BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is kduD [C]

Identifier: 17546449

GI number: 17546449

Start: 1851224

End: 1851994

Strand: Direct

Name: kduD [C]

Synonym: RSc1730

Alternate gene names: 17546449

Gene position: 1851224-1851994 (Clockwise)

Preceding gene: 17546447

Following gene: 17546456

Centisome position: 49.81

GC content: 69.13

Gene sequence:

>771_bases
ATGACCCAACCCCAATCCCCCACCCCCATCGCCCTGGTCACCGGCGGCAGCCGCGGCCTCGGCAGGAACATGGTGCAGAA
GCTGGCCCGCGGCGGCACCGATGTGATCTTCACCTACCGCAGCAACCAGGCCGAGGCGCAGGCGCTGGTCGCGGAACTGG
CCGCGCTCGGACGCCGCGCCGCCGCGCTGCAACTGGACGCCGGCCAGATCGCCACCTTCGCCGCATTCGCCGATGCGGTG
CGCGGCGTGCTGGCGCAATGGCAGCGCGAGCGCATCGACGCGCTCGTCAACAATGCCGGCATCGGCATCCACGCCAGTCT
GGCCGAGATGACCGAAGCGCAGTTCGATGCGCTGATGAACGTGCACTTCAAGGGCGTGTTTTTCCTGACGCAGGCGCTGC
TGCCGCTGATTGCCGATGGCGGGCGCATCGTCAACATTTCGTCGGGGCTGACGCGCTTTGCCTTCGTGGGCTACGGCGCC
TACGCGGCGATGAAGGGCGCGGTCGAGGTGCTGACGAAATACATGGCGAAGGAACTCGGCCCGCGCGGCATTGCGGTGAA
CGTGGTGGCGCCGGGGGCGATCGAGACCGACTTCGGCGGTGGCCGGGTGCGCGATGACGCAAACCTGAACGCCATGGTGG
CCGCGCAGACGGCGCTGGGCCGCGTGGGCCTGCCGGACGATATCGGCGGGGTGGTGGCGTCGCTGCTGGCGCCGGACAAC
CGCTGGATCAACGCGCAGCGGATCGAGGCGTCGGGCGGGATGTTCCTCTAG

Upstream 100 bases:

>100_bases
AAAACACAGCAATAGAATAATCGAGATTTATTCCGTGTTTGTCGCCCAATAACATGCAGGTGTTCACGGTGCCCGGCACC
CGCCATCACAGGAGCGCGAC

Downstream 100 bases:

>100_bases
GCCCTTCAGGCCCTTCAGACCGGCCCGACGCCGGCGGCGGTGGCGCGTTCAGGCCACCGCCTTGGCCGCGGCCCGGCCGC
GCAGCCATTCGAAGGTCAGC

Product: dehydrogenase/reductase oxidoreductase

Products: @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate!; NADH. [C]

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTQPQSPTPIALVTGGSRGLGRNMVQKLARGGTDVIFTYRSNQAEAQALVAELAALGRRAAALQLDAGQIATFAAFADAV
RGVLAQWQRERIDALVNNAGIGIHASLAEMTEAQFDALMNVHFKGVFFLTQALLPLIADGGRIVNISSGLTRFAFVGYGA
YAAMKGAVEVLTKYMAKELGPRGIAVNVVAPGAIETDFGGGRVRDDANLNAMVAAQTALGRVGLPDDIGGVVASLLAPDN
RWINAQRIEASGGMFL

Sequences:

>Translated_256_residues
MTQPQSPTPIALVTGGSRGLGRNMVQKLARGGTDVIFTYRSNQAEAQALVAELAALGRRAAALQLDAGQIATFAAFADAV
RGVLAQWQRERIDALVNNAGIGIHASLAEMTEAQFDALMNVHFKGVFFLTQALLPLIADGGRIVNISSGLTRFAFVGYGA
YAAMKGAVEVLTKYMAKELGPRGIAVNVVAPGAIETDFGGGRVRDDANLNAMVAAQTALGRVGLPDDIGGVVASLLAPDN
RWINAQRIEASGGMFL
>Mature_255_residues
TQPQSPTPIALVTGGSRGLGRNMVQKLARGGTDVIFTYRSNQAEAQALVAELAALGRRAAALQLDAGQIATFAAFADAVR
GVLAQWQRERIDALVNNAGIGIHASLAEMTEAQFDALMNVHFKGVFFLTQALLPLIADGGRIVNISSGLTRFAFVGYGAY
AAMKGAVEVLTKYMAKELGPRGIAVNVVAPGAIETDFGGGRVRDDANLNAMVAAQTALGRVGLPDDIGGVVASLLAPDNR
WINAQRIEASGGMFL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=33.0769230769231, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI32483357, Length=248, Percent_Identity=30.241935483871, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI5031737, Length=247, Percent_Identity=29.1497975708502, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI7705925, Length=216, Percent_Identity=32.4074074074074, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4502599, Length=286, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI119392083, Length=191, Percent_Identity=30.8900523560209, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI10190704, Length=251, Percent_Identity=28.2868525896414, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI33667109, Length=193, Percent_Identity=30.5699481865285, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI4503817, Length=202, Percent_Identity=25.2475247524752, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI59889578, Length=187, Percent_Identity=31.0160427807487, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI22507405, Length=119, Percent_Identity=35.2941176470588, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI150247226, Length=192, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI66933014, Length=236, Percent_Identity=26.6949152542373, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI20149619, Length=219, Percent_Identity=27.8538812785388, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=33.7349397590361, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=34, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI2367365, Length=236, Percent_Identity=31.7796610169492, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=31.6, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI2367175, Length=254, Percent_Identity=31.496062992126, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=31.8897637795276, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=30.0395256916996, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=27.5303643724696, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=27.2, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787905, Length=246, Percent_Identity=29.6747967479675, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786701, Length=222, Percent_Identity=30.1801801801802, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787820, Length=229, Percent_Identity=28.82096069869, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787526, Length=190, Percent_Identity=32.6315789473684, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=29.6442687747036, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=252, Percent_Identity=30.1587301587302, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994604, Length=251, Percent_Identity=30.2788844621514, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=260, Percent_Identity=29.2307692307692, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=30.078125, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=30.7086614173228, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=29.6, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=247, Percent_Identity=27.1255060728745, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=246, Percent_Identity=30.0813008130081, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=29.3893129770992, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=31.2267657992565, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=30.5343511450382, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=196, Percent_Identity=31.6326530612245, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17544670, Length=239, Percent_Identity=27.6150627615063, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=27.8810408921933, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=258, Percent_Identity=29.0697674418605, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=260, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=193, Percent_Identity=28.4974093264249, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=260, Percent_Identity=29.6153846153846, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558990, Length=221, Percent_Identity=28.5067873303167, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=196, Percent_Identity=32.6530612244898, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=264, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=276, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=263, Percent_Identity=27.3764258555133, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=196, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=24.4979919678715, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=34.9206349206349, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=35.3413654618474, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI23397609, Length=231, Percent_Identity=34.1991341991342, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=29.0322580645161, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571526, Length=248, Percent_Identity=32.6612903225806, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21358495, Length=225, Percent_Identity=31.5555555555556, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=32.2834645669291, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24644339, Length=233, Percent_Identity=29.6137339055794, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651139, Length=193, Percent_Identity=29.5336787564767, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644337, Length=167, Percent_Identity=32.9341317365269, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656154, Length=215, Percent_Identity=29.3023255813954, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI19922628, Length=215, Percent_Identity=29.3023255813954, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125 [C]

Molecular weight: Translated: 26767; Mature: 26636

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPQSPTPIALVTGGSRGLGRNMVQKLARGGTDVIFTYRSNQAEAQALVAELAALGRRA
CCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCHH
AALQLDAGQIATFAAFADAVRGVLAQWQRERIDALVNNAGIGIHASLAEMTEAQFDALMN
HEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHH
VHFKGVFFLTQALLPLIADGGRIVNISSGLTRFAFVGYGAYAAMKGAVEVLTKYMAKELG
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRGIAVNVVAPGAIETDFGGGRVRDDANLNAMVAAQTALGRVGLPDDIGGVVASLLAPDN
CCCEEEEEECCCCEEECCCCCEECCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHCCCC
RWINAQRIEASGGMFL
CCCCCEEEECCCCCCC
>Mature Secondary Structure 
TQPQSPTPIALVTGGSRGLGRNMVQKLARGGTDVIFTYRSNQAEAQALVAELAALGRRA
CCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCHH
AALQLDAGQIATFAAFADAVRGVLAQWQRERIDALVNNAGIGIHASLAEMTEAQFDALMN
HEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHH
VHFKGVFFLTQALLPLIADGGRIVNISSGLTRFAFVGYGAYAAMKGAVEVLTKYMAKELG
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRGIAVNVVAPGAIETDFGGGRVRDDANLNAMVAAQTALGRVGLPDDIGGVVASLLAPDN
CCCEEEEEECCCCEEECCCCCEECCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHCCCC
RWINAQRIEASGGMFL
CCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 15.4 {2-deoxygluconate}} [C]

Substrates: @DEOX03.txt*2-Deoxy-D-Gluconate!; NAD(+) [C]

Specific reaction: @DEOX03.txt*2-Deoxy-D-Gluconate! + NAD(+) = @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]