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The map label for this gene is rimO [H]

Identifier: 17546355

GI number: 17546355

Start: 1757469

End: 1758830

Strand: Direct

Name: rimO [H]

Synonym: RSc1636

Alternate gene names: 17546355

Gene position: 1757469-1758830 (Clockwise)

Preceding gene: 17546354

Following gene: 17546356

Centisome position: 47.29

GC content: 66.67

Gene sequence:

>1362_bases
ATGTCCGACGTCAGTACCCAGAGCCCCAAGGTGGGGTTCGTCAGCCTCGGTTGTCCCAAAGCCCTCGTGGATTCCGAGCA
GATCATCACCCAGCTGCGCGCCGAGGGGTACGAGATTTCCGGCACCTATGGCGGGGCCGACCTGGTGGTCGTGAACACCT
GCGGTTTCATCGATGAGGCCGTGCAGGAAAGCCTGGACGCCATCGGCGAGGCGCTGGCCGAGAACGGCAAGGTGATCGTC
ACCGGCTGCCTGGGCGCGAAGAAGGACGCCGCCGGCCAGGACATCATCACCAGCGTGCATCCCAAGGTGCTGGCCGTGAC
CGGTCCGCATGCGCTGGGCGAGGTGATGGAGGCGGTACATACGCACCTGCCCAAGCCGCACGATCCGTTCATCGACCTGG
TGCCGCCGCAGGGCATCAAGCTCACGCCGAAGCACTACGCGTATCTGAAGATTTCCGAAGGCTGCAATCACCGCTGCAGC
TTCTGCATCATCCCGTCGATGCGCGGCGACCTGGTCTCGCGCCCGGTGGCCGAAGTGATGCTGGAGGCGGAGAACCTGCT
GAAGGCCGGCGTGAAGGAGCTGCTGGTAATCTCGCAGGACACCAGCGCCTACGGCGTCGACGTCAAGTTCCGTACCGGCT
TCTGGAACGGCCGGCCGCTCAAGACGCGCATGACCGAACTGGTCGGCGCGCTGGGCGAACTGGCGGCCCAGTATGGCGCC
TGGGTGCGCCTGCATTACGTCTACCCGTATCCGAGCGTGGACGAGGTGATGCCGCTGATGGCCGAGGGCAAGGTGCTGCC
GTATCTCGACGTGCCGCTGCAGCATGCCCATCCGGAAGTGCTCAAGCGCATGAAGCGCCCGGCCAACGCGGAGAAGACGC
TGGACCGCATCCGCGCCTGGCGCGAGGTCTGCCCGGAGCTGACCATCCGCAGCACCTTCATCGCCGGGTTCCCGGGCGAG
ACGGAGGAAGAGTTCCAGACGCTGCTCGACTTCATCGCCGAGGCCGAGCTCGATCGTGTCGGTTGCTTCGCCTACTCCCC
GGTGGAGGGCGCGACCGCCAACGATCTGCCGGGCGCGCTGCCCGACGAGGTGCGCGAGGAGCGCCGCGCGCGCTTCATGG
AAGTGGCCGAGCGCGTGTCGGCCCGCCGCCTGCAGCGCAAAGTGGGCAAGACGCTGCGCGTGCTGGTGGATGAGGTCAAC
CAGGATGGCGGCATCGGCCGCTCGTCGGCGGATGCGCCGGAGATCGACGGCCTGGTCTACATTGCGCCGCCGTCCAAGCC
GTACAAGCGCTACAAGGCGGGCGATTTCGTGTCGGTGAAGATCACCGGCGCCGATGGCCACGACCTGTGGGGCGAGGTCT
GA

Upstream 100 bases:

>100_bases
CCGGATTGGCCAGCAGAGCGCGCCGGCAGGGTAAAATGCGCATCCGCGCCGCTGCCATGCGCGCGCGGCACAGATCCTCT
CCCCACATCAGGTTGTTCCC

Downstream 100 bases:

>100_bases
TCCGTCGAGGCGGGCCTCCAGCAGGCCAGGCCATTTTCGAACGATCGTTTGCTTATACTGGTTGCCATTGACGATCAATT
GCCTTGGAGACAGCAATGGC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEAVQESLDAIGEALAENGKVIV
TGCLGAKKDAAGQDIITSVHPKVLAVTGPHALGEVMEAVHTHLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCS
FCIIPSMRGDLVSRPVAEVMLEAENLLKAGVKELLVISQDTSAYGVDVKFRTGFWNGRPLKTRMTELVGALGELAAQYGA
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPEVLKRMKRPANAEKTLDRIRAWREVCPELTIRSTFIAGFPGE
TEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAERVSARRLQRKVGKTLRVLVDEVN
QDGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV

Sequences:

>Translated_453_residues
MSDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEAVQESLDAIGEALAENGKVIV
TGCLGAKKDAAGQDIITSVHPKVLAVTGPHALGEVMEAVHTHLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCS
FCIIPSMRGDLVSRPVAEVMLEAENLLKAGVKELLVISQDTSAYGVDVKFRTGFWNGRPLKTRMTELVGALGELAAQYGA
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPEVLKRMKRPANAEKTLDRIRAWREVCPELTIRSTFIAGFPGE
TEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAERVSARRLQRKVGKTLRVLVDEVN
QDGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV
>Mature_452_residues
SDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEAVQESLDAIGEALAENGKVIVT
GCLGAKKDAAGQDIITSVHPKVLAVTGPHALGEVMEAVHTHLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCSF
CIIPSMRGDLVSRPVAEVMLEAENLLKAGVKELLVISQDTSAYGVDVKFRTGFWNGRPLKTRMTELVGALGELAAQYGAW
VRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPEVLKRMKRPANAEKTLDRIRAWREVCPELTIRSTFIAGFPGET
EEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAERVSARRLQRKVGKTLRVLVDEVNQ
DGGIGRSSADAPEIDGLVYIAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=488, Percent_Identity=25.6147540983607, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=25.6147540983607, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI93277076, Length=411, Percent_Identity=27.007299270073, Blast_Score=132, Evalue=9e-31,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=66.1504424778761, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1786882, Length=405, Percent_Identity=26.4197530864197, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=489, Percent_Identity=23.9263803680982, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71996771, Length=304, Percent_Identity=28.2894736842105, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356207, Length=497, Percent_Identity=27.1629778672032, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=25.8620689655172, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49488; Mature: 49357

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEA
CCCCCCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCEEEEEECCCHHHHH
VQESLDAIGEALAENGKVIVTGCLGAKKDAAGQDIITSVHPKVLAVTGPHALGEVMEAVH
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHH
THLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVM
HHCCCCCCCCEECCCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHHHH
LEAENLLKAGVKELLVISQDTSAYGVDVKFRTGFWNGRPLKTRMTELVGALGELAAQYGA
HHHHHHHHHHHHHHEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPEVLKRMKRPANAEKTLDRIRAW
EEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
REVCPELTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGAL
HHHCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCC
PDEVREERRARFMEVAERVSARRLQRKVGKTLRVLVDEVNQDGGIGRSSADAPEIDGLVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
IAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV
ECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SDVSTQSPKVGFVSLGCPKALVDSEQIITQLRAEGYEISGTYGGADLVVVNTCGFIDEA
CCCCCCCCCCEEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCEEEEEECCCHHHHH
VQESLDAIGEALAENGKVIVTGCLGAKKDAAGQDIITSVHPKVLAVTGPHALGEVMEAVH
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHHHHHH
THLPKPHDPFIDLVPPQGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVM
HHCCCCCCCCEECCCCCCCEECCCCEEEEEECCCCCCCCCEEEECCCCCHHHHCHHHHHH
LEAENLLKAGVKELLVISQDTSAYGVDVKFRTGFWNGRPLKTRMTELVGALGELAAQYGA
HHHHHHHHHHHHHHEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
WVRLHYVYPYPSVDEVMPLMAEGKVLPYLDVPLQHAHPEVLKRMKRPANAEKTLDRIRAW
EEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
REVCPELTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGAL
HHHCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCC
PDEVREERRARFMEVAERVSARRLQRKVGKTLRVLVDEVNQDGGIGRSSADAPEIDGLVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEE
IAPPSKPYKRYKAGDFVSVKITGADGHDLWGEV
ECCCCCHHHHCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA