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The map label for this gene is rnz

Identifier: 20094469

GI number: 20094469

Start: 993238

End: 994077

Strand: Reverse

Name: rnz

Synonym: MK1033

Alternate gene names: 20094469

Gene position: 994077-993238 (Counterclockwise)

Preceding gene: 20094470

Following gene: 20094467

Centisome position: 58.65

GC content: 60.6

Gene sequence:

>840_bases
GTGGACCGTGAGCTGGTGATGCGCTTCCTGGGAACGGGTGGCGCGGTCCCGTCGAAGGATCGGAGCCACCCGGGACTGCT
GGTGGAGTTCTCCGGAACCAAGCTTCTAATCGACTGCGGAGAGGGTACCCAGCGGCGGGCCATGGAGCAAGGTGTCACGA
TCCACGATGTGGACGCTGTGCTGCTCACCCACCACCACGTCGATCACGTCGCGGGCCTCCTACCACTCGCGACCACCGTG
GACCTCCTCCACGGGCGTAGGTTGAAAGTCTACGGTCCCACGGCGGGTTCTGAGTCCGCTTTGGACATCTCCGACCTCGA
GGTGATCGAGTACCGGGAGGTGAATCCGGGAGACGAAGTAGAAATCGGTGACCTCAGGGTGCTGGTGTACGAGTCAGAAC
ACGGGGTTCCTACGGTCGACTATCGGATCGAGACACCGAAAATACCGGGAAAGGCGGATCCGAAGTACATACGAAGGGTC
CCTCCATCGAAGCGGCGTGAAGTGCTCTTACGCGGTGAGCGCCCGTACTCGTTGACGAAACCCGGTAAGATATCCGTGTA
TGTGAAAGGAGACGGTCGTCCGGCCGATCCTGAGAACGTCCGCGGTTGCCAGGTATTGGTACATGAAGCGTGCTTTGAGG
ATCATGAGGAAGCCGTGAGATACCTGCACTCGACCCACTTAGAGGCTGCCGAGGTCGCCCGCGAGGCAGGCGTCGATCTC
CTTGTACTGACGCACCTGTCCACGAAAGTGGATCCGGAAAGAATGAGGGAGGAGGCTCGGGAGGTGTTTCCAGTAGTCGT
CGTGGCGCGCGACGGACTCATGGTCCGTGTGCGGAGGTGA

Upstream 100 bases:

>100_bases
TGTAGGGTGAACTTCGAGACGATCGATGAACTGCTCTCGTTCCTGGAGGAGGGTATCGACCGCCTCGAGCTGGCGGTTTC
CGAGGTCCGGGGGACGGATC

Downstream 100 bases:

>100_bases
AGGCCCGTCAGCTACGGCCACACTCCGCATCGGTCCTCGGGGTCCCGGCTGTATCCTCATCCGGGGATCGCACAGTTGCG
CAGTTAGCCCACTAAACCGC

Product: beta-lactamase superfamily hydrolase

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MDRELVMRFLGTGGAVPSKDRSHPGLLVEFSGTKLLIDCGEGTQRRAMEQGVTIHDVDAVLLTHHHVDHVAGLLPLATTV
DLLHGRRLKVYGPTAGSESALDISDLEVIEYREVNPGDEVEIGDLRVLVYESEHGVPTVDYRIETPKIPGKADPKYIRRV
PPSKRREVLLRGERPYSLTKPGKISVYVKGDGRPADPENVRGCQVLVHEACFEDHEEAVRYLHSTHLEAAEVAREAGVDL
LVLTHLSTKVDPERMREEAREVFPVVVVARDGLMVRVRR

Sequences:

>Translated_279_residues
MDRELVMRFLGTGGAVPSKDRSHPGLLVEFSGTKLLIDCGEGTQRRAMEQGVTIHDVDAVLLTHHHVDHVAGLLPLATTV
DLLHGRRLKVYGPTAGSESALDISDLEVIEYREVNPGDEVEIGDLRVLVYESEHGVPTVDYRIETPKIPGKADPKYIRRV
PPSKRREVLLRGERPYSLTKPGKISVYVKGDGRPADPENVRGCQVLVHEACFEDHEEAVRYLHSTHLEAAEVAREAGVDL
LVLTHLSTKVDPERMREEAREVFPVVVVARDGLMVRVRR
>Mature_279_residues
MDRELVMRFLGTGGAVPSKDRSHPGLLVEFSGTKLLIDCGEGTQRRAMEQGVTIHDVDAVLLTHHHVDHVAGLLPLATTV
DLLHGRRLKVYGPTAGSESALDISDLEVIEYREVNPGDEVEIGDLRVLVYESEHGVPTVDYRIETPKIPGKADPKYIRRV
PPSKRREVLLRGERPYSLTKPGKISVYVKGDGRPADPENVRGCQVLVHEACFEDHEEAVRYLHSTHLEAAEVAREAGVDL
LVLTHLSTKVDPERMREEAREVFPVVVVARDGLMVRVRR

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Escherichia coli, GI87082078, Length=303, Percent_Identity=29.7029702970297, Blast_Score=103, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_METKA (Q8TWK0)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614316.1
- ProteinModelPortal:   Q8TWK0
- SMR:   Q8TWK0
- GeneID:   1477134
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1033
- NMPDR:   fig|190192.1.peg.1029
- HOGENOM:   HBG648406
- OMA:   LPCIALR
- BioCyc:   MKAN190192:MK1033-MONOMER
- BRENDA:   3.1.26.11
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 31074; Mature: 31074

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRELVMRFLGTGGAVPSKDRSHPGLLVEFSGTKLLIDCGEGTQRRAMEQGVTIHDVDAV
CCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHCCCEEEECCEE
LLTHHHVDHVAGLLPLATTVDLLHGRRLKVYGPTAGSESALDISDLEVIEYREVNPGDEV
EEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCE
EIGDLRVLVYESEHGVPTVDYRIETPKIPGKADPKYIRRVPPSKRREVLLRGERPYSLTK
EECCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHHEEEECCCCCCCCC
PGKISVYVKGDGRPADPENVRGCQVLVHEACFEDHEEAVRYLHSTHLEAAEVAREAGVDL
CCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCE
LVLTHLSTKVDPERMREEAREVFPVVVVARDGLMVRVRR
EEEECCCCCCCHHHHHHHHHHHCEEEEEECCCEEEEEEC
>Mature Secondary Structure
MDRELVMRFLGTGGAVPSKDRSHPGLLVEFSGTKLLIDCGEGTQRRAMEQGVTIHDVDAV
CCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHCCCEEEECCEE
LLTHHHVDHVAGLLPLATTVDLLHGRRLKVYGPTAGSESALDISDLEVIEYREVNPGDEV
EEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCE
EIGDLRVLVYESEHGVPTVDYRIETPKIPGKADPKYIRRVPPSKRREVLLRGERPYSLTK
EECCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCHHHHEEEECCCCCCCCC
PGKISVYVKGDGRPADPENVRGCQVLVHEACFEDHEEAVRYLHSTHLEAAEVAREAGVDL
CCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCE
LVLTHLSTKVDPERMREEAREVFPVVVVARDGLMVRVRR
EEEECCCCCCCHHHHHHHHHHHCEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014