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The map label for this gene is LeuD_1

Identifier: 20094218

GI number: 20094218

Start: 743138

End: 743650

Strand: Direct

Name: LeuD_1

Synonym: MK0781

Alternate gene names: 20094218

Gene position: 743138-743650 (Clockwise)

Preceding gene: 20094217

Following gene: 20094219

Centisome position: 43.84

GC content: 63.16

Gene sequence:

>513_bases
TTGAGAGACGTTATCCGGGGAAGGGCCTGGGTCTTCGGGGACGATATCGACACCGACCAGATCATCCCGGGTCGATACCT
CACTACCCAGGACCCCGAGGAGCTGGCCAAGCACGTGATGGAAGGGGCCGATCCGGAGTTCCCCGAGAAGGTCCGCGAGG
GCGACGTGATCGTGGCCGGGAAGAACTTCGGCTGCGGTTCCTCCCGTGAGCACGCGCCGATCGCGCTCAAAGCTGCCGGA
ATCGCCTGCGTGGTCACGAGGTCCTTCGCCCGTATCTTCTACCGGAACGCGATCAACCTGGGGTTACCCCTAGTGGTTTG
CCCCGGCGTGGACGACGCGTTCGAGGATGGCCAGGGGATCGAGGTGAATCTCCGAGAGGGGTATGTCAGGAACCTCGACA
CCGGTGAGGAGCTGGAGGCGAAACCACTCCCGGACTTCATGATGAGGATACTCGAGGCCGGCGGCCTGGTGGAATTGATC
AAGCGGGAAGGGCCACGGGCTTTCGAGGGGTGA

Upstream 100 bases:

>100_bases
CGTATCCGCGTGCCTGACGATCTTCAGAGAGCCGGTGTTCGACTGATCGGTCGACAAAGGTGATAACTCGCCGGTCACGG
GGGCGGCCCGGGGCGTCCGC

Downstream 100 bases:

>100_bases
CCTCCGTGGCGTACAAGATCGCTGTGATCCCGGGAGACGGCATCGGACCGGAAGTCATCGAGGCAGCCCTCCACGTGATC
GAGCCGCTGATCGACGCGGA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MRDVIRGRAWVFGDDIDTDQIIPGRYLTTQDPEELAKHVMEGADPEFPEKVREGDVIVAGKNFGCGSSREHAPIALKAAG
IACVVTRSFARIFYRNAINLGLPLVVCPGVDDAFEDGQGIEVNLREGYVRNLDTGEELEAKPLPDFMMRILEAGGLVELI
KREGPRAFEG

Sequences:

>Translated_170_residues
MRDVIRGRAWVFGDDIDTDQIIPGRYLTTQDPEELAKHVMEGADPEFPEKVREGDVIVAGKNFGCGSSREHAPIALKAAG
IACVVTRSFARIFYRNAINLGLPLVVCPGVDDAFEDGQGIEVNLREGYVRNLDTGEELEAKPLPDFMMRILEAGGLVELI
KREGPRAFEG
>Mature_170_residues
MRDVIRGRAWVFGDDIDTDQIIPGRYLTTQDPEELAKHVMEGADPEFPEKVREGDVIVAGKNFGCGSSREHAPIALKAAG
IACVVTRSFARIFYRNAINLGLPLVVCPGVDDAFEDGQGIEVNLREGYVRNLDTGEELEAKPLPDFMMRILEAGGLVELI
KREGPRAFEG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=99, Percent_Identity=37.3737373737374, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320440, Length=130, Percent_Identity=42.3076923076923, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=40.7766990291262, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD1_METKA (Q8TX94)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614065.1
- ProteinModelPortal:   Q8TX94
- SMR:   Q8TX94
- GeneID:   1476882
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0781
- NMPDR:   fig|190192.1.peg.778
- HOGENOM:   HBG304838
- OMA:   HAFESID
- BioCyc:   MKAN190192:MK0781-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18687; Mature: 18687

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDVIRGRAWVFGDDIDTDQIIPGRYLTTQDPEELAKHVMEGADPEFPEKVREGDVIVAG
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEEC
KNFGCGSSREHAPIALKAAGIACVVTRSFARIFYRNAINLGLPLVVCPGVDDAFEDGQGI
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCE
EVNLREGYVRNLDTGEELEAKPLPDFMMRILEAGGLVELIKREGPRAFEG
EEEECCCEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRDVIRGRAWVFGDDIDTDQIIPGRYLTTQDPEELAKHVMEGADPEFPEKVREGDVIVAG
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCEEEEC
KNFGCGSSREHAPIALKAAGIACVVTRSFARIFYRNAINLGLPLVVCPGVDDAFEDGQGI
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHCCCCCE
EVNLREGYVRNLDTGEELEAKPLPDFMMRILEAGGLVELIKREGPRAFEG
EEEECCCEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014