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The map label for this gene is rluF [H]

Identifier: 18311295

GI number: 18311295

Start: 2659981

End: 2660886

Strand: Reverse

Name: rluF [H]

Synonym: CPE2313

Alternate gene names: 18311295

Gene position: 2660886-2659981 (Counterclockwise)

Preceding gene: 18311296

Following gene: 18311293

Centisome position: 87.78

GC content: 27.92

Gene sequence:

>906_bases
GTGAGAAAAGCATCAAAAAATAACAATAGAAGAGACAATAGTTTATCTAATAAAAAGTTTAAGAGTGAAGATAAAAATTT
AAATAAAACTAATAAAAATAATAAAAATAATAAACAAAAAGTAGCTAAAAAAGCTTCAGCAAAAAATACTATTATAGTAT
CAAATAAAAATGAGATAAGAGATGAAGTTAGATTAAATAAATATATAAGTGAAACAGGATTTTGCTCAAGGAGAGAGGCT
GATAAGCTTATAGAACAAGGAAGAGTTAAGATAGATGGGGTAAAGGCTACAACTGGTATGAAGGTTTCTAATGGGCAAAG
TGTATCTATTGATGGAAAATCTCTAAAGGTTGAAAATGAACTAGTTTATATAGCTTTAAATAAGCCTGTTGGAATAACTT
GTACTACTGAGAGTAAGATAAAAGGCAATATAGTTGACTTTATAAATCATGAAAAAAGAATTTTCCCAATAGGAAGACTA
GATAAGGATTCTCAAGGATTAATATTTATGACTAATGATGGTGATATAGTTAATAAGATATTAAGAGCTGGTAATAATCA
TGAAAAAGAATATATTGTCACTGTAAATAAGCCTATAACTGATGAGTTTATAAAAGGAATGAGCAATGGAGTGCCTATAC
TAGGAACAGTTACTAAGAAATGCTTAGTTAAGAAAGAAAGCAAAAACTCATTTAGAATAATATTAACTCAAGGGTTAAAT
AGACAGATAAGAAGAATGTGTGAGTATTTTGGATATGAGGTAAAAAAATTAGAAAGAATAAGAATAATGAATGTTAGCCT
AGGAAACCTAAAAATGGGATCTTGGAGATACTTAACTAAGAAAGAATTAGCTGAAATAAACAGGTTAACAGAAAACTCTT
CAAAGACTGAAGAGGCCTCAATTTAA

Upstream 100 bases:

>100_bases
GAGTGTAAATAAGGATGATAATGCAAAGAATAATTTTAGAATAAAAATAAATAATTCAGGCTATGAGAAGAAAAATTAAT
TAAGGAAAGGATTATTTATA

Downstream 100 bases:

>100_bases
TTGAATGTTAATTACTAGATATATATTCAGGCCTAACTTAATTTCGCAAAGAGTTTCTATTGTTTATTCCAGAATAATAG
CTAAGGCTTTTAAACTCGCT

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRKASKNNNRRDNSLSNKKFKSEDKNLNKTNKNNKNNKQKVAKKASAKNTIIVSNKNEIRDEVRLNKYISETGFCSRREA
DKLIEQGRVKIDGVKATTGMKVSNGQSVSIDGKSLKVENELVYIALNKPVGITCTTESKIKGNIVDFINHEKRIFPIGRL
DKDSQGLIFMTNDGDIVNKILRAGNNHEKEYIVTVNKPITDEFIKGMSNGVPILGTVTKKCLVKKESKNSFRIILTQGLN
RQIRRMCEYFGYEVKKLERIRIMNVSLGNLKMGSWRYLTKKELAEINRLTENSSKTEEASI

Sequences:

>Translated_301_residues
MRKASKNNNRRDNSLSNKKFKSEDKNLNKTNKNNKNNKQKVAKKASAKNTIIVSNKNEIRDEVRLNKYISETGFCSRREA
DKLIEQGRVKIDGVKATTGMKVSNGQSVSIDGKSLKVENELVYIALNKPVGITCTTESKIKGNIVDFINHEKRIFPIGRL
DKDSQGLIFMTNDGDIVNKILRAGNNHEKEYIVTVNKPITDEFIKGMSNGVPILGTVTKKCLVKKESKNSFRIILTQGLN
RQIRRMCEYFGYEVKKLERIRIMNVSLGNLKMGSWRYLTKKELAEINRLTENSSKTEEASI
>Mature_301_residues
MRKASKNNNRRDNSLSNKKFKSEDKNLNKTNKNNKNNKQKVAKKASAKNTIIVSNKNEIRDEVRLNKYISETGFCSRREA
DKLIEQGRVKIDGVKATTGMKVSNGQSVSIDGKSLKVENELVYIALNKPVGITCTTESKIKGNIVDFINHEKRIFPIGRL
DKDSQGLIFMTNDGDIVNKILRAGNNHEKEYIVTVNKPITDEFIKGMSNGVPILGTVTKKCLVKKESKNSFRIILTQGLN
RQIRRMCEYFGYEVKKLERIRIMNVSLGNLKMGSWRYLTKKELAEINRLTENSSKTEEASI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=68.3760683760684, Blast_Score=310, Evalue=7e-86,
Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=29.5652173913043, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=31.4741035856574, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 34206; Mature: 34206

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKASKNNNRRDNSLSNKKFKSEDKNLNKTNKNNKNNKQKVAKKASAKNTIIVSNKNEIR
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHH
DEVRLNKYISETGFCSRREADKLIEQGRVKIDGVKATTGMKVSNGQSVSIDGKSLKVENE
HHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCEEECCCCEEEECCCEEEEECE
LVYIALNKPVGITCTTESKIKGNIVDFINHEKRIFPIGRLDKDSQGLIFMTNDGDIVNKI
EEEEEECCCEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHH
LRAGNNHEKEYIVTVNKPITDEFIKGMSNGVPILGTVTKKCLVKKESKNSFRIILTQGLN
HHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCCC
RQIRRMCEYFGYEVKKLERIRIMNVSLGNLKMGSWRYLTKKELAEINRLTENSSKTEEAS
HHHHHHHHHHCCHHHHHCEEEEEEEEECCEECCCEEEHHHHHHHHHHHHHCCCCCCCCCC
I
C
>Mature Secondary Structure
MRKASKNNNRRDNSLSNKKFKSEDKNLNKTNKNNKNNKQKVAKKASAKNTIIVSNKNEIR
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHH
DEVRLNKYISETGFCSRREADKLIEQGRVKIDGVKATTGMKVSNGQSVSIDGKSLKVENE
HHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCEEECCCCEEEECCCEEEEECE
LVYIALNKPVGITCTTESKIKGNIVDFINHEKRIFPIGRLDKDSQGLIFMTNDGDIVNKI
EEEEEECCCEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHH
LRAGNNHEKEYIVTVNKPITDEFIKGMSNGVPILGTVTKKCLVKKESKNSFRIILTQGLN
HHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCCC
RQIRRMCEYFGYEVKKLERIRIMNVSLGNLKMGSWRYLTKKELAEINRLTENSSKTEEAS
HHHHHHHHHHCCHHHHHCEEEEEEEEECCEECCCEEEHHHHHHHHHHHHHCCCCCCCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]