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The map label for this gene is mleN [H]

Identifier: 18311299

GI number: 18311299

Start: 2663655

End: 2664968

Strand: Reverse

Name: mleN [H]

Synonym: CPE2317

Alternate gene names: 18311299

Gene position: 2664968-2663655 (Counterclockwise)

Preceding gene: 18311300

Following gene: 18311297

Centisome position: 87.91

GC content: 30.52

Gene sequence:

>1314_bases
ATGAAAAAAGGAAAGTTTTCAGCATTATTACCATTAATAATTTTTGTATCGATTTATTTGGGAACTTCATTAGTAATGAA
AGATTTCTACTCTGTATCTGTTTTAGTTCCAGGAATAATTGCGGTTTTATTTGGAATATTTATAAATAGAAAAAGAGGAC
TTGAAAAGAACATAGAGATATTTTGTAAGGGGGCTGGTAATAGTAATATAATTTTAATGTGCTTAATCTTTATATTAGCT
GGTGCCTTTGCAGAGGTTGCTAAAAATATGGGAGCTGTAGAATCTACAGTAAATTTAGGGCTAACAATATTACCTAAAAA
TATATTAGTAGCAGGAATATTTATAATATCATCATTTATAGCTATATCTTTAGGAACATCAATGGGAACAATAGCTGCTA
TAGTTCCAATAGCAATGGGAATTTCAGATAAGACTAATATAGCATTACCACTTATAGTTGGGGCAGTAGTAGGTGGAGCT
ATGTTTGGGGACAATCTATCAATGATTTCTGATACAACAATAGCAGCAACAAAAACTCAAGGATGTGAAATGAAGGATAA
GTTTAAAATAAACTTTAAGGTTATATTACCAGCTGCTATCTTAGTAATTGTAATATATACATTCTTAGGAAGTGGGGCTA
ATACATCAGTAGGTCAATATGATTATAATTTTATTAAGATTATTCCTTACTTAGGTATTTTAATAGCTGCATTAATGGGG
GCTAATGTAATTGCTATATTATCAGGTGGAATAGTATTAGCTGGAGCAATTGGATTCTTCACAGGATCTTTAGATTTAAA
GACTTTCTTTACTTCAATATCAACTGGTATAGGGGGAATGAGTGAACTTATATTAATTTCAATAATAATTGGGGGAATTG
TTGAAATAATAAAAGAAAATGGTGGTATAGACTATGTATTAAACTTAGTTACTAAGAGAATCAATGGCAAAAAAGGGGCT
GAATTTGGTATTGGATTATTAGTAAGCTTAGTTGATGCATGTACTGCAAATAATACTATTGCTATAGTTACAGTTGGACC
TTTAGCAAAGGATATTTCAGATGAATATAACTTAGATTCAAAAAGAATTGCAGGAATATTAGATATGTTCTCTTGTGCAG
TTCAGGGAATAATTCCATATGGAGCACAATTAATGTCAGCGGCGGCTTTAACTGGATTAAGTTCATTTGAAATAATGAAA
TTTTTATTCTATCCATATTTAATGGGAATAAGTGCAATAGCATTCATAGCATTTTTCCAAAAGAAAGAAAGTGCTGAAAA
AAGTGGGGAGTTAAATGCTATAGCAGTTGAATAA

Upstream 100 bases:

>100_bases
AAGAGAGTCTGTAAGAATAATAACTTCTATCCTAGGATAGGAGTTTTTTTATTTTGTAGGATAAAGGATAGATTTATTAA
ATGGATTAGGAGGAGAGAAA

Downstream 100 bases:

>100_bases
AATATTAAAAAGCAATAAGCGTTAAAAAGGGGATTTATATAAAAGGGGTTTAATAAATTTTAGTATAAAGTAAAAAAAGA
TAGGCTATAGAAAATAAAAC

Product: Na+/H+ antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKKGKFSALLPLIIFVSIYLGTSLVMKDFYSVSVLVPGIIAVLFGIFINRKRGLEKNIEIFCKGAGNSNIILMCLIFILA
GAFAEVAKNMGAVESTVNLGLTILPKNILVAGIFIISSFIAISLGTSMGTIAAIVPIAMGISDKTNIALPLIVGAVVGGA
MFGDNLSMISDTTIAATKTQGCEMKDKFKINFKVILPAAILVIVIYTFLGSGANTSVGQYDYNFIKIIPYLGILIAALMG
ANVIAILSGGIVLAGAIGFFTGSLDLKTFFTSISTGIGGMSELILISIIIGGIVEIIKENGGIDYVLNLVTKRINGKKGA
EFGIGLLVSLVDACTANNTIAIVTVGPLAKDISDEYNLDSKRIAGILDMFSCAVQGIIPYGAQLMSAAALTGLSSFEIMK
FLFYPYLMGISAIAFIAFFQKKESAEKSGELNAIAVE

Sequences:

>Translated_437_residues
MKKGKFSALLPLIIFVSIYLGTSLVMKDFYSVSVLVPGIIAVLFGIFINRKRGLEKNIEIFCKGAGNSNIILMCLIFILA
GAFAEVAKNMGAVESTVNLGLTILPKNILVAGIFIISSFIAISLGTSMGTIAAIVPIAMGISDKTNIALPLIVGAVVGGA
MFGDNLSMISDTTIAATKTQGCEMKDKFKINFKVILPAAILVIVIYTFLGSGANTSVGQYDYNFIKIIPYLGILIAALMG
ANVIAILSGGIVLAGAIGFFTGSLDLKTFFTSISTGIGGMSELILISIIIGGIVEIIKENGGIDYVLNLVTKRINGKKGA
EFGIGLLVSLVDACTANNTIAIVTVGPLAKDISDEYNLDSKRIAGILDMFSCAVQGIIPYGAQLMSAAALTGLSSFEIMK
FLFYPYLMGISAIAFIAFFQKKESAEKSGELNAIAVE
>Mature_437_residues
MKKGKFSALLPLIIFVSIYLGTSLVMKDFYSVSVLVPGIIAVLFGIFINRKRGLEKNIEIFCKGAGNSNIILMCLIFILA
GAFAEVAKNMGAVESTVNLGLTILPKNILVAGIFIISSFIAISLGTSMGTIAAIVPIAMGISDKTNIALPLIVGAVVGGA
MFGDNLSMISDTTIAATKTQGCEMKDKFKINFKVILPAAILVIVIYTFLGSGANTSVGQYDYNFIKIIPYLGILIAALMG
ANVIAILSGGIVLAGAIGFFTGSLDLKTFFTSISTGIGGMSELILISIIIGGIVEIIKENGGIDYVLNLVTKRINGKKGA
EFGIGLLVSLVDACTANNTIAIVTVGPLAKDISDEYNLDSKRIAGILDMFSCAVQGIIPYGAQLMSAAALTGLSSFEIMK
FLFYPYLMGISAIAFIAFFQKKESAEKSGELNAIAVE

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 45951; Mature: 45951

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGKFSALLPLIIFVSIYLGTSLVMKDFYSVSVLVPGIIAVLFGIFINRKRGLEKNIEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
FCKGAGNSNIILMCLIFILAGAFAEVAKNMGAVESTVNLGLTILPKNILVAGIFIISSFI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEECHHHHHHHHHHHHHHH
AISLGTSMGTIAAIVPIAMGISDKTNIALPLIVGAVVGGAMFGDNLSMISDTTIAATKTQ
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCEEEEECCC
GCEMKDKFKINFKVILPAAILVIVIYTFLGSGANTSVGQYDYNFIKIIPYLGILIAALMG
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ANVIAILSGGIVLAGAIGFFTGSLDLKTFFTSISTGIGGMSELILISIIIGGIVEIIKEN
CCEEEHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
GGIDYVLNLVTKRINGKKGAEFGIGLLVSLVDACTANNTIAIVTVGPLAKDISDEYNLDS
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCHHCCCCH
KRIAGILDMFSCAVQGIIPYGAQLMSAAALTGLSSFEIMKFLFYPYLMGISAIAFIAFFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKESAEKSGELNAIAVE
HHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MKKGKFSALLPLIIFVSIYLGTSLVMKDFYSVSVLVPGIIAVLFGIFINRKRGLEKNIEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
FCKGAGNSNIILMCLIFILAGAFAEVAKNMGAVESTVNLGLTILPKNILVAGIFIISSFI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEECHHHHHHHHHHHHHHH
AISLGTSMGTIAAIVPIAMGISDKTNIALPLIVGAVVGGAMFGDNLSMISDTTIAATKTQ
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCEEEEECCC
GCEMKDKFKINFKVILPAAILVIVIYTFLGSGANTSVGQYDYNFIKIIPYLGILIAALMG
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ANVIAILSGGIVLAGAIGFFTGSLDLKTFFTSISTGIGGMSELILISIIIGGIVEIIKEN
CCEEEHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
GGIDYVLNLVTKRINGKKGAEFGIGLLVSLVDACTANNTIAIVTVGPLAKDISDEYNLDS
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCHHCCCCH
KRIAGILDMFSCAVQGIIPYGAQLMSAAALTGLSSFEIMKFLFYPYLMGISAIAFIAFFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKESAEKSGELNAIAVE
HHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]