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The map label for this gene is nirB [C]

Identifier: 20094238

GI number: 20094238

Start: 762811

End: 763422

Strand: Direct

Name: nirB [C]

Synonym: MK0801

Alternate gene names: 20094238

Gene position: 762811-763422 (Clockwise)

Preceding gene: 20094237

Following gene: 20094240

Centisome position: 45.0

GC content: 60.95

Gene sequence:

>612_bases
GTGATTCAATCCCCTGACGGCACGTACGTGGTGAGGCTGGCACTTCCGCCGGGATTCGTCAAGGCTGAGATACTGAGGAC
CGTGGCGGAGCTCGCGGAACGGTACGCGAGCGGTGAAGTGCATATCACGGTGCGACAAGGTCTGGAGATCCCCGAGGTAC
CGCCGTCGAAGTTAGATGATCTCCTGCGTAAGCTCAGGGAGCTCGGCTTGGAGCCCGGATCGACGGGTCCTCGCGTTCGT
CAGGTGACCTGCTGTCCGGGCACCAGGACGTGTGCCAACGCACTCACGGACCCCGTACCATTGGCTCGGAAGTTGCACGA
AGAGTTCTTGGACGTGTGGGTGCCGGCCAAAGTCAAGATCGCCGTCTCGGGTTGCCCTCGGGGCTGCACGCGGCCGAGTG
AGAATGACCTGGGCCTGGTAGCAGTAGGTGGGGATGAATGGGAACTCCTGGTCGGGGGTTATAGAGTCGCTAGACTACCG
GAACAGGACGTCATCGATGCCGTCGAGTTGACGCTCGAATGGTACGCCTCGGAAGCCCCACCAGGGACGAGGTTGCACCG
GTTCGTTACGGATCGTGTTTCGGAACTTCGCGACGTGCTCGAGAGATTATGA

Upstream 100 bases:

>100_bases
TTGAGGAGGCGCGGAAAATAGTCCTTAGGGCGCTCGACGAATGGTACGAGGCAGTAAAGGATGCTTTGGAGCGTGCGGAT
GTTCCGAGGTCCGGCCCGGA

Downstream 100 bases:

>100_bases
GCCATAGGAACTGCCCGTACACCGGGAGCTTGAGGCCTATGTAGGCTCCTATCGATGCCGCGAGAAGCAACGGGACGATC
CAAACGGGCCTGCGTCGGTG

Product: oxidoreductase related to nitrite reductase

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIQSPDGTYVVRLALPPGFVKAEILRTVAELAERYASGEVHITVRQGLEIPEVPPSKLDDLLRKLRELGLEPGSTGPRVR
QVTCCPGTRTCANALTDPVPLARKLHEEFLDVWVPAKVKIAVSGCPRGCTRPSENDLGLVAVGGDEWELLVGGYRVARLP
EQDVIDAVELTLEWYASEAPPGTRLHRFVTDRVSELRDVLERL

Sequences:

>Translated_203_residues
MIQSPDGTYVVRLALPPGFVKAEILRTVAELAERYASGEVHITVRQGLEIPEVPPSKLDDLLRKLRELGLEPGSTGPRVR
QVTCCPGTRTCANALTDPVPLARKLHEEFLDVWVPAKVKIAVSGCPRGCTRPSENDLGLVAVGGDEWELLVGGYRVARLP
EQDVIDAVELTLEWYASEAPPGTRLHRFVTDRVSELRDVLERL
>Mature_203_residues
MIQSPDGTYVVRLALPPGFVKAEILRTVAELAERYASGEVHITVRQGLEIPEVPPSKLDDLLRKLRELGLEPGSTGPRVR
QVTCCPGTRTCANALTDPVPLARKLHEEFLDVWVPAKVKIAVSGCPRGCTRPSENDLGLVAVGGDEWELLVGGYRVARLP
EQDVIDAVELTLEWYASEAPPGTRLHRFVTDRVSELRDVLERL

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789765, Length=149, Percent_Identity=30.8724832214765, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 22367; Mature: 22367

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSPDGTYVVRLALPPGFVKAEILRTVAELAERYASGEVHITVRQGLEIPEVPPSKLDD
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHH
LLRKLRELGLEPGSTGPRVRQVTCCPGTRTCANALTDPVPLARKLHEEFLDVWVPAKVKI
HHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
AVSGCPRGCTRPSENDLGLVAVGGDEWELLVGGYRVARLPEQDVIDAVELTLEWYASEAP
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHCCCC
PGTRLHRFVTDRVSELRDVLERL
CCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIQSPDGTYVVRLALPPGFVKAEILRTVAELAERYASGEVHITVRQGLEIPEVPPSKLDD
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHH
LLRKLRELGLEPGSTGPRVRQVTCCPGTRTCANALTDPVPLARKLHEEFLDVWVPAKVKI
HHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
AVSGCPRGCTRPSENDLGLVAVGGDEWELLVGGYRVARLPEQDVIDAVELTLEWYASEAP
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHCCCC
PGTRLHRFVTDRVSELRDVLERL
CCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]