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The map label for this gene is LeuB_1 [H]

Identifier: 20094219

GI number: 20094219

Start: 743656

End: 744663

Strand: Direct

Name: LeuB_1 [H]

Synonym: MK0782

Alternate gene names: 20094219

Gene position: 743656-744663 (Clockwise)

Preceding gene: 20094218

Following gene: 20094220

Centisome position: 43.87

GC content: 61.9

Gene sequence:

>1008_bases
GTGGCGTACAAGATCGCTGTGATCCCGGGAGACGGCATCGGACCGGAAGTCATCGAGGCAGCCCTCCACGTGATCGAGCC
GCTGATCGACGCGGAGTTCGTCGAGGGTGAGGCCGGCGACGAATGCGCTGAGAAGCACGGCGATCCGCTGCCGGAGGATA
CCTTGGAGCTATGCCACGAAGCCGATGCGATCCTATTCGGGGCCGCGGGTGAGACGGCGGCCGACGTGATCGTCCGGCTG
AGACAGGAGCTCGACCTGTACGCGAACATCAGACCGGTACGTGGGTTTCCGGGACTCCGTGAGCTCACCGGTGAGCCGTA
CGTGCGGGACGACGTGGACTTCGTGATAGTGCGCGAGAATACCGAGGGGCTGTACTCAGGGATCGAGGGGAGATTCCGAG
ACACCGCGTACACCTTGAGGATCATCACAGAAGAAGGGACGCGGCGTATCGCCGAAGTAGCTTGCGATTTAGCGGAGGAG
CGCGGTTCGAATACCGTTACGTGTGTGCACAAGGCGAACGTCATGCGTGAGACGTGTGGGCTCTTCCGCGAAGTGTGCAA
GGAGGTGGTCGAATCCCGTGGACTGGAGTTCGAGGAGTACTACGTGGACGCTGCGGCTATGTTCATGATTACCGAGCCGG
AGCGCTTCGACGTCGTCGTGACACCGAACATGTTCGGCGACATACTATCTGACGAGGCGGCAGCACTCGTTGGTGGGCTG
GGGTTGGCGCCCAGCGGGAACGTCGGCGACCGCCATGGGCTGTTCGAACCGGTGCACGGATCGGCGCCGGACATCGCGGG
TAAAGGAATCGCGAACCCGTTCGCGACGATCCTTTCCGCGGTCATGATGCTAGAGTGGTTGGGTGAGGACGAAGCCGCGG
AAGCCGTAAGAGAGGCCGTGGGTGAGGCGATCCGAGAAGGTGTCGTCACGCCGGATCTAGGAGGTGATAAAAAGACGATG
GAGGTGGCGGAGTTCGTCAGGGAAGCTGCCCTCAACCGGGTCCAGTAG

Upstream 100 bases:

>100_bases
CGAAACCACTCCCGGACTTCATGATGAGGATACTCGAGGCCGGCGGCCTGGTGGAATTGATCAAGCGGGAAGGGCCACGG
GCTTTCGAGGGGTGACCTCC

Downstream 100 bases:

>100_bases
TGGGTCCAGTGGTGGACGATCCAGTAGTGGATTCTTCCTTCCCCGCACCCTGCTCCTCTCCGGATCGGCCTCCCACGTCC
TTAACTTTCGTCTTGGCGGC

Product: isocitrate/isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAYKIAVIPGDGIGPEVIEAALHVIEPLIDAEFVEGEAGDECAEKHGDPLPEDTLELCHEADAILFGAAGETAADVIVRL
RQELDLYANIRPVRGFPGLRELTGEPYVRDDVDFVIVRENTEGLYSGIEGRFRDTAYTLRIITEEGTRRIAEVACDLAEE
RGSNTVTCVHKANVMRETCGLFREVCKEVVESRGLEFEEYYVDAAAMFMITEPERFDVVVTPNMFGDILSDEAAALVGGL
GLAPSGNVGDRHGLFEPVHGSAPDIAGKGIANPFATILSAVMMLEWLGEDEAAEAVREAVGEAIREGVVTPDLGGDKKTM
EVAEFVREAALNRVQ

Sequences:

>Translated_335_residues
MAYKIAVIPGDGIGPEVIEAALHVIEPLIDAEFVEGEAGDECAEKHGDPLPEDTLELCHEADAILFGAAGETAADVIVRL
RQELDLYANIRPVRGFPGLRELTGEPYVRDDVDFVIVRENTEGLYSGIEGRFRDTAYTLRIITEEGTRRIAEVACDLAEE
RGSNTVTCVHKANVMRETCGLFREVCKEVVESRGLEFEEYYVDAAAMFMITEPERFDVVVTPNMFGDILSDEAAALVGGL
GLAPSGNVGDRHGLFEPVHGSAPDIAGKGIANPFATILSAVMMLEWLGEDEAAEAVREAVGEAIREGVVTPDLGGDKKTM
EVAEFVREAALNRVQ
>Mature_334_residues
AYKIAVIPGDGIGPEVIEAALHVIEPLIDAEFVEGEAGDECAEKHGDPLPEDTLELCHEADAILFGAAGETAADVIVRLR
QELDLYANIRPVRGFPGLRELTGEPYVRDDVDFVIVRENTEGLYSGIEGRFRDTAYTLRIITEEGTRRIAEVACDLAEER
GSNTVTCVHKANVMRETCGLFREVCKEVVESRGLEFEEYYVDAAAMFMITEPERFDVVVTPNMFGDILSDEAAALVGGLG
LAPSGNVGDRHGLFEPVHGSAPDIAGKGIANPFATILSAVMMLEWLGEDEAAEAVREAVGEAIREGVVTPDLGGDKKTME
VAEFVREAALNRVQ

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=338, Percent_Identity=40.5325443786982, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=36.0119047619048, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI28178838, Length=315, Percent_Identity=35.8730158730159, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI28178821, Length=338, Percent_Identity=34.0236686390533, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI28178816, Length=338, Percent_Identity=34.0236686390533, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI28178819, Length=192, Percent_Identity=38.0208333333333, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1788101, Length=352, Percent_Identity=38.3522727272727, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081683, Length=340, Percent_Identity=38.5294117647059, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787381, Length=384, Percent_Identity=31.5104166666667, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71986051, Length=327, Percent_Identity=40.3669724770642, Blast_Score=224, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17550882, Length=336, Percent_Identity=36.0119047619048, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17505779, Length=330, Percent_Identity=35.1515151515151, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=31.2865497076023, Blast_Score=167, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=45.7142857142857, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6324291, Length=335, Percent_Identity=35.8208955223881, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319830, Length=356, Percent_Identity=34.5505617977528, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=34.0175953079179, Blast_Score=166, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24643270, Length=335, Percent_Identity=42.6865671641791, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24643268, Length=335, Percent_Identity=42.6865671641791, Blast_Score=234, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24661184, Length=341, Percent_Identity=39.0029325513196, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI161078637, Length=337, Percent_Identity=31.1572700296736, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=31.1572700296736, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI161078633, Length=337, Percent_Identity=31.1572700296736, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24650122, Length=337, Percent_Identity=31.1572700296736, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI161078639, Length=253, Percent_Identity=35.1778656126482, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI281362242, Length=344, Percent_Identity=32.8488372093023, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24648872, Length=344, Percent_Identity=32.8488372093023, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI20130355, Length=329, Percent_Identity=30.0911854103343, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45550436, Length=255, Percent_Identity=22.3529411764706, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36282; Mature: 36151

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKIAVIPGDGIGPEVIEAALHVIEPLIDAEFVEGEAGDECAEKHGDPLPEDTLELCHE
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHH
ADAILFGAAGETAADVIVRLRQELDLYANIRPVRGFPGLRELTGEPYVRDDVDFVIVREN
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEECCCCEEEEECC
TEGLYSGIEGRFRDTAYTLRIITEEGTRRIAEVACDLAEERGSNTVTCVHKANVMRETCG
CCHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
LFREVCKEVVESRGLEFEEYYVDAAAMFMITEPERFDVVVTPNMFGDILSDEAAALVGGL
HHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHCC
GLAPSGNVGDRHGLFEPVHGSAPDIAGKGIANPFATILSAVMMLEWLGEDEAAEAVREAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GEAIREGVVTPDLGGDKKTMEVAEFVREAALNRVQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AYKIAVIPGDGIGPEVIEAALHVIEPLIDAEFVEGEAGDECAEKHGDPLPEDTLELCHE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHH
ADAILFGAAGETAADVIVRLRQELDLYANIRPVRGFPGLRELTGEPYVRDDVDFVIVREN
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEECCCCEEEEECC
TEGLYSGIEGRFRDTAYTLRIITEEGTRRIAEVACDLAEERGSNTVTCVHKANVMRETCG
CCHHHHCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
LFREVCKEVVESRGLEFEEYYVDAAAMFMITEPERFDVVVTPNMFGDILSDEAAALVGGL
HHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHCC
GLAPSGNVGDRHGLFEPVHGSAPDIAGKGIANPFATILSAVMMLEWLGEDEAAEAVREAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GEAIREGVVTPDLGGDKKTMEVAEFVREAALNRVQ
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]