent_Identity=39.8268398268398, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI300795542, Length=220, Percent_Identity=40, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI7657575, Length=214, Percent_Identity=32.2429906542056, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI300797577, Length=251, Percent_Identity=32.2709163346614, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI6912660, Length=222, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI6912662, Length=249, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI300797597, Length=244, Percent_Identity=31.1475409836066, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI63054862, Length=196, Percent_Identity=34.6938775510204, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI7706712, Length=207, Percent_Identity=33.8164251207729, Blast_Score=100, Evalue=9e-22,
Organism=Homo sapiens, GI13775602, Length=210, Percent_Identity=30, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI13775600, Length=210, Percent_Identity=30, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI300797705, Length=208, Percent_Identity=29.3269230769231, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI215982798, Length=157, Percent_Identity=32.484076433121, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI308199517, Length=224, Percent_Identity=42.4107142857143, Blast_Score=147, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17567771, Length=225, Percent_Identity=32, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71990482, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17541892, Length=211, Percent_Identity=29.8578199052133, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=224, Percent_Identity=29.4642857142857, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17505510, Length=258, Percent_Identity=29.4573643410853, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324504, Length=229, Percent_Identity=26.6375545851528, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320163, Length=257, Percent_Identity=25.2918287937743, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325242, Length=215, Percent_Identity=30.2325581395349, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17137536, Length=226, Percent_Identity=30.5309734513274, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28571445, Length=260, Percent_Identity=31.1538461538462, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24648389, Length=203, Percent_Identity=32.0197044334975, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645650, Length=258, Percent_Identity=28.2945736434109, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571443, Length=200, Percent_Identity=31.5, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571441, Length=200, Percent_Identity=31.5, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650933, Length=204, Percent_Identity=28.4313725490196, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_METKA (Q8TWG0)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614358.1
- ProteinModelPortal:   Q8TWG0
- SMR:   Q8TWG0
- GeneID:   1477176
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1075
- NMPDR:   fig|190192.1.peg.1071
- HOGENOM:   HBG641281
- OMA:   DADGLWE
- BioCyc:   MKAN190192:MK1075-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27800; Mature: 27669

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLREAAERVVEAETVVVLTGAGASADSGIPTFRGKDGLWNKYDPRELATPEAFARDPEK
CCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCHHH
VWEWYLWRRRKIAEAEPNPAHTVLARMERDGLLEAVITQNVDGLHQRAGSRRVIELHGNI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
WRDECVSCEYQRVNDPERGEGLEYDELPPRCPECGDPLRPGVVWFGEPLPSDALVEAENL
CHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHH
ARSCDVMLVIGTSGEVRPAADLPLVAKSCGATLIEINPSETALSPHMDVIIRERAASAME
HHHCCEEEEECCCCCCCCCCCCCHHHHHCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHH
ALWNEIERLL
HHHHHHHHHC
>Mature Secondary Structure 
PLREAAERVVEAETVVVLTGAGASADSGIPTFRGKDGLWNKYDPRELATPEAFARDPEK
CHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCHHH
VWEWYLWRRRKIAEAEPNPAHTVLARMERDGLLEAVITQNVDGLHQRAGSRRVIELHGNI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
WRDECVSCEYQRVNDPERGEGLEYDELPPRCPECGDPLRPGVVWFGEPLPSDALVEAENL
CHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHH
ARSCDVMLVIGTSGEVRPAADLPLVAKSCGATLIEINPSETALSPHMDVIIRERAASAME
HHHCCEEEEECCCCCCCCCCCCCHHHHHCCCEEEEECCCHHCCCCCHHHHHHHHHHHHHH
ALWNEIERLL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014