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The map label for this gene is nuoB [C]

Identifier: 20093976

GI number: 20093976

Start: 502964

End: 503680

Strand: Direct

Name: nuoB [C]

Synonym: MK0538

Alternate gene names: 20093976

Gene position: 502964-503680 (Clockwise)

Preceding gene: 20093975

Following gene: 20093977

Centisome position: 29.67

GC content: 60.53

Gene sequence:

>717_bases
ATGAAGGTGGCTGTGCTGTCCCTTATGGCTTGTGGTACATGCGCCAGGAACCTGGAGGAGGTTTGCCACGAAGAGGGTCA
TGAGGTGGTCTGGTCGAACCTAGAAGGCGACGAGGGAGAACCGGACGCCGACATTCTGTTCGTAGAAGGCGCGGTGGACT
TCGCCGACGCCGAGACCCTGGAAAAGTTAGAGGAGGCCGCTTCGGCCGTCAGTACCGTGGTATCACTGGGTTCCTGTGCC
GCTACCGGGGGTTTCGAGCGACACGTCATAGGCATGCGCGACCCCGACCGGTACCACTTCACGGTCTTCCCAGCTGCCAA
GTTCGTCGAAACGGACTATGCGATCAACGGCTGTCCACCTACTACGGACGTGATCGCGTCCTTCCTCAGAGCTCTAGAGG
AGGAGAACGAGGACGAACTCCGACCGTACGAAGTCTTGTCCGGGGATTTCCACGGGCTCACGCCGGAACTGTCCGCTCCG
GTGACCGGTCCGACGGTACCGGTCGAGGACGTACTCCTGACGTCGAACAAGGACCTGTGCCTGGGCTGCGACCTGGACGT
GATTGAGAAGGACCTCTTCTGCGTCGGCTGCGGAACGTGTGCCGCCTCCTGCCCGGCGTGGGCTATCGAGATGGACGAGA
AGCCCGTAATCCGGCAAGAGAGGTGCGTTCGATGCGGAACTTGCTTCGTAAGCTGCCCGAGATCGTTCAGGATATGA

Upstream 100 bases:

>100_bases
GGGGAGATGGTGCGCGACCTGACGGTTGACAGGGCTGAGACAGTTCTACGCGCGATCGGCCTCTCCGGTAGATGTCTTAC
CCACTAGGGGGAAGCCCAAG

Downstream 100 bases:

>100_bases
ACCCAACGGGAGGTGATCCGTGTTGCGCAAGCTGATCCGAAGAATAGCAGGGGGCAAAGAGGAAGAGAATACCCGTGAGG
GATCTCCCGAGAACGATCTC

Product: coenzyme F420-reducing hydrogenase, gamma subunit

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKVAVLSLMACGTCARNLEEVCHEEGHEVVWSNLEGDEGEPDADILFVEGAVDFADAETLEKLEEAASAVSTVVSLGSCA
ATGGFERHVIGMRDPDRYHFTVFPAAKFVETDYAINGCPPTTDVIASFLRALEEENEDELRPYEVLSGDFHGLTPELSAP
VTGPTVPVEDVLLTSNKDLCLGCDLDVIEKDLFCVGCGTCAASCPAWAIEMDEKPVIRQERCVRCGTCFVSCPRSFRI

Sequences:

>Translated_238_residues
MKVAVLSLMACGTCARNLEEVCHEEGHEVVWSNLEGDEGEPDADILFVEGAVDFADAETLEKLEEAASAVSTVVSLGSCA
ATGGFERHVIGMRDPDRYHFTVFPAAKFVETDYAINGCPPTTDVIASFLRALEEENEDELRPYEVLSGDFHGLTPELSAP
VTGPTVPVEDVLLTSNKDLCLGCDLDVIEKDLFCVGCGTCAASCPAWAIEMDEKPVIRQERCVRCGTCFVSCPRSFRI
>Mature_238_residues
MKVAVLSLMACGTCARNLEEVCHEEGHEVVWSNLEGDEGEPDADILFVEGAVDFADAETLEKLEEAASAVSTVVSLGSCA
ATGGFERHVIGMRDPDRYHFTVFPAAKFVETDYAINGCPPTTDVIASFLRALEEENEDELRPYEVLSGDFHGLTPELSAP
VTGPTVPVEDVLLTSNKDLCLGCDLDVIEKDLFCVGCGTCAASCPAWAIEMDEKPVIRQERCVRCGTCFVSCPRSFRI

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1941

COG function: function code C; Coenzyme F420-reducing hydrogenase, gamma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006137
- InterPro:   IPR014406 [H]

Pfam domain/function: PF00037 Fer4; PF01058 Oxidored_q6 [H]

EC number: 1.12.98.1

Molecular weight: Translated: 25776; Mature: 25776

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
6.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLSLMACGTCARNLEEVCHEEGHEVVWSNLEGDEGEPDADILFVEGAVDFADAETL
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHH
EKLEEAASAVSTVVSLGSCAATGGFERHVIGMRDPDRYHFTVFPAAKFVETDYAINGCPP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCHHHHHCCCEECCCCC
TTDVIASFLRALEEENEDELRPYEVLSGDFHGLTPELSAPVTGPTVPVEDVLLTSNKDLC
CHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCEE
LGCDLDVIEKDLFCVGCGTCAASCPAWAIEMDEKPVIRQERCVRCGTCFVSCPRSFRI
EECCHHHHCCCEEEEECCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MKVAVLSLMACGTCARNLEEVCHEEGHEVVWSNLEGDEGEPDADILFVEGAVDFADAETL
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHH
EKLEEAASAVSTVVSLGSCAATGGFERHVIGMRDPDRYHFTVFPAAKFVETDYAINGCPP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCHHHHHCCCEECCCCC
TTDVIASFLRALEEENEDELRPYEVLSGDFHGLTPELSAPVTGPTVPVEDVLLTSNKDLC
CHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCEE
LGCDLDVIEKDLFCVGCGTCAASCPAWAIEMDEKPVIRQERCVRCGTCFVSCPRSFRI
EECCHHHHCCCEEEEECCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]