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The map label for this gene is LysS

Identifier: 20094422

GI number: 20094422

Start: 945634

End: 947301

Strand: Direct

Name: LysS

Synonym: MK0986

Alternate gene names: 20094422

Gene position: 945634-947301 (Clockwise)

Preceding gene: 20094420

Following gene: 20094423

Centisome position: 55.79

GC content: 62.29

Gene sequence:

>1668_bases
TTGACCAAGGAACACTGGTCGGAGGTGAAGGCGCGGGAAGTTACCGAGCACTGTCGGAAGCACGCTGACGAACTCCCCGA
CGAGATAGTCGTGGCCAGCGGGGCTTCGACGTCCGGGCGCCTCCATGTGGGAAACGCCCGAGACGTTCTCACTGCAGACG
CCGTGGCCCGAGTCCTGCGAGAGCGCTACCATGAAGATGTTAGGGTCGTGTGGATCTCCGACGATGTGGATCCCTTACGT
CGCATCCCACGTGACCTCGACGGTAGACTCTCGGAGGATTACCTCGGTGTACCGTACAAGGCGATCCCAGTAGGCGACGA
GCCCTATTCTGACCGCTGGGCTCGTAACTTCGTGGAGGAGCTCCGGGAGTTCGGTGCGGAGGTTGAGTGGATCTCGTCGG
CCGAACTGTACACGGACAACGGCTTCGTAAAGCTGGTCCGAGAGGTCGTGAACGATTACTACGGCGGCGGTGGGAGGCTC
GCGAGCGTCCTCGAACGTTTCGGACTGGAGGATGCGCGGGTCTACATGCCGGTCTGCGAGGAGTGCGGCCGCATCGCTAC
TACTCGGGTGGTCGACGTAGACGGTTGGCGTATCGAGTACGTCTGCGAAGGACGGCATGAGATAGGGGACGCCGTCCTGG
AAGGATGTGGGCACCGTGGAGAGCTCGACCTCAGGAAACCGATAGAGGTCAATGGGTTCGAGATACCACCGGGCAAGCTC
GGGTGGAAGATCGAGTGGCCGACCCGCTGGGTCTACCTGGGTGTCGCCTGTGAACCCTTCGGCAAGGATCACTACGTGGC
CGGAGGCTCGTACGAGGTGGGGTCGGCTATCGCCGAGGAGTTCTTCGGGTTCCCGGCCCCGGTACCGGTCCCCTACGAGT
GGATCACGCTCGACGGCAAGGCGATGTCCTCCTCCAAGGGACACTACGTGACCCTGTCCGATTGGGGGGAAGTATGCCAC
CGCGAGGTGCTCCGCTACCTCGTCCTGCGCGGCAAACCGCTGAAACATCTCGACCTGGACCTACGGTTCGGTCTTCTGCA
GGCGGTGGACGACTACGATGAGTTAGAGAAGCGGTACTTCGCCGGTGAAGCCGACGAGAGGGAGCGGCGCATCTACGAGC
TTTCCCGAGTCGATGAGATCCCGGAGGAGTGTCCACCCCACGTACCGTTCCGGTTCTGCGCCGTCGTAGCTCAAGTAGTG
GGTATCGAAGACGACGTCTCGGAGGAAGAGTTCGAGCGGGCGCTGGAGATATTCCGGAGGACCGGACACCTTGAAGCGGA
GCCCGAGGGCTTCGGTCGGGAGTGGCTCCGGGAGAGACTCGAGAAGGCATCGCGATGGGTGGACAGGTACGCCCCGGAAG
AGGCCCGGTTCAGGGTTCGAGAGGAACCGGAACCCGTGGAGTTGTCAGACAAGGAGCGGGAGTTCCTCGAGGACCTGGTC
CGCCGGTTAGAATCGGAAACAACTAAAGAAGATCCGGAAACACTACAGCGGACGGTGTTCGAAGCCGCCCGTACCGCCGG
GCTACGTCCCGCGGACGCCTTCAGGGTGTTTTACAACGTCGTGGTCGGGAAGGACCGAGGTCCACGTGCTGGCACCCTCA
TCGCGGCTGTGGGGGTGGATCGGATTTCGAGGCTGATCCGGGGCTGCCTCGAGGCTTCCGAAGACTAG

Upstream 100 bases:

>100_bases
GCACGAGACCGGCCCCCGAGGTTACCGGCGGAAAAGGCGTCGCCACACCGCTCCGTTATTTATCGACCTCGAGCGGGCAA
GCCCACCGGGGAGCTGCTGC

Downstream 100 bases:

>100_bases
ACACCGGAAATGTGGTCCGTGGTGCCTTCCCGAAGGGTTGTAGGCTCTGCGTGTGCGGCGCCAAGCTGGTGGTGTTCGTG
ACCGGGGTCTGTAACCGCGG

Product: lysyl-tRNA synthetase (class I)

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTKEHWSEVKAREVTEHCRKHADELPDEIVVASGASTSGRLHVGNARDVLTADAVARVLRERYHEDVRVVWISDDVDPLR
RIPRDLDGRLSEDYLGVPYKAIPVGDEPYSDRWARNFVEELREFGAEVEWISSAELYTDNGFVKLVREVVNDYYGGGGRL
ASVLERFGLEDARVYMPVCEECGRIATTRVVDVDGWRIEYVCEGRHEIGDAVLEGCGHRGELDLRKPIEVNGFEIPPGKL
GWKIEWPTRWVYLGVACEPFGKDHYVAGGSYEVGSAIAEEFFGFPAPVPVPYEWITLDGKAMSSSKGHYVTLSDWGEVCH
REVLRYLVLRGKPLKHLDLDLRFGLLQAVDDYDELEKRYFAGEADERERRIYELSRVDEIPEECPPHVPFRFCAVVAQVV
GIEDDVSEEEFERALEIFRRTGHLEAEPEGFGREWLRERLEKASRWVDRYAPEEARFRVREEPEPVELSDKEREFLEDLV
RRLESETTKEDPETLQRTVFEAARTAGLRPADAFRVFYNVVVGKDRGPRAGTLIAAVGVDRISRLIRGCLEASED

Sequences:

>Translated_555_residues
MTKEHWSEVKAREVTEHCRKHADELPDEIVVASGASTSGRLHVGNARDVLTADAVARVLRERYHEDVRVVWISDDVDPLR
RIPRDLDGRLSEDYLGVPYKAIPVGDEPYSDRWARNFVEELREFGAEVEWISSAELYTDNGFVKLVREVVNDYYGGGGRL
ASVLERFGLEDARVYMPVCEECGRIATTRVVDVDGWRIEYVCEGRHEIGDAVLEGCGHRGELDLRKPIEVNGFEIPPGKL
GWKIEWPTRWVYLGVACEPFGKDHYVAGGSYEVGSAIAEEFFGFPAPVPVPYEWITLDGKAMSSSKGHYVTLSDWGEVCH
REVLRYLVLRGKPLKHLDLDLRFGLLQAVDDYDELEKRYFAGEADERERRIYELSRVDEIPEECPPHVPFRFCAVVAQVV
GIEDDVSEEEFERALEIFRRTGHLEAEPEGFGREWLRERLEKASRWVDRYAPEEARFRVREEPEPVELSDKEREFLEDLV
RRLESETTKEDPETLQRTVFEAARTAGLRPADAFRVFYNVVVGKDRGPRAGTLIAAVGVDRISRLIRGCLEASED
>Mature_554_residues
TKEHWSEVKAREVTEHCRKHADELPDEIVVASGASTSGRLHVGNARDVLTADAVARVLRERYHEDVRVVWISDDVDPLRR
IPRDLDGRLSEDYLGVPYKAIPVGDEPYSDRWARNFVEELREFGAEVEWISSAELYTDNGFVKLVREVVNDYYGGGGRLA
SVLERFGLEDARVYMPVCEECGRIATTRVVDVDGWRIEYVCEGRHEIGDAVLEGCGHRGELDLRKPIEVNGFEIPPGKLG
WKIEWPTRWVYLGVACEPFGKDHYVAGGSYEVGSAIAEEFFGFPAPVPVPYEWITLDGKAMSSSKGHYVTLSDWGEVCHR
EVLRYLVLRGKPLKHLDLDLRFGLLQAVDDYDELEKRYFAGEADERERRIYELSRVDEIPEECPPHVPFRFCAVVAQVVG
IEDDVSEEEFERALEIFRRTGHLEAEPEGFGREWLRERLEKASRWVDRYAPEEARFRVREEPEPVELSDKEREFLEDLVR
RLESETTKEDPETLQRTVFEAARTAGLRPADAFRVFYNVVVGKDRGPRAGTLIAAVGVDRISRLIRGCLEASED

Specific function: Unknown

COG id: COG1384

COG function: function code J; Lysyl-tRNA synthetase (class I)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_METKA (Q8TWP6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614269.1
- ProteinModelPortal:   Q8TWP6
- SMR:   Q8TWP6
- GeneID:   1477087
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0986
- NMPDR:   fig|190192.1.peg.982
- HOGENOM:   HBG297306
- OMA:   SKGNGLT
- BioCyc:   MKAN190192:MK0986-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00177
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR002904
- InterPro:   IPR020754
- InterPro:   IPR020756
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- TIGRFAMs:   TIGR00467

Pfam domain/function: PF01921 tRNA-synt_1f; SSF48163 tRNA-synt_bind

EC number: =6.1.1.6

Molecular weight: Translated: 63463; Mature: 63332

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEHWSEVKAREVTEHCRKHADELPDEIVVASGASTSGRLHVGNARDVLTADAVARVLR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHH
ERYHEDVRVVWISDDVDPLRRIPRDLDGRLSEDYLGVPYKAIPVGDEPYSDRWARNFVEE
HHCCCCEEEEEECCCCHHHHHCCHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
LREFGAEVEWISSAELYTDNGFVKLVREVVNDYYGGGGRLASVLERFGLEDARVYMPVCE
HHHHCCCEEEECCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
ECGRIATTRVVDVDGWRIEYVCEGRHEIGDAVLEGCGHRGELDLRKPIEVNGFEIPPGKL
HCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCC
GWKIEWPTRWVYLGVACEPFGKDHYVAGGSYEVGSAIAEEFFGFPAPVPVPYEWITLDGK
CEEEECCCEEEEEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCC
AMSSSKGHYVTLSDWGEVCHREVLRYLVLRGKPLKHLDLDLRFGLLQAVDDYDELEKRYF
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHH
AGEADERERRIYELSRVDEIPEECPPHVPFRFCAVVAQVVGIEDDVSEEEFERALEIFRR
CCCCCHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TGHLEAEPEGFGREWLRERLEKASRWVDRYAPEEARFRVREEPEPVELSDKEREFLEDLV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RRLESETTKEDPETLQRTVFEAARTAGLRPADAFRVFYNVVVGKDRGPRAGTLIAAVGVD
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RISRLIRGCLEASED
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKEHWSEVKAREVTEHCRKHADELPDEIVVASGASTSGRLHVGNARDVLTADAVARVLR
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHH
ERYHEDVRVVWISDDVDPLRRIPRDLDGRLSEDYLGVPYKAIPVGDEPYSDRWARNFVEE
HHCCCCEEEEEECCCCHHHHHCCHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
LREFGAEVEWISSAELYTDNGFVKLVREVVNDYYGGGGRLASVLERFGLEDARVYMPVCE
HHHHCCCEEEECCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
ECGRIATTRVVDVDGWRIEYVCEGRHEIGDAVLEGCGHRGELDLRKPIEVNGFEIPPGKL
HCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCC
GWKIEWPTRWVYLGVACEPFGKDHYVAGGSYEVGSAIAEEFFGFPAPVPVPYEWITLDGK
CEEEECCCEEEEEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCC
AMSSSKGHYVTLSDWGEVCHREVLRYLVLRGKPLKHLDLDLRFGLLQAVDDYDELEKRYF
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHH
AGEADERERRIYELSRVDEIPEECPPHVPFRFCAVVAQVVGIEDDVSEEEFERALEIFRR
CCCCCHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TGHLEAEPEGFGREWLRERLEKASRWVDRYAPEEARFRVREEPEPVELSDKEREFLEDLV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHH
RRLESETTKEDPETLQRTVFEAARTAGLRPADAFRVFYNVVVGKDRGPRAGTLIAAVGVD
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RISRLIRGCLEASED
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014