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The map label for this gene is rsmF [H]

Identifier: 20094111

GI number: 20094111

Start: 630694

End: 631617

Strand: Direct

Name: rsmF [H]

Synonym: MK0673

Alternate gene names: 20094111

Gene position: 630694-631617 (Clockwise)

Preceding gene: 20094106

Following gene: 20094112

Centisome position: 37.21

GC content: 64.72

Gene sequence:

>924_bases
ATGAGGTACGTGGAAGAGTTCCGTGAGCGTTTGGAGCGGATCTTCGGTGACCGTGCCGAGGACGTGTACCGGTACCTGTT
GGAGGGTCGTCCACCCACTTACGTCCGGGTGAACACGCTGAAGGCGGACGTCGAGGAGGTTGTGGAGAACCTCGAAACGG
CGGGAGTCGAGCTCGCCGAAACCCCACTATCGTACGCCTTCCGTGTCCTCAGTAGTCCCGGGCCGCTGGGTTCCTCGCTG
GAACACGTGGCCGGTTACCTTTACCTTCAGGACCTGGCTTCGATGGTCCCCCCGGAGCTCCTTGACCCGGAACCTCCGGG
TCCCGTGATAGACCTGTGCGCGGCCCCCGGGAGCAAAACCACGCAGTTAGCACAGCTGCTCGGCGGCGAGGGCGTCGTAC
TGGCCGTCGACGCCGACCCTCGGCGCGTCCGGGCTCTGGTTCACAACGTGAACAGGCTGGGATGTGTCAACGTGATCGTG
GCACACGCCGACGCGGCACGTCTGCGTATCAGCGCCCCTTTCCTCCTGCTGGATCCCCCGTGCTCGGGTGAGGGCACCCT
GCACCGAGATCCACACGCCCTACGTACCTGGACTCCGAAGAAACCCGGGAGGTTCGCCCGGACGCAGCTGCGCCTCCTGA
GGGCCGCCCTGAGGATGCTGCCGCCGGGCGGCCGTCTGGTGTACTCGACCTGTACGTTCTCCGTCGAAGAGAACGAGCTG
GTGATCCACGAGGCTCTAGGGAACGACGACAGGTACCGGATAGTCCCGTCCGTCCCACGGTGGCTCGAACCTCACACCGT
CCCCGGGCTAACCGAGTGGGAAGGGAGGGAGCTCCGGGAGGACCTGAGGCACGCCTTCAGGATCGATCCGGCCAGCCTAG
AATCCGACGGTTTTTTCGTCGCGGTAATCGAGCGTCGCCACTGA

Upstream 100 bases:

>100_bases
CGTCGGGAACGTGGTCCTGGGTGTGGGGAAAGTCGGTCGTGACGGGAAACTCCGATGCCGTGCACCGAGGAAGTACGCGA
GACTCAGGTTGTGGTGAGCG

Downstream 100 bases:

>100_bases
CAATTTAGTGTACGACCGTCACCCTCCGAGTGGGACTTAATCACGTCCACCTCCACTCTCTGCGGGTGATCACGTGGCAG
GAGACAACGAAGCGACCGAA

Product: tRNA/rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRYVEEFRERLERIFGDRAEDVYRYLLEGRPPTYVRVNTLKADVEEVVENLETAGVELAETPLSYAFRVLSSPGPLGSSL
EHVAGYLYLQDLASMVPPELLDPEPPGPVIDLCAAPGSKTTQLAQLLGGEGVVLAVDADPRRVRALVHNVNRLGCVNVIV
AHADAARLRISAPFLLLDPPCSGEGTLHRDPHALRTWTPKKPGRFARTQLRLLRAALRMLPPGGRLVYSTCTFSVEENEL
VIHEALGNDDRYRIVPSVPRWLEPHTVPGLTEWEGRELREDLRHAFRIDPASLESDGFFVAVIERRH

Sequences:

>Translated_307_residues
MRYVEEFRERLERIFGDRAEDVYRYLLEGRPPTYVRVNTLKADVEEVVENLETAGVELAETPLSYAFRVLSSPGPLGSSL
EHVAGYLYLQDLASMVPPELLDPEPPGPVIDLCAAPGSKTTQLAQLLGGEGVVLAVDADPRRVRALVHNVNRLGCVNVIV
AHADAARLRISAPFLLLDPPCSGEGTLHRDPHALRTWTPKKPGRFARTQLRLLRAALRMLPPGGRLVYSTCTFSVEENEL
VIHEALGNDDRYRIVPSVPRWLEPHTVPGLTEWEGRELREDLRHAFRIDPASLESDGFFVAVIERRH
>Mature_307_residues
MRYVEEFRERLERIFGDRAEDVYRYLLEGRPPTYVRVNTLKADVEEVVENLETAGVELAETPLSYAFRVLSSPGPLGSSL
EHVAGYLYLQDLASMVPPELLDPEPPGPVIDLCAAPGSKTTQLAQLLGGEGVVLAVDADPRRVRALVHNVNRLGCVNVIV
AHADAARLRISAPFLLLDPPCSGEGTLHRDPHALRTWTPKKPGRFARTQLRLLRAALRMLPPGGRLVYSTCTFSVEENEL
VIHEALGNDDRYRIVPSVPRWLEPHTVPGLTEWEGRELREDLRHAFRIDPASLESDGFFVAVIERRH

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=285, Percent_Identity=35.7894736842105, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI76150623, Length=285, Percent_Identity=35.7894736842105, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI270288816, Length=302, Percent_Identity=37.0860927152318, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI8922322, Length=302, Percent_Identity=37.0860927152318, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI270288818, Length=302, Percent_Identity=37.0860927152318, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI23199998, Length=302, Percent_Identity=37.0860927152318, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI39995082, Length=220, Percent_Identity=34.5454545454545, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI301336155, Length=220, Percent_Identity=34.0909090909091, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI32698918, Length=251, Percent_Identity=33.8645418326693, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI11545785, Length=214, Percent_Identity=32.2429906542056, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI87081985, Length=285, Percent_Identity=37.1929824561403, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI2367212, Length=244, Percent_Identity=29.0983606557377, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=284, Percent_Identity=33.4507042253521, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25143471, Length=382, Percent_Identity=28.2722513089005, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71998419, Length=307, Percent_Identity=31.2703583061889, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115534021, Length=231, Percent_Identity=34.1991341991342, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324268, Length=286, Percent_Identity=32.8671328671329, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319447, Length=271, Percent_Identity=31.7343173431734, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI22024126, Length=285, Percent_Identity=33.6842105263158, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21355201, Length=284, Percent_Identity=31.3380281690141, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24668781, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356513, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356579, Length=308, Percent_Identity=25, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920494, Length=170, Percent_Identity=30.5882352941176, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 34315; Mature: 34315

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYVEEFRERLERIFGDRAEDVYRYLLEGRPPTYVRVNTLKADVEEVVENLETAGVELAE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHH
TPLSYAFRVLSSPGPLGSSLEHVAGYLYLQDLASMVPPELLDPEPPGPVIDLCAAPGSKT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCHH
TQLAQLLGGEGVVLAVDADPRRVRALVHNVNRLGCVNVIVAHADAARLRISAPFLLLDPP
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCEEEECCC
CSGEGTLHRDPHALRTWTPKKPGRFARTQLRLLRAALRMLPPGGRLVYSTCTFSVEENEL
CCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCE
VIHEALGNDDRYRIVPSVPRWLEPHTVPGLTEWEGRELREDLRHAFRIDPASLESDGFFV
EEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE
AVIERRH
EEEECCC
>Mature Secondary Structure
MRYVEEFRERLERIFGDRAEDVYRYLLEGRPPTYVRVNTLKADVEEVVENLETAGVELAE
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHH
TPLSYAFRVLSSPGPLGSSLEHVAGYLYLQDLASMVPPELLDPEPPGPVIDLCAAPGSKT
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCHH
TQLAQLLGGEGVVLAVDADPRRVRALVHNVNRLGCVNVIVAHADAARLRISAPFLLLDPP
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCEEEECCC
CSGEGTLHRDPHALRTWTPKKPGRFARTQLRLLRAALRMLPPGGRLVYSTCTFSVEENEL
CCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCE
VIHEALGNDDRYRIVPSVPRWLEPHTVPGLTEWEGRELREDLRHAFRIDPASLESDGFFV
EEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEE
AVIERRH
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA