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The map label for this gene is appA [H]

Identifier: 18311255

GI number: 18311255

Start: 2611027

End: 2612766

Strand: Reverse

Name: appA [H]

Synonym: CPE2273

Alternate gene names: 18311255

Gene position: 2612766-2611027 (Counterclockwise)

Preceding gene: 18311257

Following gene: 18311254

Centisome position: 86.19

GC content: 32.53

Gene sequence:

>1740_bases
ATGAAAAAAAGAAAATTAGTTGCATTATTAACGGCGGGGTTAGCAGCTTCAATGTTATTTGTAGCATGTGGAGGAGCTAA
CAATACAGCTCAAGGAAATGGTAATGGTTCAGAATCAGGAGGAACTACTAAGGATTTATCAAAGCCAGAGAGAATAGAGG
CATCAAATCCTAGTGCACTTCCAGATGCTGCAAAAAATAGAACTGATACTTTAATAGTAGGAACTACAGATCCAAAGGGT
GAATTTGTTCCAATATATTCTTCTACTCTTTATGATTCATGGGTTAACAAGTTAGTATTTGATGGATTGATTACTAATAA
TGAAAAGGGAGAACCAATTCCAAATGTAGCAGAAAGTTATGAAGTTTCTGAAGATGGAAAAACTTATACATTTAAATTAA
ATAAGGGTATTAAATTTACTAATGGTCAAGAATTAACAGCAAAGGATGTAGCATTTACATTTACTTCTATTTGTGATCCA
GGATATGATGGACCAAGAATGGATGCTGTAAATAATTTAGTTGGATATGAAGAGTACAATAAGGGCGATGCTAGTAGTGT
TGAAGGTATAAAGGTTATTGATGATTATACAATATCATTCACTAACAAGAATACTGATGCAGCTGGTATATGGAATTTTG
AATATGGAATTATGCCTGAAAGTGTTTATAAATTTGAAAAAGGAAACTTCCAAGCTGTTAAGGATAAATTATTAGAGCCA
GTAGGTTCAGGTGCTTATAAATTTGTTCACTTTAAACCAGGACAAGAAGTTAAGTTTGAAAAAAATGCTGATTACTGGAA
AGGGGAGCCGAAGATTCCTTATATAGTAATGAAAGTTACAAATGCACAAACATTATTACAAGAATTAATGGCTGGAACAG
TTGATATAGATAGAGTTGGTGCTAAACCAGAAAATATAGATCCATTAAAACAAGCTGGATTCTTAAACTTAGATCTATAT
ATGCAAAATGGTTATGGATACATGGGACTTAACTATGGAAGTGATAAGGTTAAAGACCCTAAAGTAAGACAAGCGTTACT
TTATGGATTAAATAGAGAAGGATTCATGCAATCTTATTACCAAGGATATGGTCAAGTTTACAACTCACACATTCTTCCTA
CTTCATGGGCATATAACCCAGATGTTCCTAAGTATGAATATAATCCAGAAAAAGCTAAAGAATTACTTGATGAAGCTGGT
TGGAAAGATACAAATGGAAATGGAGTTAGAGATAAGGATGGAGTTGAATTAGAACTTCAATGGTTAACTTATACTGGTTC
TAAATATGTTGATGCTTTAATCCCAATAGTTCAACAATCTTGGGAACAAATAGGTGTTAAAGTTACTCCAGAACTTATGG
AATTTGGAACAATGATGGATAAAGTTAATAACAGAGAATATGATATATTCAATGGTGCTTGGAACCTTTCAATAGATCCA
GATCCATCAGGAATATTTGCAATCTCTCAAGATGTACCAGGCGGATTTAATAATATTGGATGGAGAAATGAAGAAGCAGA
TAAGTTATTAAAAGAAGGTAAAGGAACAACAAATCAAGAGGAAAGAAAGAAAGCTTATGCTGAATGGCAATTAAAATTCT
CTGAAGATGTACCTTATATTCTTCTTGGAAATGCTCAAGAAATGTTTGCATCAAATTCAAGAGTTAAAGGATACAACCCT
TCAACTTATATAGATTGGACTCACGATGTTTATAAACTTGAATTAGATAACAATAAATAA

Upstream 100 bases:

>100_bases
AATCACATTAAATGTATGTTATTGTAACAATATTTTTTATATGGGGCATTTAAATTTTGTTACATAACAGCAATAAATAT
ATTAAAAGGGGGCAGATTAA

Downstream 100 bases:

>100_bases
AATTTAAAATAAATTAAGAAATTAGCCTGGACAAAAAATCCAGGCTAATTTTGAAAAATGGGGGGAACAGCAATATGCTA
CAGTATATCACTAGAAGAAT

Product: oligopeptide ABC transporter

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MKKRKLVALLTAGLAASMLFVACGGANNTAQGNGNGSESGGTTKDLSKPERIEASNPSALPDAAKNRTDTLIVGTTDPKG
EFVPIYSSTLYDSWVNKLVFDGLITNNEKGEPIPNVAESYEVSEDGKTYTFKLNKGIKFTNGQELTAKDVAFTFTSICDP
GYDGPRMDAVNNLVGYEEYNKGDASSVEGIKVIDDYTISFTNKNTDAAGIWNFEYGIMPESVYKFEKGNFQAVKDKLLEP
VGSGAYKFVHFKPGQEVKFEKNADYWKGEPKIPYIVMKVTNAQTLLQELMAGTVDIDRVGAKPENIDPLKQAGFLNLDLY
MQNGYGYMGLNYGSDKVKDPKVRQALLYGLNREGFMQSYYQGYGQVYNSHILPTSWAYNPDVPKYEYNPEKAKELLDEAG
WKDTNGNGVRDKDGVELELQWLTYTGSKYVDALIPIVQQSWEQIGVKVTPELMEFGTMMDKVNNREYDIFNGAWNLSIDP
DPSGIFAISQDVPGGFNNIGWRNEEADKLLKEGKGTTNQEERKKAYAEWQLKFSEDVPYILLGNAQEMFASNSRVKGYNP
STYIDWTHDVYKLELDNNK

Sequences:

>Translated_579_residues
MKKRKLVALLTAGLAASMLFVACGGANNTAQGNGNGSESGGTTKDLSKPERIEASNPSALPDAAKNRTDTLIVGTTDPKG
EFVPIYSSTLYDSWVNKLVFDGLITNNEKGEPIPNVAESYEVSEDGKTYTFKLNKGIKFTNGQELTAKDVAFTFTSICDP
GYDGPRMDAVNNLVGYEEYNKGDASSVEGIKVIDDYTISFTNKNTDAAGIWNFEYGIMPESVYKFEKGNFQAVKDKLLEP
VGSGAYKFVHFKPGQEVKFEKNADYWKGEPKIPYIVMKVTNAQTLLQELMAGTVDIDRVGAKPENIDPLKQAGFLNLDLY
MQNGYGYMGLNYGSDKVKDPKVRQALLYGLNREGFMQSYYQGYGQVYNSHILPTSWAYNPDVPKYEYNPEKAKELLDEAG
WKDTNGNGVRDKDGVELELQWLTYTGSKYVDALIPIVQQSWEQIGVKVTPELMEFGTMMDKVNNREYDIFNGAWNLSIDP
DPSGIFAISQDVPGGFNNIGWRNEEADKLLKEGKGTTNQEERKKAYAEWQLKFSEDVPYILLGNAQEMFASNSRVKGYNP
STYIDWTHDVYKLELDNNK
>Mature_579_residues
MKKRKLVALLTAGLAASMLFVACGGANNTAQGNGNGSESGGTTKDLSKPERIEASNPSALPDAAKNRTDTLIVGTTDPKG
EFVPIYSSTLYDSWVNKLVFDGLITNNEKGEPIPNVAESYEVSEDGKTYTFKLNKGIKFTNGQELTAKDVAFTFTSICDP
GYDGPRMDAVNNLVGYEEYNKGDASSVEGIKVIDDYTISFTNKNTDAAGIWNFEYGIMPESVYKFEKGNFQAVKDKLLEP
VGSGAYKFVHFKPGQEVKFEKNADYWKGEPKIPYIVMKVTNAQTLLQELMAGTVDIDRVGAKPENIDPLKQAGFLNLDLY
MQNGYGYMGLNYGSDKVKDPKVRQALLYGLNREGFMQSYYQGYGQVYNSHILPTSWAYNPDVPKYEYNPEKAKELLDEAG
WKDTNGNGVRDKDGVELELQWLTYTGSKYVDALIPIVQQSWEQIGVKVTPELMEFGTMMDKVNNREYDIFNGAWNLSIDP
DPSGIFAISQDVPGGFNNIGWRNEEADKLLKEGKGTTNQEERKKAYAEWQLKFSEDVPYILLGNAQEMFASNSRVKGYNP
STYIDWTHDVYKLELDNNK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=521, Percent_Identity=26.679462571977, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1787551, Length=482, Percent_Identity=24.6887966804979, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787052, Length=487, Percent_Identity=26.8993839835729, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789887, Length=484, Percent_Identity=23.7603305785124, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787762, Length=452, Percent_Identity=24.3362831858407, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1787495, Length=503, Percent_Identity=23.8568588469185, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081878, Length=494, Percent_Identity=22.8744939271255, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64657; Mature: 64657

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKLVALLTAGLAASMLFVACGGANNTAQGNGNGSESGGTTKDLSKPERIEASNPSAL
CCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDAAKNRTDTLIVGTTDPKGEFVPIYSSTLYDSWVNKLVFDGLITNNEKGEPIPNVAESY
CCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCEECCCCCCCCCCCHHHCC
EVSEDGKTYTFKLNKGIKFTNGQELTAKDVAFTFTSICDPGYDGPRMDAVNNLVGYEEYN
EECCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHCC
KGDASSVEGIKVIDDYTISFTNKNTDAAGIWNFEYGIMPESVYKFEKGNFQAVKDKLLEP
CCCCCCCCCEEEEECEEEEEECCCCCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHH
VGSGAYKFVHFKPGQEVKFEKNADYWKGEPKIPYIVMKVTNAQTLLQELMAGTVDIDRVG
CCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHCC
AKPENIDPLKQAGFLNLDLYMQNGYGYMGLNYGSDKVKDPKVRQALLYGLNREGFMQSYY
CCCCCCCHHHHCCEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
QGYGQVYNSHILPTSWAYNPDVPKYEYNPEKAKELLDEAGWKDTNGNGVRDKDGVELELQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
WLTYTGSKYVDALIPIVQQSWEQIGVKVTPELMEFGTMMDKVNNREYDIFNGAWNLSIDP
EEEECCHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCEEEECCEEEEEECC
DPSGIFAISQDVPGGFNNIGWRNEEADKLLKEGKGTTNQEERKKAYAEWQLKFSEDVPYI
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEEEEECCCCCEE
LLGNAQEMFASNSRVKGYNPSTYIDWTHDVYKLELDNNK
EECCHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCC
>Mature Secondary Structure
MKKRKLVALLTAGLAASMLFVACGGANNTAQGNGNGSESGGTTKDLSKPERIEASNPSAL
CCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDAAKNRTDTLIVGTTDPKGEFVPIYSSTLYDSWVNKLVFDGLITNNEKGEPIPNVAESY
CCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCEECCCCCCCCCCCHHHCC
EVSEDGKTYTFKLNKGIKFTNGQELTAKDVAFTFTSICDPGYDGPRMDAVNNLVGYEEYN
EECCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHCC
KGDASSVEGIKVIDDYTISFTNKNTDAAGIWNFEYGIMPESVYKFEKGNFQAVKDKLLEP
CCCCCCCCCEEEEECEEEEEECCCCCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHH
VGSGAYKFVHFKPGQEVKFEKNADYWKGEPKIPYIVMKVTNAQTLLQELMAGTVDIDRVG
CCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHCC
AKPENIDPLKQAGFLNLDLYMQNGYGYMGLNYGSDKVKDPKVRQALLYGLNREGFMQSYY
CCCCCCCHHHHCCEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
QGYGQVYNSHILPTSWAYNPDVPKYEYNPEKAKELLDEAGWKDTNGNGVRDKDGVELELQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
WLTYTGSKYVDALIPIVQQSWEQIGVKVTPELMEFGTMMDKVNNREYDIFNGAWNLSIDP
EEEECCHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCEEEECCEEEEEECC
DPSGIFAISQDVPGGFNNIGWRNEEADKLLKEGKGTTNQEERKKAYAEWQLKFSEDVPYI
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEEEEECCCCCEE
LLGNAQEMFASNSRVKGYNPSTYIDWTHDVYKLELDNNK
EECCHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]