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The map label for this gene is cwlD [H]

Identifier: 18311349

GI number: 18311349

Start: 2729132

End: 2729803

Strand: Reverse

Name: cwlD [H]

Synonym: CPE2367

Alternate gene names: 18311349

Gene position: 2729803-2729132 (Counterclockwise)

Preceding gene: 18311350

Following gene: 18311346

Centisome position: 90.05

GC content: 30.21

Gene sequence:

>672_bases
ATGAAGAAAATAATGAAAATAGTGGCAATTATGATGTTCTCTTTTTTAGTATTAAATATTAGCTTATTAAAGGTTAATGC
AGAGGAAAATAATAAGGTAATAGTTATTGACCCAGGTCATGGAGGCATAGATGGTGGCGCAAAGAGTGAAAATGGAGTAA
TTGAGAAGGACATTAATTTATCAATATCACTTAAAACAAAAGCTGCCTTAGAGAGTAAGGGATATAAAGTCATAATGACT
AGAAGTGAGGATGTAGGACTATATACTGAGGGCAAAAAGGTTAGAGAAAAGAAAATAGAAGACTTAGGAAATAGAGTTAA
GATTAAAAAAGAAAATAAGTGTGATGCTTTTATAAGCATACATCAAAATATGTTTCCTCAAAAGAATTGTAAAGGGGCAC
AAGTATGGAGTGCCAACAATGAACCAAGTCAAAAGCTAGGCAAAATAATACAACAAAAATTTAAGGATGAAGTTGACCCA
AATAATAAGAGGGAAGCAAAAGTTGCAAAAAAAGAATATAAGATTTTAAATGATGGATATGAAGGAGCTTCTGTAATAGT
TGAATGTGGTTTCTTATCTAATCCAGAAGAATGCGAACTATTAGGTAAAGAAGATTATCAAAATAAAATTGCAAATACCT
TAGCAAATGCTATAGATGAATATTTTAAATAA

Upstream 100 bases:

>100_bases
AATTCTAAAAGAGATAATATTTTGAAGTATTTACTAGTAAATATATTCTTTTTAGGATTTAGGATTATAATAATTTAGAT
TTAAATAGGAGGTATTCTTA

Downstream 100 bases:

>100_bases
TATAATTTAAAGAAAATTTAGTAATGGCAAACTAACTTCCATATCATTTGGGGTAATTGAGTAAAATTTATAAGATGAAA
ATATAAGGTTTATAATTAAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKKIMKIVAIMMFSFLVLNISLLKVNAEENNKVIVIDPGHGGIDGGAKSENGVIEKDINLSISLKTKAALESKGYKVIMT
RSEDVGLYTEGKKVREKKIEDLGNRVKIKKENKCDAFISIHQNMFPQKNCKGAQVWSANNEPSQKLGKIIQQKFKDEVDP
NNKREAKVAKKEYKILNDGYEGASVIVECGFLSNPEECELLGKEDYQNKIANTLANAIDEYFK

Sequences:

>Translated_223_residues
MKKIMKIVAIMMFSFLVLNISLLKVNAEENNKVIVIDPGHGGIDGGAKSENGVIEKDINLSISLKTKAALESKGYKVIMT
RSEDVGLYTEGKKVREKKIEDLGNRVKIKKENKCDAFISIHQNMFPQKNCKGAQVWSANNEPSQKLGKIIQQKFKDEVDP
NNKREAKVAKKEYKILNDGYEGASVIVECGFLSNPEECELLGKEDYQNKIANTLANAIDEYFK
>Mature_223_residues
MKKIMKIVAIMMFSFLVLNISLLKVNAEENNKVIVIDPGHGGIDGGAKSENGVIEKDINLSISLKTKAALESKGYKVIMT
RSEDVGLYTEGKKVREKKIEDLGNRVKIKKENKCDAFISIHQNMFPQKNCKGAQVWSANNEPSQKLGKIIQQKFKDEVDP
NNKREAKVAKKEYKILNDGYEGASVIVECGFLSNPEECELLGKEDYQNKIANTLANAIDEYFK

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=233, Percent_Identity=28.755364806867, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082163, Length=106, Percent_Identity=33.9622641509434, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24988; Mature: 24988

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIMKIVAIMMFSFLVLNISLLKVNAEENNKVIVIDPGHGGIDGGAKSENGVIEKDINL
CHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCE
SISLKTKAALESKGYKVIMTRSEDVGLYTEGKKVREKKIEDLGNRVKIKKENKCDAFISI
EEEEEHHHHHHCCCEEEEEECCCCCCEEECCHHHHHHHHHHCCCEEEEEECCCCHHHHHH
HQNMFPQKNCKGAQVWSANNEPSQKLGKIIQQKFKDEVDPNNKREAKVAKKEYKILNDGY
HHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
EGASVIVECGFLSNPEECELLGKEDYQNKIANTLANAIDEYFK
CCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIMKIVAIMMFSFLVLNISLLKVNAEENNKVIVIDPGHGGIDGGAKSENGVIEKDINL
CHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCE
SISLKTKAALESKGYKVIMTRSEDVGLYTEGKKVREKKIEDLGNRVKIKKENKCDAFISI
EEEEEHHHHHHCCCEEEEEECCCCCCEEECCHHHHHHHHHHCCCEEEEEECCCCHHHHHH
HQNMFPQKNCKGAQVWSANNEPSQKLGKIIQQKFKDEVDPNNKREAKVAKKEYKILNDGY
HHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
EGASVIVECGFLSNPEECELLGKEDYQNKIANTLANAIDEYFK
CCCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]