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The map label for this gene is frhB [H]

Identifier: 20093977

GI number: 20093977

Start: 503796

End: 504623

Strand: Direct

Name: frhB [H]

Synonym: MK0539

Alternate gene names: 20093977

Gene position: 503796-504623 (Clockwise)

Preceding gene: 20093976

Following gene: 20093978

Centisome position: 29.72

GC content: 58.7

Gene sequence:

>828_bases
GTGGGTGAGTACCGCGAGGTTTACCTGACCCGCGCAACCGACGAACGTATCCGCGAGCACGGACAGGACGGAGGTACTAC
TACGGCGCTACTCGCCCACGCACTGGAGGAGGACACGGTGGAAGCCGTGATTGCCTCGTCGACAGTCGAGACATGGAAGC
CCGAACCCGTAATAGTCACCGATCCCGACGAACTGATCGAGACCGCGGGATCGAAGTACGCGATTTCTCCGAACGTGAGT
GCCCTCAACGAGGCCATAGCCTCCTACGACTCAGTCGCCCTCGTGGGCACGCCGTGTCAGATCACCGCCGTAAAGAAGTC
TAAAATGTACCCTTACGGCCTGGCTAACGTGACGGAGCGTGTGAAGCTAACCGTCGGCATCTTCTGCACCGAGAACTTCC
AGTACGAGAGCCTACTGAAGCTGCTCGAAGACATGGGTGTCGACGTCGAGAACGTCGAGCGTATGGACATCAGCCACGGT
GAATTCATCGTACGCACTAAGAACGGTGATGTGCACTCGGTATCGGTTTCCAAGCTAGGCGATTACGCCAACGAGGCGTG
TAACTACTGCACGGACTTCACGGCCGAGGACGCCGACATCAGCGTGGGATCCGTCGGCGCGCCGGATGGCTGGAACGTGG
TTCTGGTCCGGACGAAAGAAGGCGAGAAGGTGTTCCGGTCCGCCGTAGACGCTGACGTCCTGGAAGTCAAAGATATCGGG
GAAGGGGACCCGAACCTGCTCGAGCGCCTGGCCAGGGACAAGAAGGAACGCATTCACACCTCCATGTGTGCCACTTGGAG
ACCTTATCACCCTACCGTTCCTCTCTAA

Upstream 100 bases:

>100_bases
ATCCGTGTTGCGCAAGCTGATCCGAAGAATAGCAGGGGGCAAAGAGGAAGAGAATACCCGTGAGGGATCTCCCGAGAACG
ATCTCCCGGACCCCGAGGTC

Downstream 100 bases:

>100_bases
CGTCATTTCACTATTTCTCCGAGTTCAAGGGGCCCAGAGCTGTGATCAAGCCACGAGATTTCCTGAGGTATTTCCTCTAC
TCTTACATCGTAACTTCCGC

Product: coenzyme F420-reducing hydrogenase subunit beta

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta; FRH [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MGEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVETWKPEPVIVTDPDELIETAGSKYAISPNVS
ALNEAIASYDSVALVGTPCQITAVKKSKMYPYGLANVTERVKLTVGIFCTENFQYESLLKLLEDMGVDVENVERMDISHG
EFIVRTKNGDVHSVSVSKLGDYANEACNYCTDFTAEDADISVGSVGAPDGWNVVLVRTKEGEKVFRSAVDADVLEVKDIG
EGDPNLLERLARDKKERIHTSMCATWRPYHPTVPL

Sequences:

>Translated_275_residues
MGEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVETWKPEPVIVTDPDELIETAGSKYAISPNVS
ALNEAIASYDSVALVGTPCQITAVKKSKMYPYGLANVTERVKLTVGIFCTENFQYESLLKLLEDMGVDVENVERMDISHG
EFIVRTKNGDVHSVSVSKLGDYANEACNYCTDFTAEDADISVGSVGAPDGWNVVLVRTKEGEKVFRSAVDADVLEVKDIG
EGDPNLLERLARDKKERIHTSMCATWRPYHPTVPL
>Mature_274_residues
GEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVETWKPEPVIVTDPDELIETAGSKYAISPNVSA
LNEAIASYDSVALVGTPCQITAVKKSKMYPYGLANVTERVKLTVGIFCTENFQYESLLKLLEDMGVDVENVERMDISHGE
FIVRTKNGDVHSVSVSKLGDYANEACNYCTDFTAEDADISVGSVGAPDGWNVVLVRTKEGEKVFRSAVDADVLEVKDIGE
GDPNLLERLARDKKERIHTSMCATWRPYHPTVPL

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the frhB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007516
- InterPro:   IPR017679
- InterPro:   IPR007525 [H]

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 30220; Mature: 30089

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVETWKPEPVIVT
CCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEE
DPDELIETAGSKYAISPNVSALNEAIASYDSVALVGTPCQITAVKKSKMYPYGLANVTER
CHHHHHHHCCCCEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHE
VKLTVGIFCTENFQYESLLKLLEDMGVDVENVERMDISHGEFIVRTKNGDVHSVSVSKLG
EEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHEECCCCCEEEEECCCCEEEEEHHHHH
DYANEACNYCTDFTAEDADISVGSVGAPDGWNVVLVRTKEGEKVFRSAVDADVLEVKDIG
HHHHHHHHHHCCCCCCCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHCCHHEEHHCCC
EGDPNLLERLARDKKERIHTSMCATWRPYHPTVPL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVETWKPEPVIVT
CCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEE
DPDELIETAGSKYAISPNVSALNEAIASYDSVALVGTPCQITAVKKSKMYPYGLANVTER
CHHHHHHHCCCCEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCCCHHHHHHE
VKLTVGIFCTENFQYESLLKLLEDMGVDVENVERMDISHGEFIVRTKNGDVHSVSVSKLG
EEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHEECCCCCEEEEECCCCEEEEEHHHHH
DYANEACNYCTDFTAEDADISVGSVGAPDGWNVVLVRTKEGEKVFRSAVDADVLEVKDIG
HHHHHHHHHHCCCCCCCCCCEECCCCCCCCCEEEEEEECCCHHHHHHHHCCHHEEHHCCC
EGDPNLLERLARDKKERIHTSMCATWRPYHPTVPL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2207102; 9371463; 3663585 [H]