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The map label for this gene is yclK [H]

Identifier: 18311221

GI number: 18311221

Start: 2561773

End: 2563188

Strand: Reverse

Name: yclK [H]

Synonym: CPE2239

Alternate gene names: 18311221

Gene position: 2563188-2561773 (Counterclockwise)

Preceding gene: 18311222

Following gene: 18311220

Centisome position: 84.55

GC content: 26.98

Gene sequence:

>1416_bases
ATGAAGAGTATAAAGACTAGGCTAATGAAAAACTTTTTAGTTCTTTTATTATCAACTATTATTATAGTTTCAGCCATGTT
TTTGATTTTTATTTCTAGATATTATTATCAAAATACAGAGGAAATATTATTAAGTCAAATTAATATATCTGTAGATTTTT
ATAAAAGATATTTATCAAATGTTAGTCTAGAAGAAAATGTTTATGAAGATGTAGATATATTTTGGAAACAAACAGATGCT
CAAGTTCAAATTTATAACTTAAAAGGGCAGTTAATAATGGATTCTATAGGTTTAGAGCCTAAAGAGTATAATACTCCTGT
AGATGTAAAAAGAGCCTTAGAAGGAGATACAGCAAAGTGGGTGGGGACTGTACCAGATTATACTGGTAAAGTAATGGCCA
TTTCAGCACCACTTAGAAATTCTTCAAATGAAATTGTAGGAGTTATTAGATATATAAGTTCACTTAGAAATGTGGATAAT
TTTATATTAAATTTCTTTTTAGTATTTTTAGTAATAGGATTAACAGTTTTAGCTATTGGTATAATTTTAAGTTACTTCTT
AGCTAATAGTATAGTAAATCCAATTACAGAATTAATAAAAGTTTCTGAACAAATGGCTAAGGGAAATTTAAAAGTAAGAA
ATAAGATTGTTACTAATGATGAAATTGAAAAGTTAGCTGATAGTCTTAATATAATGGCAGAGGAAATTGAAAATAGGGAA
ATATTAAAAAATGAATTTATTTCTTCAGTTTCTCATGAACTTAGAACACCATTAACATCAATAAAAGGTTGGGCAATAAC
TTTAAATAATGATTTTACAGATAGAGAAACCTTAAAGATGGGCTTTGACATAATTGAAAAAGAAGCTGATAGATTAAGTA
ATATGGTAGAGGAGCTATTAGACTTTTCAAAGTTTGTTAGCGGAAAAATAAAATTGAAATATGAAGAAATAAACCTAAAA
GAATTTATAGAGTACTTAAGGTTATATATGAATCCTAGGGCAGAAAGAGAACATAAAGAACTTATACTAAAAGGAGTTAC
AGAGGACTTTATAATTGTAGGAGATAAGGACCGATTAAAGCAAGTATTTATAAATATAATAGATAATGCTTTTAAATTCA
CTCATGAAAATGAAAAGATAACAATAGAGTTTGTATATGCTGATGAAGGAATCTATATAAACATAATTGATACTGGATGC
GGAATAAGTAAAGAAGAGTTACCTAGAGTAAAAGAGAAGTTTTACAAGGGTAAAAATTCAAAGTCACAAAATGGAATAGG
ATTATCAATATGTGATGAAATTATAGCTTTACATGAGGGGACTTTGGAGATTTATAGTGAATTAGGTAATGGAACGAAAG
TGGTTATATATTTACCTAAGAAATTAATCAGGAGTGTTGAGGATGATTTAAATTAA

Upstream 100 bases:

>100_bases
TCATAGGAATTTTATAAAGAGATATAAAGTATAGAGTGAATAATTAAAACTTATATTTTAAGTTTTTAGTATATATAGGT
GAAAGATAGGTGCATATTTT

Downstream 100 bases:

>100_bases
TCAAAAGAGGTGTTTTATGAAGAAAATTTTTTTAGTCTTCTTTACTATTATTAGCTTTTTTATGATGGGATGTTCTCAAA
AAGAATCTTTTGATATATCT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILLSQINISVDFYKRYLSNVSLEENVYEDVDIFWKQTDA
QVQIYNLKGQLIMDSIGLEPKEYNTPVDVKRALEGDTAKWVGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDN
FILNFFLVFLVIGLTVLAIGIILSYFLANSIVNPITELIKVSEQMAKGNLKVRNKIVTNDEIEKLADSLNIMAEEIENRE
ILKNEFISSVSHELRTPLTSIKGWAITLNNDFTDRETLKMGFDIIEKEADRLSNMVEELLDFSKFVSGKIKLKYEEINLK
EFIEYLRLYMNPRAEREHKELILKGVTEDFIIVGDKDRLKQVFINIIDNAFKFTHENEKITIEFVYADEGIYINIIDTGC
GISKEELPRVKEKFYKGKNSKSQNGIGLSICDEIIALHEGTLEIYSELGNGTKVVIYLPKKLIRSVEDDLN

Sequences:

>Translated_471_residues
MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILLSQINISVDFYKRYLSNVSLEENVYEDVDIFWKQTDA
QVQIYNLKGQLIMDSIGLEPKEYNTPVDVKRALEGDTAKWVGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDN
FILNFFLVFLVIGLTVLAIGIILSYFLANSIVNPITELIKVSEQMAKGNLKVRNKIVTNDEIEKLADSLNIMAEEIENRE
ILKNEFISSVSHELRTPLTSIKGWAITLNNDFTDRETLKMGFDIIEKEADRLSNMVEELLDFSKFVSGKIKLKYEEINLK
EFIEYLRLYMNPRAEREHKELILKGVTEDFIIVGDKDRLKQVFINIIDNAFKFTHENEKITIEFVYADEGIYINIIDTGC
GISKEELPRVKEKFYKGKNSKSQNGIGLSICDEIIALHEGTLEIYSELGNGTKVVIYLPKKLIRSVEDDLN
>Mature_471_residues
MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILLSQINISVDFYKRYLSNVSLEENVYEDVDIFWKQTDA
QVQIYNLKGQLIMDSIGLEPKEYNTPVDVKRALEGDTAKWVGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDN
FILNFFLVFLVIGLTVLAIGIILSYFLANSIVNPITELIKVSEQMAKGNLKVRNKIVTNDEIEKLADSLNIMAEEIENRE
ILKNEFISSVSHELRTPLTSIKGWAITLNNDFTDRETLKMGFDIIEKEADRLSNMVEELLDFSKFVSGKIKLKYEEINLK
EFIEYLRLYMNPRAEREHKELILKGVTEDFIIVGDKDRLKQVFINIIDNAFKFTHENEKITIEFVYADEGIYINIIDTGC
GISKEELPRVKEKFYKGKNSKSQNGIGLSICDEIIALHEGTLEIYSELGNGTKVVIYLPKKLIRSVEDDLN

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=278, Percent_Identity=25.1798561151079, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786600, Length=244, Percent_Identity=27.8688524590164, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=31.4516129032258, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786783, Length=297, Percent_Identity=26.5993265993266, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790436, Length=247, Percent_Identity=27.5303643724696, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=26.4822134387352, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=27.2058823529412, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789149, Length=333, Percent_Identity=26.1261261261261, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788549, Length=217, Percent_Identity=27.6497695852535, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=25.8333333333333, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=28.5087719298246, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790861, Length=368, Percent_Identity=22.8260869565217, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=288, Percent_Identity=24.6527777777778, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=23.6486486486486, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=295, Percent_Identity=21.6949152542373, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789403, Length=270, Percent_Identity=24.0740740740741, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=244, Percent_Identity=27.0491803278689, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787894, Length=291, Percent_Identity=23.7113402061856, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54080; Mature: 54080

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILLSQINISVDFYKRYLSN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCC
VSLEENVYEDVDIFWKQTDAQVQIYNLKGQLIMDSIGLEPKEYNTPVDVKRALEGDTAKW
CCHHHHHHHHHHHHEECCCCEEEEEECCCCEEEHHCCCCCCCCCCCHHHHHHHCCCCCCE
VGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDNFILNFFLVFLVIGLTVLAIG
EECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILSYFLANSIVNPITELIKVSEQMAKGNLKVRNKIVTNDEIEKLADSLNIMAEEIENRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHCCHH
ILKNEFISSVSHELRTPLTSIKGWAITLNNDFTDRETLKMGFDIIEKEADRLSNMVEELL
HHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DFSKFVSGKIKLKYEEINLKEFIEYLRLYMNPRAEREHKELILKGVTEDFIIVGDKDRLK
HHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHH
QVFINIIDNAFKFTHENEKITIEFVYADEGIYINIIDTGCGISKEELPRVKEKFYKGKNS
HHHHHHHHHHHHEECCCCEEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCC
KSQNGIGLSICDEIIALHEGTLEIYSELGNGTKVVIYLPKKLIRSVEDDLN
CCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure
MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILLSQINISVDFYKRYLSN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCC
VSLEENVYEDVDIFWKQTDAQVQIYNLKGQLIMDSIGLEPKEYNTPVDVKRALEGDTAKW
CCHHHHHHHHHHHHEECCCCEEEEEECCCCEEEHHCCCCCCCCCCCHHHHHHHCCCCCCE
VGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDNFILNFFLVFLVIGLTVLAIG
EECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILSYFLANSIVNPITELIKVSEQMAKGNLKVRNKIVTNDEIEKLADSLNIMAEEIENRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHHHHHHHCCHH
ILKNEFISSVSHELRTPLTSIKGWAITLNNDFTDRETLKMGFDIIEKEADRLSNMVEELL
HHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DFSKFVSGKIKLKYEEINLKEFIEYLRLYMNPRAEREHKELILKGVTEDFIIVGDKDRLK
HHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCHHHHH
QVFINIIDNAFKFTHENEKITIEFVYADEGIYINIIDTGCGISKEELPRVKEKFYKGKNS
HHHHHHHHHHHHEECCCCEEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCC
KSQNGIGLSICDEIIALHEGTLEIYSELGNGTKVVIYLPKKLIRSVEDDLN
CCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]