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The map label for this gene is emrB [H]

Identifier: 17547446

GI number: 17547446

Start: 2933144

End: 2934679

Strand: Direct

Name: emrB [H]

Synonym: RSc2727

Alternate gene names: 17547446

Gene position: 2933144-2934679 (Clockwise)

Preceding gene: 17547445

Following gene: 17547449

Centisome position: 78.92

GC content: 70.9

Gene sequence:

>1536_bases
GTGACGGCGAACGGATCGCGGGCGGATGCCGCGGCCTGGGCGGCCGTCGCGGCCGGCACCCTCGGCGCCATGATGGCCAC
GCTCGACATCTCCATCGTGAATTCGGCGCTGCCGACCATCCAGGGCGAAATCGGCGCCACCAGCACCGAAGGCACGTGGA
TCGCCACGGCCTACCTAGTCGCGGAAATCATCATCATCCCCCTGGCCGGATGGCTGGAGAAACTGCTCGGACTGCGCAAC
CTGCTGCTGGCGGCCACCGGGCTCTTCACCGGGTTCTCGATGCTGTGCGGCGTCGCCGAGACCTTGCCGATGATGGTGAT
CGGGCGGGCGGGCCAGGGGTTCACCGGGGGCGTGCTGATCCCCACCGCGATGACCATCATCGCCGCGCGGCTGCCCCGCG
CGCAGCAGCCGCTGGGCACCGCGCTGTTCGGCTCGACCGTCATCCTCGGGCCGGTCTTCGGCCCCATGCTCGGCGGCTGG
ATGACCACGCAGCTCAACTGGCACTACGCGTTCTTCATCAACCTGCCGATCTGCGGGGTGCTGGCCGCGCTGCTCCTGCT
CGGCATGCCCCATGAGCGCCCCCGCACGGCGCTGCTGCGGGAGGCGGACTGGGCGGGCATCGCCGGCCTCGCCCTCGGCC
TGGGCGGCATGACGGTGGTGCTGGAGGAAGGCCAGCGCCTGCAATGGTTCACCTCCCCTGCGATCCGCTGGCTGGCGGCC
GTGTCGGCGCTCGGCTTTGGCCTGCTGGGCTGGGGCCAGGCGCGCGCCCGCGCCCCGGTCATCCGCCTCGGGCTGCTGCG
GGACCGGCAGTTCGGCGCCATCGCGGTCATGGCCATCGCGGCCAGCATGGCGTTGTACGGCACCGCCTACGTGATCCCCC
AGTTCCTGGTCGGCATCGCGGGCTACGACGCACTGCAATCGGGCTGGATCGTCCTGCTGTCGGGGCTGCCGATGATCGTG
CTGATGCCGATGCTGCCCCTGATGCTGCGCTGGCTCGACGTCCGGCTGGCGGTCGGCGGCGGCCTGCTGGTGCTGGCGCT
AAGCGCCTACATCGAGACCGGGCTGAGCCCCCTGTCGACGGGCAACTCGTTCGTGGCCTCGCAGCTGATGCGCGGGCTCG
GCACCGTGCTGACCATGATGTTCCTGAACCAGGCCGCCATCCGCTCCGTGCCGCCGTCGCAGGCGAGCGATGCCTCCGGG
CTCTACAACGGCCTGCGCAACCTGGGCGGCTCGATCGCGCTGGCCTGCATCGCCACGCTGCAGGAGCAGCGGCTCTGGCT
GCACAGCCGCCGCATCGAGGACACCCTGCAGGCCAACGCGGCCGGCGTGCAGGATTACATCGCCGGCCAGGCCCACGCGC
TGGGCAGCCAGGCCGCCGCCCTGCTGTCGATGGAGCAGTCCATCCAGGTGCAGGCGCTGACGATGGCGTATGCCGACCTG
TTCTGGCTGCTGACCGTGGCAATCCTGCTGGTGACGCCGCTGGTGGTCTTCCTGCGGCCCCTGCCCCGCCATGCCGGCCC
GGTTGCCGGGCATTGA

Upstream 100 bases:

>100_bases
AGCCGCTGCTGGCGGCCGGCATGTCGGTCACGGTCGATGTCGATACGCTCGCGCCGGTCCGGCCCGCGGCGCACCCGCAG
CCGGGCAGCGGAGCCCTTCT

Downstream 100 bases:

>100_bases
TTCAGAGATAGCGCATCCAGGGGTGGCGCCCGGTCCGGCGCAACGCGGCGAACCTGCGCGTCGGGCCATACAGCAGCGCC
ACGGTGGGCAGCCACGCGGC

Product: multidrug resistance transmembrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTANGSRADAAAWAAVAAGTLGAMMATLDISIVNSALPTIQGEIGATSTEGTWIATAYLVAEIIIIPLAGWLEKLLGLRN
LLLAATGLFTGFSMLCGVAETLPMMVIGRAGQGFTGGVLIPTAMTIIAARLPRAQQPLGTALFGSTVILGPVFGPMLGGW
MTTQLNWHYAFFINLPICGVLAALLLLGMPHERPRTALLREADWAGIAGLALGLGGMTVVLEEGQRLQWFTSPAIRWLAA
VSALGFGLLGWGQARARAPVIRLGLLRDRQFGAIAVMAIAASMALYGTAYVIPQFLVGIAGYDALQSGWIVLLSGLPMIV
LMPMLPLMLRWLDVRLAVGGGLLVLALSAYIETGLSPLSTGNSFVASQLMRGLGTVLTMMFLNQAAIRSVPPSQASDASG
LYNGLRNLGGSIALACIATLQEQRLWLHSRRIEDTLQANAAGVQDYIAGQAHALGSQAAALLSMEQSIQVQALTMAYADL
FWLLTVAILLVTPLVVFLRPLPRHAGPVAGH

Sequences:

>Translated_511_residues
MTANGSRADAAAWAAVAAGTLGAMMATLDISIVNSALPTIQGEIGATSTEGTWIATAYLVAEIIIIPLAGWLEKLLGLRN
LLLAATGLFTGFSMLCGVAETLPMMVIGRAGQGFTGGVLIPTAMTIIAARLPRAQQPLGTALFGSTVILGPVFGPMLGGW
MTTQLNWHYAFFINLPICGVLAALLLLGMPHERPRTALLREADWAGIAGLALGLGGMTVVLEEGQRLQWFTSPAIRWLAA
VSALGFGLLGWGQARARAPVIRLGLLRDRQFGAIAVMAIAASMALYGTAYVIPQFLVGIAGYDALQSGWIVLLSGLPMIV
LMPMLPLMLRWLDVRLAVGGGLLVLALSAYIETGLSPLSTGNSFVASQLMRGLGTVLTMMFLNQAAIRSVPPSQASDASG
LYNGLRNLGGSIALACIATLQEQRLWLHSRRIEDTLQANAAGVQDYIAGQAHALGSQAAALLSMEQSIQVQALTMAYADL
FWLLTVAILLVTPLVVFLRPLPRHAGPVAGH
>Mature_510_residues
TANGSRADAAAWAAVAAGTLGAMMATLDISIVNSALPTIQGEIGATSTEGTWIATAYLVAEIIIIPLAGWLEKLLGLRNL
LLAATGLFTGFSMLCGVAETLPMMVIGRAGQGFTGGVLIPTAMTIIAARLPRAQQPLGTALFGSTVILGPVFGPMLGGWM
TTQLNWHYAFFINLPICGVLAALLLLGMPHERPRTALLREADWAGIAGLALGLGGMTVVLEEGQRLQWFTSPAIRWLAAV
SALGFGLLGWGQARARAPVIRLGLLRDRQFGAIAVMAIAASMALYGTAYVIPQFLVGIAGYDALQSGWIVLLSGLPMIVL
MPMLPLMLRWLDVRLAVGGGLLVLALSAYIETGLSPLSTGNSFVASQLMRGLGTVLTMMFLNQAAIRSVPPSQASDASGL
YNGLRNLGGSIALACIATLQEQRLWLHSRRIEDTLQANAAGVQDYIAGQAHALGSQAAALLSMEQSIQVQALTMAYADLF
WLLTVAILLVTPLVVFLRPLPRHAGPVAGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=486, Percent_Identity=31.0699588477366, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788710, Length=495, Percent_Identity=29.0909090909091, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1790195, Length=328, Percent_Identity=28.9634146341463, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788392, Length=210, Percent_Identity=29.5238095238095, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081983, Length=416, Percent_Identity=24.0384615384615, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=192, Percent_Identity=26.0416666666667, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=274, Percent_Identity=25.5474452554745, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53693; Mature: 53562

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANGSRADAAAWAAVAAGTLGAMMATLDISIVNSALPTIQGEIGATSTEGTWIATAYLV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AEIIIIPLAGWLEKLLGLRNLLLAATGLFTGFSMLCGVAETLPMMVIGRAGQGFTGGVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHH
PTAMTIIAARLPRAQQPLGTALFGSTVILGPVFGPMLGGWMTTQLNWHYAFFINLPICGV
HHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHCCEEEEEECEEEEEEECCCHHHH
LAALLLLGMPHERPRTALLREADWAGIAGLALGLGGMTVVLEEGQRLQWFTSPAIRWLAA
HHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCEEEHHHHHHHHHHH
VSALGFGLLGWGQARARAPVIRLGLLRDRQFGAIAVMAIAASMALYGTAYVIPQFLVGIA
HHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYDALQSGWIVLLSGLPMIVLMPMLPLMLRWLDVRLAVGGGLLVLALSAYIETGLSPLST
HHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
GNSFVASQLMRGLGTVLTMMFLNQAAIRSVPPSQASDASGLYNGLRNLGGSIALACIATL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
QEQRLWLHSRRIEDTLQANAAGVQDYIAGQAHALGSQAAALLSMEQSIQVQALTMAYADL
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWLLTVAILLVTPLVVFLRPLPRHAGPVAGH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TANGSRADAAAWAAVAAGTLGAMMATLDISIVNSALPTIQGEIGATSTEGTWIATAYLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AEIIIIPLAGWLEKLLGLRNLLLAATGLFTGFSMLCGVAETLPMMVIGRAGQGFTGGVLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHH
PTAMTIIAARLPRAQQPLGTALFGSTVILGPVFGPMLGGWMTTQLNWHYAFFINLPICGV
HHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHCCEEEEEECEEEEEEECCCHHHH
LAALLLLGMPHERPRTALLREADWAGIAGLALGLGGMTVVLEEGQRLQWFTSPAIRWLAA
HHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCEEEHHHHHHHHHHH
VSALGFGLLGWGQARARAPVIRLGLLRDRQFGAIAVMAIAASMALYGTAYVIPQFLVGIA
HHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYDALQSGWIVLLSGLPMIVLMPMLPLMLRWLDVRLAVGGGLLVLALSAYIETGLSPLST
HHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
GNSFVASQLMRGLGTVLTMMFLNQAAIRSVPPSQASDASGLYNGLRNLGGSIALACIATL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHH
QEQRLWLHSRRIEDTLQANAAGVQDYIAGQAHALGSQAAALLSMEQSIQVQALTMAYADL
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWLLTVAILLVTPLVVFLRPLPRHAGPVAGH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]