Click here to switch to the map view.

The map label for this gene is map

Identifier: 18311365

GI number: 18311365

Start: 2740586

End: 2741335

Strand: Reverse

Name: map

Synonym: CPE2383

Alternate gene names: 18311365

Gene position: 2741335-2740586 (Counterclockwise)

Preceding gene: 18311366

Following gene: 18311364

Centisome position: 90.43

GC content: 35.87

Gene sequence:

>750_bases
ATGATAATTCTTAAGAATGAGAATGAAATCGACCTAATGAGGAAAGCAGGAAAGATTCTTGGAGAGACACTTAACCTGCT
TAAGGAGAAAGTAAGACCTGGTATTACTACTACGGAATTAGATAGAATAGCTGAAGAGTTTATAACTAAGCATGGAGCTA
CACCATCCTTCAAAGGATTGTATGGTTTCCCAGCTTCATTATGTATATCTGTTAATAATCAAGTAATTCATGGTTTCCCA
GGAAGTTATGAATTAAAGGATGGAGACATTGTTTCTATTGATTGTGGAGTTTGTCTAAATGGATTTCACAGTGATGCTGC
TAGAACTTTTGGTGTAGGCAATATATCAGAAGAGGCAGAAAAGCTTATTAGAATTACTGAAGAATCCTTTTTTAAAGGTA
TAGAAAAGGCATATGTAGACAATAGATTAACGGATATTTCTAATGAAATACAGCAATATGTTGAAGCCAATGGATTTTCT
GTTGTTAGAGACTTTGTAGGACATGGAATTGGCCGAAAAGTCCATGAAGATCCTGAAGTTCCTAATTTCGGTAGACCAGG
CAGAGGTCCAAAGTTAATGGCAGGAATGGTATTAGCTATTGAACCGATGGTAAACATGGGATCATATAGAGTCAAGACTT
TAGATGATGGCTGGACAGTAGTAACAGCAGATGGGAAACTTTCTGCACACTATGAAAACACAGTTGCAATTTTACCTAAT
GGTCCTGAAATATTAACACTTATAAAATAG

Upstream 100 bases:

>100_bases
ATTTTTATAAAAACAAAAAGCAACTTTCAGTAGTAGATGGAACACAAGCAATAAACGAGGTCTTTGAGAGTATCTGTAAA
ATATTAGGGAGCGACAAGTA

Downstream 100 bases:

>100_bases
TTTTAATATATATTGCTTAGTTATAAATTAACATTTGCCTATAAGGAATTTGTAGTTTATGACTAAGGTAATCTTTGAGG
TGGATAAGTTGCAAGAAACT

Product: methionine aminopeptidase, type I

Products: NA

Alternate protein names: MAP; Peptidase M

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIILKNENEIDLMRKAGKILGETLNLLKEKVRPGITTTELDRIAEEFITKHGATPSFKGLYGFPASLCISVNNQVIHGFP
GSYELKDGDIVSIDCGVCLNGFHSDAARTFGVGNISEEAEKLIRITEESFFKGIEKAYVDNRLTDISNEIQQYVEANGFS
VVRDFVGHGIGRKVHEDPEVPNFGRPGRGPKLMAGMVLAIEPMVNMGSYRVKTLDDGWTVVTADGKLSAHYENTVAILPN
GPEILTLIK

Sequences:

>Translated_249_residues
MIILKNENEIDLMRKAGKILGETLNLLKEKVRPGITTTELDRIAEEFITKHGATPSFKGLYGFPASLCISVNNQVIHGFP
GSYELKDGDIVSIDCGVCLNGFHSDAARTFGVGNISEEAEKLIRITEESFFKGIEKAYVDNRLTDISNEIQQYVEANGFS
VVRDFVGHGIGRKVHEDPEVPNFGRPGRGPKLMAGMVLAIEPMVNMGSYRVKTLDDGWTVVTADGKLSAHYENTVAILPN
GPEILTLIK
>Mature_249_residues
MIILKNENEIDLMRKAGKILGETLNLLKEKVRPGITTTELDRIAEEFITKHGATPSFKGLYGFPASLCISVNNQVIHGFP
GSYELKDGDIVSIDCGVCLNGFHSDAARTFGVGNISEEAEKLIRITEESFFKGIEKAYVDNRLTDISNEIQQYVEANGFS
VVRDFVGHGIGRKVHEDPEVPNFGRPGRGPKLMAGMVLAIEPMVNMGSYRVKTLDDGWTVVTADGKLSAHYENTVAILPN
GPEILTLIK

Specific function: Removes the amino-terminal methionine from nascent proteins

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family

Homologues:

Organism=Homo sapiens, GI164420681, Length=246, Percent_Identity=37.3983739837398, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI40385867, Length=245, Percent_Identity=38.7755102040816, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1786364, Length=250, Percent_Identity=44.4, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI1789275, Length=224, Percent_Identity=28.125, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996291, Length=247, Percent_Identity=38.0566801619433, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323273, Length=247, Percent_Identity=39.6761133603239, Blast_Score=180, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21355531, Length=245, Percent_Identity=42.0408163265306, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24583427, Length=245, Percent_Identity=35.9183673469388, Blast_Score=164, Evalue=5e-41,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): AMPM_CLOPE (P50614)

Other databases:

- EMBL:   BA000016
- EMBL:   X86486
- RefSeq:   NP_563299.1
- ProteinModelPortal:   P50614
- SMR:   P50614
- MEROPS:   M24.001
- GeneID:   990744
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2383
- NMPDR:   fig|195102.1.peg.2446
- HOGENOM:   HBG299384
- OMA:   EPMVCQK
- PhylomeDB:   P50614
- ProtClustDB:   CLSK811239
- BioCyc:   CPER195102:CPE2383-MONOMER
- BRENDA:   3.4.11.18
- GO:   GO:0006508
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804:SF13
- PANTHER:   PTHR10804
- PRINTS:   PR00599
- TIGRFAMs:   TIGR00500

Pfam domain/function: PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: =3.4.11.18

Molecular weight: Translated: 27324; Mature: 27324

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00680 MAP_1

Important sites: BINDING 77-77 BINDING 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILKNENEIDLMRKAGKILGETLNLLKEKVRPGITTTELDRIAEEFITKHGATPSFKGL
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
YGFPASLCISVNNQVIHGFPGSYELKDGDIVSIDCGVCLNGFHSDAARTFGVGNISEEAE
CCCCCEEEEEECCEEEECCCCCEEECCCCEEEEECCCEECCCCCHHHHHCCCCCHHHHHH
KLIRITEESFFKGIEKAYVDNRLTDISNEIQQYVEANGFSVVRDFVGHGIGRKVHEDPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
PNFGRPGRGPKLMAGMVLAIEPMVNMGSYRVKTLDDGWTVVTADGKLSAHYENTVAILPN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCEEEEEECCEEEECCC
GPEILTLIK
CCCEEEEEC
>Mature Secondary Structure
MIILKNENEIDLMRKAGKILGETLNLLKEKVRPGITTTELDRIAEEFITKHGATPSFKGL
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
YGFPASLCISVNNQVIHGFPGSYELKDGDIVSIDCGVCLNGFHSDAARTFGVGNISEEAE
CCCCCEEEEEECCEEEECCCCCEEECCCCEEEEECCCEECCCCCHHHHHCCCCCHHHHHH
KLIRITEESFFKGIEKAYVDNRLTDISNEIQQYVEANGFSVVRDFVGHGIGRKVHEDPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
PNFGRPGRGPKLMAGMVLAIEPMVNMGSYRVKTLDDGWTVVTADGKLSAHYENTVAILPN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCEEEEEECCEEEECCC
GPEILTLIK
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358