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The map label for this gene is PDX2

Identifier: 20094498

GI number: 20094498

Start: 1030519

End: 1031127

Strand: Direct

Name: PDX2

Synonym: MK1062

Alternate gene names: 20094498

Gene position: 1030519-1031127 (Clockwise)

Preceding gene: 20094497

Following gene: 20094499

Centisome position: 60.8

GC content: 62.4

Gene sequence:

>609_bases
TTGAAGGTCGCTGTCGTCGCCGTGCAGGGAGCCGTCGAGGAACACGAATCGATCCTGGAAGCGGCCGGTGAGCGGATCGG
CGAAGACGTCGAGGTGGTATGGGCAAGGTACCCGGAAGATCTCGAGGACGTGGACGCCGTCGTGATTCCGGGAGGAGAGA
GCACCACGATCGGACGTCTGATGGAGCGGCACGACCTGGTTAAGCCGCTGCTGGAGCTGGCGGAGTCGGATACTCCCATC
CTTGGAACCTGCGCGGGGATGGTCATCCTCGCGCGTGAGGTCGTTCCGCAGGCTCATCCAGGGACGGAGGTGGAGATCGA
GCAGCCTCTACTAGGTCTAATGGACGTGCGGGTAGTCCGGAACGCGTTCGGCCGGCAGCGTGAATCATTCGAAGTAGATA
TCGAGATCGAGGGGCTCGAGGACCGGTTCCGGGCAGTCTTCATCCGAGCTCCGGCCGTGGACGAGGTCCTGTCCGACGAT
GTGAAGGTGCTCGCGGAGTACGGCGATTACATTGTGGCCGTGGAGCAGGATCACCTGCTCGCCACGGCTTTCCACCCGGA
GCTCACCGACGATCCGCGTCTTCACGCTTACTTCCTGGAGAAGGTGTGA

Upstream 100 bases:

>100_bases
CCGAGGAGGGCTTGCATACTGTTTGAAGCGTTAACGGCTGGTCGCCACGGCCGGTGCGTATTATATCCTTCCGACTGTCG
GGTGCTGGGGGCCGTCCGGC

Downstream 100 bases:

>100_bases
GGGGAACTGACCTTGGCGTGCGGCATCGCCGGTATCGTGCTGCCCGAGCCGGGGCCTGTCGGTGCTATCCTGACGGAGAT
GCTGGATGCGCTGCAGCACC

Product: pyridoxine biosynthesis glutamine amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKVAVVAVQGAVEEHESILEAAGERIGEDVEVVWARYPEDLEDVDAVVIPGGESTTIGRLMERHDLVKPLLELAESDTPI
LGTCAGMVILAREVVPQAHPGTEVEIEQPLLGLMDVRVVRNAFGRQRESFEVDIEIEGLEDRFRAVFIRAPAVDEVLSDD
VKVLAEYGDYIVAVEQDHLLATAFHPELTDDPRLHAYFLEKV

Sequences:

>Translated_202_residues
MKVAVVAVQGAVEEHESILEAAGERIGEDVEVVWARYPEDLEDVDAVVIPGGESTTIGRLMERHDLVKPLLELAESDTPI
LGTCAGMVILAREVVPQAHPGTEVEIEQPLLGLMDVRVVRNAFGRQRESFEVDIEIEGLEDRFRAVFIRAPAVDEVLSDD
VKVLAEYGDYIVAVEQDHLLATAFHPELTDDPRLHAYFLEKV
>Mature_202_residues
MKVAVVAVQGAVEEHESILEAAGERIGEDVEVVWARYPEDLEDVDAVVIPGGESTTIGRLMERHDLVKPLLELAESDTPI
LGTCAGMVILAREVVPQAHPGTEVEIEQPLLGLMDVRVVRNAFGRQRESFEVDIEIEGLEDRFRAVFIRAPAVDEVLSDD
VKVLAEYGDYIVAVEQDHLLATAFHPELTDDPRLHAYFLEKV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=219, Percent_Identity=37.4429223744292, Blast_Score=121, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6323995, Length=215, Percent_Identity=32.5581395348837, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321048, Length=215, Percent_Identity=32.093023255814, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_METKA (Q8TWH2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614345.1
- ProteinModelPortal:   Q8TWH2
- SMR:   Q8TWH2
- GeneID:   1477163
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1062
- NMPDR:   fig|190192.1.peg.1058
- HOGENOM:   HBG292341
- OMA:   DKWSING
- BioCyc:   MKAN190192:MK1062-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 22378; Mature: 22378

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 84-84 ACT_SITE 185-185 ACT_SITE 187-187 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVVAVQGAVEEHESILEAAGERIGEDVEVVWARYPEDLEDVDAVVIPGGESTTIGRL
CEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEECCCCCHHHHHH
MERHDLVKPLLELAESDTPILGTCAGMVILAREVVPQAHPGTEVEIEQPLLGLMDVRVVR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHH
NAFGRQRESFEVDIEIEGLEDRFRAVFIRAPAVDEVLSDDVKVLAEYGDYIVAVEQDHLL
HHHCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCEE
ATAFHPELTDDPRLHAYFLEKV
EEEECCCCCCCCCHHHHEECCC
>Mature Secondary Structure
MKVAVVAVQGAVEEHESILEAAGERIGEDVEVVWARYPEDLEDVDAVVIPGGESTTIGRL
CEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEECCCCCHHHHHH
MERHDLVKPLLELAESDTPILGTCAGMVILAREVVPQAHPGTEVEIEQPLLGLMDVRVVR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHH
NAFGRQRESFEVDIEIEGLEDRFRAVFIRAPAVDEVLSDDVKVLAEYGDYIVAVEQDHLL
HHHCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCEE
ATAFHPELTDDPRLHAYFLEKV
EEEECCCCCCCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014