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The map label for this gene is dusC

Identifier: 17546354

GI number: 17546354

Start: 1756422

End: 1757402

Strand: Direct

Name: dusC

Synonym: RSc1635

Alternate gene names: 17546354

Gene position: 1756422-1757402 (Clockwise)

Preceding gene: 17546353

Following gene: 17546355

Centisome position: 47.26

GC content: 70.85

Gene sequence:

>981_bases
ATGAGCCGGCTCTTGCTCGCCCCGATGGAAGGGGTTGCGGACTTTGTCATGCGCGACGTGCTGACCAGCGTCGGCGGCTA
CGACGGATGCGTGTCCGAGTTCGTCCGGGTGACGGGCTCGTTGCTTCCCGCGCGCACCTACGAGCGGGAAACCCCGGAAA
TCCGCAACGGCGGCTACACCGCCAGCGGCACGCCGATGGTGATCCAGCTGCTCGGCAGCGACCCGGAGTGGCTGGCCCGG
AACGCCGCGCAGGCCGCCACCGTCTCGCCGCATGGCATCGACCTGAATTTCGGGTGTCCGGCCAAGGTCGTCAACCGGCA
CGGCGGCGGCGCCATGCTGCTGGCCACGCCCGAGCTGCTGCACCGGATCGTGTCCACCGTGCGTGCCGCGGTTCCCGCCC
GGATTGCCGTGACCGCCAAAATGCGGCTCGGCGTCTCCGATACCTCGCTGGCCATCGCATGCGCGACCGCGCTGGCGGAG
GGCGGGGCGGCTTCGCTGGTCGTGCATGCCCGCACGCGTGACCACGGCTACCGGCCGCCGGCCCACTGGGACTGGATCGC
GCGGATTGCCGATGCCGTGCGCGTGCCCGTGGTCGCCAACGGCGAAGTCTGGACCGTCGACGATTGGGCGCGGTGCCGGG
CGGTGAGCGGCTGCGACGACGTGATGATCGGGCGGGGCGCGGTGTCCGATCCGTTCCTCGCCCTGCGCATCCGCGGGCAG
ATGGCACGGCAGCCGTCCGACGCGGAATGGCCGCTCGTGCTGGGCTGCCTCGCCGATTACCTCAAGAAGCTGCGCGCGCG
CATCGCCATCCACCACGAGCATGGCCGCGTCAAGCTGTGGCTCGGCTACCTGAAGCGGACCTGGCCGCAGGCGGCGGAGC
TGCACGACGCGATCCGCCGTCTGCAGGACTCCGCGGAGATCCTGGGCGTGATCGAACATGCGCTGGCCCGGATTGGCCAG
CAGAGCGCGCCGGCAGGGTAA

Upstream 100 bases:

>100_bases
GCGTGGCGCCCGTTCCCGCAGATCCTTGTCTCCTCTTCACGGTTGTCACCATGTCCGACGTCAGCGTCCGGCGTCAGCGC
CGGCCGGCATTCGCTTTCGC

Downstream 100 bases:

>100_bases
AATGCGCATCCGCGCCGCTGCCATGCGCGCGCGGCACAGATCCTCTCCCCACATCAGGTTGTTCCCATGTCCGACGTCAG
TACCCAGAGCCCCAAGGTGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSRLLLAPMEGVADFVMRDVLTSVGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMVIQLLGSDPEWLAR
NAAQAATVSPHGIDLNFGCPAKVVNRHGGGAMLLATPELLHRIVSTVRAAVPARIAVTAKMRLGVSDTSLAIACATALAE
GGAASLVVHARTRDHGYRPPAHWDWIARIADAVRVPVVANGEVWTVDDWARCRAVSGCDDVMIGRGAVSDPFLALRIRGQ
MARQPSDAEWPLVLGCLADYLKKLRARIAIHHEHGRVKLWLGYLKRTWPQAAELHDAIRRLQDSAEILGVIEHALARIGQ
QSAPAG

Sequences:

>Translated_326_residues
MSRLLLAPMEGVADFVMRDVLTSVGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMVIQLLGSDPEWLAR
NAAQAATVSPHGIDLNFGCPAKVVNRHGGGAMLLATPELLHRIVSTVRAAVPARIAVTAKMRLGVSDTSLAIACATALAE
GGAASLVVHARTRDHGYRPPAHWDWIARIADAVRVPVVANGEVWTVDDWARCRAVSGCDDVMIGRGAVSDPFLALRIRGQ
MARQPSDAEWPLVLGCLADYLKKLRARIAIHHEHGRVKLWLGYLKRTWPQAAELHDAIRRLQDSAEILGVIEHALARIGQ
QSAPAG
>Mature_325_residues
SRLLLAPMEGVADFVMRDVLTSVGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYTASGTPMVIQLLGSDPEWLARN
AAQAATVSPHGIDLNFGCPAKVVNRHGGGAMLLATPELLHRIVSTVRAAVPARIAVTAKMRLGVSDTSLAIACATALAEG
GAASLVVHARTRDHGYRPPAHWDWIARIADAVRVPVVANGEVWTVDDWARCRAVSGCDDVMIGRGAVSDPFLALRIRGQM
ARQPSDAEWPLVLGCLADYLKKLRARIAIHHEHGRVKLWLGYLKRTWPQAAELHDAIRRLQDSAEILGVIEHALARIGQQ
SAPAG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=180, Percent_Identity=30, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI40807366, Length=237, Percent_Identity=27.4261603375527, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI239788483, Length=196, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI239788462, Length=196, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI8923374, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=43.2692307692308, Blast_Score=257, Evalue=9e-70,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=31.9502074688797, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI145693211, Length=219, Percent_Identity=27.3972602739726, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=171, Percent_Identity=30.9941520467836, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=175, Percent_Identity=32.5714285714286, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=176, Percent_Identity=26.1363636363636, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=171, Percent_Identity=29.8245614035088, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=204, Percent_Identity=26.9607843137255, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=201, Percent_Identity=28.3582089552239, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585320, Length=199, Percent_Identity=32.6633165829146, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921524, Length=176, Percent_Identity=30.1136363636364, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45549423, Length=139, Percent_Identity=35.9712230215827, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSC_RALSO (Q8XYX1)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519756.1
- ProteinModelPortal:   Q8XYX1
- SMR:   Q8XYX1
- GeneID:   1220470
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1635
- NMPDR:   fig|267608.1.peg.1635
- HOGENOM:   HBG557545
- OMA:   CHEHGRV
- ProtClustDB:   CLSK629969
- BioCyc:   RSOL267608:RSC1635-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: NA

Molecular weight: Translated: 35144; Mature: 35013

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLLAPMEGVADFVMRDVLTSVGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYT
CCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
ASGTPMVIQLLGSDPEWLARNAAQAATVSPHGIDLNFGCPAKVVNRHGGGAMLLATPELL
CCCCCEEEEEECCCHHHHHHCCHHHCCCCCCCEEECCCCCHHHHHCCCCCEEEEECHHHH
HRIVSTVRAAVPARIAVTAKMRLGVSDTSLAIACATALAEGGAASLVVHARTRDHGYRPP
HHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AHWDWIARIADAVRVPVVANGEVWTVDDWARCRAVSGCDDVMIGRGAVSDPFLALRIRGQ
CHHHHHHHHHHHHCCCEEECCCEEECCHHHHHHHCCCCCCCEECCCCCCCCEEEEEECHH
MARQPSDAEWPLVLGCLADYLKKLRARIAIHHEHGRVKLWLGYLKRTWPQAAELHDAIRR
HHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEEHHHHHHHCCHHHHHHHHHHH
LQDSAEILGVIEHALARIGQQSAPAG
HHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRLLLAPMEGVADFVMRDVLTSVGGYDGCVSEFVRVTGSLLPARTYERETPEIRNGGYT
CCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
ASGTPMVIQLLGSDPEWLARNAAQAATVSPHGIDLNFGCPAKVVNRHGGGAMLLATPELL
CCCCCEEEEEECCCHHHHHHCCHHHCCCCCCCEEECCCCCHHHHHCCCCCEEEEECHHHH
HRIVSTVRAAVPARIAVTAKMRLGVSDTSLAIACATALAEGGAASLVVHARTRDHGYRPP
HHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AHWDWIARIADAVRVPVVANGEVWTVDDWARCRAVSGCDDVMIGRGAVSDPFLALRIRGQ
CHHHHHHHHHHHHCCCEEECCCEEECCHHHHHHHCCCCCCCEECCCCCCCCEEEEEECHH
MARQPSDAEWPLVLGCLADYLKKLRARIAIHHEHGRVKLWLGYLKRTWPQAAELHDAIRR
HHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEEHHHHHHHCCHHHHHHHHHHH
LQDSAEILGVIEHALARIGQQSAPAG
HHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852