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The map label for this gene is DPH5

Identifier: 20094184

GI number: 20094184

Start: 708436

End: 709227

Strand: Direct

Name: DPH5

Synonym: MK0747

Alternate gene names: 20094184

Gene position: 708436-709227 (Clockwise)

Preceding gene: 20094183

Following gene: 20094185

Centisome position: 41.8

GC content: 62.12

Gene sequence:

>792_bases
ATGGGTGAGCTGTACCTGGTCGGAGGGGGTCTCTCCGACGTTCGAGATCTCACGCTGCGGGCCCTTGAAGTGCTAGCTAG
CGTGGAGTTGGTGCTGGTCGACACCTACACCAGCGTGTACGACGTAAGCGAGGGCGACCTGAAACGGTTGCTCAACGGGT
TCGGAGGTGATCCTGAAGTACGTATGTGCTCGCGGCGGGACCTGGAGGAGCGCTTCTTCGACCTGTGTGAGGGGTACGAT
CGCGTCGCCCTGCTCTCCCCGGGCGACCCGATGGCAGCGACCACTCACGTGGCGCTGGTGGTCGAGGCCGCCGACCGTGG
GTGGGACGTCGAGATAATCAACGGCGTATCGGTGTTCACCGCGGCACCATCCAAGTCGGGGCTGGAAATGTACCGGTTCG
GACGTACCGCCACGATTCCGTTGAACGTGCGCTCCGTGTATCCCTACGATGTGCTCGAATCGAACCGGCAGGCCGGTTTA
CACACCCTCTTCCTGCTGGAAGTTGCTGAAGACGGGGAGTTCGTCTCTGTCGCTGACGCCGCCCGCTACCTATTGGAAAT
TGAGAGGGAAGAAGGTCGCGGAGTACTCGACCCCTCGGATCTCGCGATCGCCGTAGTGAGGCTGGGGTTCGAGGACGAGT
TGGTGGCTTGGGGAACCCTCGAAGAACTCTCCGACTGGGAGCCCGGTGAACCTCCGCAAGCTCTAATACTGCCCGCCTCC
CGACTCCGTGAAGCGGAGCGCGAATACATCCGCCGCGTCCTCCCACACATCCGAGATGTGCGTGGGGTCTAG

Upstream 100 bases:

>100_bases
TTCGAAGAAGACGGCTCTTGCGGCCACGTACTCAAGATGCGGGGCTCGCCTGAGGACGTCGTCCGTGAGGCGGTGTCTCA
GGCTAGGGAGGTGTTATCGG

Downstream 100 bases:

>100_bases
CCTTTGGGTGAGGAGACTGGCGGTGACTATCACGTCGTGCTCGAGGCCGCGTGGATCGTGTACAACGTCGACGACGAGGA
CGACGCTATCAACATCGCCA

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGELYLVGGGLSDVRDLTLRALEVLASVELVLVDTYTSVYDVSEGDLKRLLNGFGGDPEVRMCSRRDLEERFFDLCEGYD
RVALLSPGDPMAATTHVALVVEAADRGWDVEIINGVSVFTAAPSKSGLEMYRFGRTATIPLNVRSVYPYDVLESNRQAGL
HTLFLLEVAEDGEFVSVADAARYLLEIEREEGRGVLDPSDLAIAVVRLGFEDELVAWGTLEELSDWEPGEPPQALILPAS
RLREAEREYIRRVLPHIRDVRGV

Sequences:

>Translated_263_residues
MGELYLVGGGLSDVRDLTLRALEVLASVELVLVDTYTSVYDVSEGDLKRLLNGFGGDPEVRMCSRRDLEERFFDLCEGYD
RVALLSPGDPMAATTHVALVVEAADRGWDVEIINGVSVFTAAPSKSGLEMYRFGRTATIPLNVRSVYPYDVLESNRQAGL
HTLFLLEVAEDGEFVSVADAARYLLEIEREEGRGVLDPSDLAIAVVRLGFEDELVAWGTLEELSDWEPGEPPQALILPAS
RLREAEREYIRRVLPHIRDVRGV
>Mature_262_residues
GELYLVGGGLSDVRDLTLRALEVLASVELVLVDTYTSVYDVSEGDLKRLLNGFGGDPEVRMCSRRDLEERFFDLCEGYDR
VALLSPGDPMAATTHVALVVEAADRGWDVEIINGVSVFTAAPSKSGLEMYRFGRTATIPLNVRSVYPYDVLESNRQAGLH
TLFLLEVAEDGEFVSVADAARYLLEIEREEGRGVLDPSDLAIAVVRLGFEDELVAWGTLEELSDWEPGEPPQALILPASR
LREAEREYIRRVLPHIRDVRGV

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190324, Length=271, Percent_Identity=32.1033210332103, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI117190326, Length=272, Percent_Identity=31.9852941176471, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI117190262, Length=272, Percent_Identity=31.9852941176471, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531369, Length=261, Percent_Identity=31.0344827586207, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323201, Length=255, Percent_Identity=32.9411764705882, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28572120, Length=281, Percent_Identity=31.3167259786477, Blast_Score=127, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_METKA (Q8TXC7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614031.1
- ProteinModelPortal:   Q8TXC7
- SMR:   Q8TXC7
- GeneID:   1476848
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0747
- NMPDR:   fig|190192.1.peg.744
- HOGENOM:   HBG460511
- OMA:   GLEMYRF
- ProtClustDB:   PRK04160
- BioCyc:   MKAN190192:MK0747-MONOMER
- BRENDA:   2.1.1.98
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 29155; Mature: 29024

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELYLVGGGLSDVRDLTLRALEVLASVELVLVDTYTSVYDVSEGDLKRLLNGFGGDPEV
CCEEEEECCCHHHHHHHHHHHHHHHHHCEEEEEECCHHEEECCHHHHHHHHHHCCCCCCC
RMCSRRDLEERFFDLCEGYDRVALLSPGDPMAATTHVALVVEAADRGWDVEIINGVSVFT
HHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCHHEEEEEEEEECCCCCCEEEECCEEEEE
AAPSKSGLEMYRFGRTATIPLNVRSVYPYDVLESNRQAGLHTLFLLEVAEDGEFVSVADA
ECCCCCCCHHHHCCCEEEEEEEECEECCHHHHHCCCCCCCEEEEEEEECCCCCEEEHHHH
ARYLLEIEREEGRGVLDPSDLAIAVVRLGFEDELVAWGTLEELSDWEPGEPPQALILPAS
HHHHHHEEHHCCCCCCCCHHHEEEEEEECCCCCEEECCCHHHHCCCCCCCCCCEEEECHH
RLREAEREYIRRVLPHIRDVRGV
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GELYLVGGGLSDVRDLTLRALEVLASVELVLVDTYTSVYDVSEGDLKRLLNGFGGDPEV
CEEEEECCCHHHHHHHHHHHHHHHHHCEEEEEECCHHEEECCHHHHHHHHHHCCCCCCC
RMCSRRDLEERFFDLCEGYDRVALLSPGDPMAATTHVALVVEAADRGWDVEIINGVSVFT
HHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCHHEEEEEEEEECCCCCCEEEECCEEEEE
AAPSKSGLEMYRFGRTATIPLNVRSVYPYDVLESNRQAGLHTLFLLEVAEDGEFVSVADA
ECCCCCCCHHHHCCCEEEEEEEECEECCHHHHHCCCCCCCEEEEEEEECCCCCEEEHHHH
ARYLLEIEREEGRGVLDPSDLAIAVVRLGFEDELVAWGTLEELSDWEPGEPPQALILPAS
HHHHHHEEHHCCCCCCCCHHHEEEEEEECCCCCEEECCCHHHHCCCCCCCCCCEEEECHH
RLREAEREYIRRVLPHIRDVRGV
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014