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The map label for this gene is GltB_2 [C]

Identifier: 20094499

GI number: 20094499

Start: 1031140

End: 1032081

Strand: Direct

Name: GltB_2 [C]

Synonym: MK1063

Alternate gene names: 20094499

Gene position: 1031140-1032081 (Clockwise)

Preceding gene: 20094498

Following gene: 20094500

Centisome position: 60.84

GC content: 61.36

Gene sequence:

>942_bases
TTGGCGTGCGGCATCGCCGGTATCGTGCTGCCCGAGCCGGGGCCTGTCGGTGCTATCCTGACGGAGATGCTGGATGCGCT
GCAGCACCGGGGTCCCGACTCGGCCGGTTACGGTCTGTACCGGGACGTCGACACGGCGGTTTTCGTCCTAGAGCTGCCGG
CCGAGGACGGGGAAATGGAGCTGCTGAGTGAGGTCGAGCGTGCGCTGGAAGGGCGCCAACTGAAACGGGTGGAGCGGATC
GCCGAAGACGCGGGCACGCGGGTGTACCGGCTGTTCGTGGAGGGGTTCTCGACGGGACGACAGGGCCAGCGAGAGCTGGC
GGAAGTCGTGGAGAAGATCGAGAACTCGGACATCACCGTATTGAGCGCGGGTCACGGCTTCGAGATCCTGAAGGACGTGG
GCACGGCGGCCGAAGTCTCGGAGCAGTACGGTGTCGAGGCGATCGAAGGCACTCACGGGATCGGACACGTGAGGTTCTCG
ACGGAATCGGAGGTCGATAGGTATCACGCGCATCCGTTTCAAAGTTACATGATACCTAACATGGCAGTGGTTCACAATGG
TCAAATTACCAACTACTACACGATCAGAGAGCGACTCGAAATTAAAGGATATCAGTTCAAGACCAACAACGATTCGGAGT
GTATAGTAGTGTATGTAGCGGACAAGTTGCGGGACGGATACTCGCTGGAGGAGGCGATGGAGGAGGCCATCCGCGACCTC
GACGGACCGTTCTGCTTTATTATCAGCACTCCGGACGCGATCGCGGTCGCGAGGGACCCGCTGGGGCTTCGTCCCGGTGT
GATCGGGTTCGGTAAGGACGGGGCTGTCGCCGTCGCCTCGGAGGAGGTCGCGCTGCGTCGGATCTTCGGCGACGAGCTCG
AAGGTATCGAACAGATCGAACCGGGCGAGTACGAGGTGTTCGAGGTGGGTGGCCGGGGTTGA

Upstream 100 bases:

>100_bases
GGAGCAGGATCACCTGCTCGCCACGGCTTTCCACCCGGAGCTCACCGACGATCCGCGTCTTCACGCTTACTTCCTGGAGA
AGGTGTGAGGGGAACTGACC

Downstream 100 bases:

>100_bases
AGGAGAGGGTTATCGACTGTCGTGACAAGGAACCTCGGGACATCAACAGCGCGCTGAAGACCTACGCCCGGGAGTACGAC
CGGATCGTGCTCGAGAACCC

Product: glutamate synthase subunit 1

Products: L-glutamine; 5-phospho-alpha-D-ribose 1-diphosphate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MACGIAGIVLPEPGPVGAILTEMLDALQHRGPDSAGYGLYRDVDTAVFVLELPAEDGEMELLSEVERALEGRQLKRVERI
AEDAGTRVYRLFVEGFSTGRQGQRELAEVVEKIENSDITVLSAGHGFEILKDVGTAAEVSEQYGVEAIEGTHGIGHVRFS
TESEVDRYHAHPFQSYMIPNMAVVHNGQITNYYTIRERLEIKGYQFKTNNDSECIVVYVADKLRDGYSLEEAMEEAIRDL
DGPFCFIISTPDAIAVARDPLGLRPGVIGFGKDGAVAVASEEVALRRIFGDELEGIEQIEPGEYEVFEVGGRG

Sequences:

>Translated_313_residues
MACGIAGIVLPEPGPVGAILTEMLDALQHRGPDSAGYGLYRDVDTAVFVLELPAEDGEMELLSEVERALEGRQLKRVERI
AEDAGTRVYRLFVEGFSTGRQGQRELAEVVEKIENSDITVLSAGHGFEILKDVGTAAEVSEQYGVEAIEGTHGIGHVRFS
TESEVDRYHAHPFQSYMIPNMAVVHNGQITNYYTIRERLEIKGYQFKTNNDSECIVVYVADKLRDGYSLEEAMEEAIRDL
DGPFCFIISTPDAIAVARDPLGLRPGVIGFGKDGAVAVASEEVALRRIFGDELEGIEQIEPGEYEVFEVGGRG
>Mature_312_residues
ACGIAGIVLPEPGPVGAILTEMLDALQHRGPDSAGYGLYRDVDTAVFVLELPAEDGEMELLSEVERALEGRQLKRVERIA
EDAGTRVYRLFVEGFSTGRQGQRELAEVVEKIENSDITVLSAGHGFEILKDVGTAAEVSEQYGVEAIEGTHGIGHVRFST
ESEVDRYHAHPFQSYMIPNMAVVHNGQITNYYTIRERLEIKGYQFKTNNDSECIVVYVADKLRDGYSLEEAMEEAIRDLD
GPFCFIISTPDAIAVARDPLGLRPGVIGFGKDGAVAVASEEVALRRIFGDELEGIEQIEPGEYEVFEVGGRG

Specific function: Catalyzes The First Step In Hexosamine Metabolism, Converting Fructose-6p Into Glucosamine-6p Using Glutamine As A Nitrogen Source. [C]

COG id: COG0067

COG function: function code E; Glutamate synthase domain 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=30.1310043668122, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788651, Length=200, Percent_Identity=30.5, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25147560, Length=132, Percent_Identity=34.0909090909091, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25147557, Length=132, Percent_Identity=34.0909090909091, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993933, Length=132, Percent_Identity=34.0909090909091, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532897, Length=132, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=132, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357745, Length=97, Percent_Identity=38.1443298969072, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: 2.4.2.14

Molecular weight: Translated: 34315; Mature: 34184

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACGIAGIVLPEPGPVGAILTEMLDALQHRGPDSAGYGLYRDVDTAVFVLELPAEDGEME
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEECCCCCHHH
LLSEVERALEGRQLKRVERIAEDAGTRVYRLFVEGFSTGRQGQRELAEVVEKIENSDITV
HHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHCCCCEEE
LSAGHGFEILKDVGTAAEVSEQYGVEAIEGTHGIGHVRFSTESEVDRYHAHPFQSYMIPN
EECCCCHHHHHHCCCHHHHHHHHCCEEECCCCCCEEEEECCHHHHHHHHCCCHHHHCCCC
MAVVHNGQITNYYTIRERLEIKGYQFKTNNDSECIVVYVADKLRDGYSLEEAMEEAIRDL
EEEEECCCCCEEEEEEEHHEECCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHC
DGPFCFIISTPDAIAVARDPLGLRPGVIGFGKDGAVAVASEEVALRRIFGDELEGIEQIE
CCCEEEEEECCCEEEEECCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHCCHHHCC
PGEYEVFEVGGRG
CCCEEEEEECCCC
>Mature Secondary Structure 
ACGIAGIVLPEPGPVGAILTEMLDALQHRGPDSAGYGLYRDVDTAVFVLELPAEDGEME
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEEEECCCCCHHH
LLSEVERALEGRQLKRVERIAEDAGTRVYRLFVEGFSTGRQGQRELAEVVEKIENSDITV
HHHHHHHHHCCHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHCCCCEEE
LSAGHGFEILKDVGTAAEVSEQYGVEAIEGTHGIGHVRFSTESEVDRYHAHPFQSYMIPN
EECCCCHHHHHHCCCHHHHHHHHCCEEECCCCCCEEEEECCHHHHHHHHCCCHHHHCCCC
MAVVHNGQITNYYTIRERLEIKGYQFKTNNDSECIVVYVADKLRDGYSLEEAMEEAIRDL
EEEEECCCCCEEEEEEEHHEECCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHC
DGPFCFIISTPDAIAVARDPLGLRPGVIGFGKDGAVAVASEEVALRRIFGDELEGIEQIE
CCCEEEEEECCCEEEEECCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHCCHHHCC
PGEYEVFEVGGRG
CCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-phospho-beta-D-ribosylamine; diphosphate; L-glutamate

Specific reaction: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]