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The map label for this gene is lpdA [H]

Identifier: 17546322

GI number: 17546322

Start: 1722543

End: 1724327

Strand: Direct

Name: lpdA [H]

Synonym: RSc1603

Alternate gene names: 17546322

Gene position: 1722543-1724327 (Clockwise)

Preceding gene: 17546321

Following gene: 17546325

Centisome position: 46.35

GC content: 67.23

Gene sequence:

>1785_bases
ATGAGCGTGGTGGAAATCAAGGTGCCGGACATCGGCGACTACAAGGACGTGGACGTCATCGAAGTGATGGTGAAGGCGGG
CGACGTCGTCGCCGTCGACCAGTCGCTGATCACGCTGGAGACCGACAAGGCGACCATGGACGTGCCGGCCGACGTGGCAG
GCAGGATCGTCGAGGTCAAGATTAAGGTGGGCGACAAGGCCAGCCAGGGCACCGTCATCGCCACGGTGGAAGCCGGTGCC
GCGGCCGCGGCGCCCGCGCCCACGCAAGCCCCGGCTCCGGCGGCCGCACCCGCTGCCGCTCCGGCCGCCTCGGCGCCCGC
GCCGCAGGCCGCGAAGCACGGCGGCAGCGCCGATCTCGAATGCGACATGCTCGTCCTGGGCTCCGGCCCGGGCGGCTACT
CGGCCGCTTTCCGCAGCGCCGACCTCGGCATGAACACGGTGCTGGTCGAACGCTTCGGCACGCTGGGCGGCGTCTGCCTG
AATGTGGGGTGCATCCCGTCCAAGGCGCTGCTGCACACGGCGGCCATCATGGACGAAGTCAAGGCGATGGCCAGCCACGG
CATCGTCTACAGCGAGCCGAAGGTCGATCTGGACCAACTGCGCAAGCACAAGGAAACCGTGGTCGGCAAGCTGACCGGCG
GCCTGGCCGGCATGGCCAAGTCGCGCAAGGTGCAAGTCGTGCGCGGCGTGGGCACGTTCCTCGACCCGAACCATCTGGAA
GTTCAGCTGACCAGCGGTGAAGGCAAGCAGGCCACCGGCGAGAAGAAGGTCATCCGCTTCGCCAAGGCCATCATCGCCGC
CGGCAGCGAGTCGGTGAAGCTGCCGTTCATTCCGGAAGACCCGCGCATCGTCGACTCCACCGGCGCGCTGGAGCTGCGCC
AGGTGCCGGGCAAGATGCTGGTCATCGGCGGCGGCATCATCGGCCTGGAAATGGCGACGGTTTACAGCACGCTGGGTGCG
CGCATCGACGTGGTGGAGATGCTCGATAGCCTGATGAACGGCGCCGACCGCGATCTCGTCAAGGTGTGGGACAAGATGAA
CAAGTCCCGCTTCGACAAGGTCATGCTCAAGACCAAGACGGTGGGTGTCGAGGCCAAGCCGGACGGCATTTACGTCAGGT
TCGAGGGCGAGCAGGCGCCCGCCGAGCCGCAGCGCTATGACATGGTGCTGGTGGCCGTGGGCCGCAGCCCGAACGGCAAG
CGCATCGGCGCGGAAAAGGCCGGCGTGGCCGTGACCGACCGCGGCTTCATCGACGTGGACAAGCAGCAGCGCACCAACGT
GCCGCATATCTATGCGATCGGCGACATCGTCGGGCAGCCGATGCTGGCGCACAAGGCGGTGCACGAGGCCCACGTCGCGG
CGGAGGCCGCGCATGGCGAGAAGGCCTACTTCGATGCCAAGCAGATTCCGTCGGTGGCCTTCACCGATCCGGAAGTGGCC
TGGGCCGGCCTGACCGAAGACCAGTGCAAGGCGCAGGGCATCAAGTACGGCAAGGGCGTGTTCCCGTGGGCCGCTTCGGG
CCGCGCCATCGCCAACGGCCGCGATGAGGGCTTCACCAAGGTCATCTTCGACGAAGAGACGCACCGGATTATCGGCGGCG
GCATCGTCGGCACGCATGCAGGCGACCTGATCAGCGAGATCTGCTTGGCGATCGAGATGGGCGCGGACGCGATCGACATC
GGCAAGACCATCCACCCGCATCCGACGCTGGGCGAGTCGGTTGGCATGGCGGCGGAAATCTACGAGGGCGTCTGCACGGA
CGTGCCGCCGGCTCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
AGGCGGCCCGATCGAGGTGCATACGGTGCGCCTTCAGGATGGATAGCGCCGGCAGCTGCAGCGAGGGCTGGGCGCCGGTG
ACAGGAACAAGGAGTCAGGA

Downstream 100 bases:

>100_bases
AATGCAATTAACCGCCAGGTTTTCGAATCGACCTTGAGCCCTGGCTGACAAAGAAAGAGCCGCTCCCATGGGCGGCTCTT
TTGATTTGGCGGCGGATCAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MSVVEIKVPDIGDYKDVDVIEVMVKAGDVVAVDQSLITLETDKATMDVPADVAGRIVEVKIKVGDKASQGTVIATVEAGA
AAAAPAPTQAPAPAAAPAAAPAASAPAPQAAKHGGSADLECDMLVLGSGPGGYSAAFRSADLGMNTVLVERFGTLGGVCL
NVGCIPSKALLHTAAIMDEVKAMASHGIVYSEPKVDLDQLRKHKETVVGKLTGGLAGMAKSRKVQVVRGVGTFLDPNHLE
VQLTSGEGKQATGEKKVIRFAKAIIAAGSESVKLPFIPEDPRIVDSTGALELRQVPGKMLVIGGGIIGLEMATVYSTLGA
RIDVVEMLDSLMNGADRDLVKVWDKMNKSRFDKVMLKTKTVGVEAKPDGIYVRFEGEQAPAEPQRYDMVLVAVGRSPNGK
RIGAEKAGVAVTDRGFIDVDKQQRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVA
WAGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKVIFDEETHRIIGGGIVGTHAGDLISEICLAIEMGADAIDI
GKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR

Sequences:

>Translated_594_residues
MSVVEIKVPDIGDYKDVDVIEVMVKAGDVVAVDQSLITLETDKATMDVPADVAGRIVEVKIKVGDKASQGTVIATVEAGA
AAAAPAPTQAPAPAAAPAAAPAASAPAPQAAKHGGSADLECDMLVLGSGPGGYSAAFRSADLGMNTVLVERFGTLGGVCL
NVGCIPSKALLHTAAIMDEVKAMASHGIVYSEPKVDLDQLRKHKETVVGKLTGGLAGMAKSRKVQVVRGVGTFLDPNHLE
VQLTSGEGKQATGEKKVIRFAKAIIAAGSESVKLPFIPEDPRIVDSTGALELRQVPGKMLVIGGGIIGLEMATVYSTLGA
RIDVVEMLDSLMNGADRDLVKVWDKMNKSRFDKVMLKTKTVGVEAKPDGIYVRFEGEQAPAEPQRYDMVLVAVGRSPNGK
RIGAEKAGVAVTDRGFIDVDKQQRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVA
WAGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKVIFDEETHRIIGGGIVGTHAGDLISEICLAIEMGADAIDI
GKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR
>Mature_593_residues
SVVEIKVPDIGDYKDVDVIEVMVKAGDVVAVDQSLITLETDKATMDVPADVAGRIVEVKIKVGDKASQGTVIATVEAGAA
AAAPAPTQAPAPAAAPAAAPAASAPAPQAAKHGGSADLECDMLVLGSGPGGYSAAFRSADLGMNTVLVERFGTLGGVCLN
VGCIPSKALLHTAAIMDEVKAMASHGIVYSEPKVDLDQLRKHKETVVGKLTGGLAGMAKSRKVQVVRGVGTFLDPNHLEV
QLTSGEGKQATGEKKVIRFAKAIIAAGSESVKLPFIPEDPRIVDSTGALELRQVPGKMLVIGGGIIGLEMATVYSTLGAR
IDVVEMLDSLMNGADRDLVKVWDKMNKSRFDKVMLKTKTVGVEAKPDGIYVRFEGEQAPAEPQRYDMVLVAVGRSPNGKR
IGAEKAGVAVTDRGFIDVDKQQRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVAW
AGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKVIFDEETHRIIGGGIVGTHAGDLISEICLAIEMGADAIDIG
KTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=42.5162689804772, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI50301238, Length=477, Percent_Identity=26.8343815513627, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI148277071, Length=449, Percent_Identity=27.3942093541203, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI148277065, Length=449, Percent_Identity=27.3942093541203, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI33519430, Length=449, Percent_Identity=27.3942093541203, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI33519428, Length=449, Percent_Identity=27.3942093541203, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI33519426, Length=449, Percent_Identity=27.3942093541203, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=28.1179138321995, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI22035672, Length=438, Percent_Identity=28.310502283105, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI291045268, Length=434, Percent_Identity=26.7281105990783, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1786307, Length=480, Percent_Identity=63.5416666666667, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=27.7542372881356, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=25.9825327510917, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=27.1493212669683, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786305, Length=75, Percent_Identity=56, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32565766, Length=480, Percent_Identity=39.375, Blast_Score=322, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=28.8702928870293, Blast_Score=139, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=25.2676659528908, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=25.2676659528908, Blast_Score=119, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=452, Percent_Identity=25.6637168141593, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=459, Percent_Identity=40.3050108932462, Blast_Score=304, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=27.6729559748428, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=25.7383966244726, Blast_Score=136, Evalue=9e-33,
Organism=Drosophila melanogaster, GI21358499, Length=464, Percent_Identity=41.1637931034483, Blast_Score=335, Evalue=6e-92,
Organism=Drosophila melanogaster, GI24640551, Length=542, Percent_Identity=28.9667896678967, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=29.0650406504065, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=29.253112033195, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=26.530612244898, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 62188; Mature: 62057

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVEIKVPDIGDYKDVDVIEVMVKAGDVVAVDQSLITLETDKATMDVPADVAGRIVEVK
CCEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCHHHCCEEEEEE
IKVGDKASQGTVIATVEAGAAAAAPAPTQAPAPAAAPAAAPAASAPAPQAAKHGGSADLE
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCE
CDMLVLGSGPGGYSAAFRSADLGMNTVLVERFGTLGGVCLNVGCIPSKALLHTAAIMDEV
EEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
KAMASHGIVYSEPKVDLDQLRKHKETVVGKLTGGLAGMAKSRKVQVVRGVGTFLDPNHLE
HHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCCEE
VQLTSGEGKQATGEKKVIRFAKAIIAAGSESVKLPFIPEDPRIVDSTGALELRQVPGKML
EEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEECCCCCEEHCCCCCEEE
VIGGGIIGLEMATVYSTLGARIDVVEMLDSLMNGADRDLVKVWDKMNKSRFDKVMLKTKT
EEECCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHEEEEEE
VGVEAKPDGIYVRFEGEQAPAEPQRYDMVLVAVGRSPNGKRIGAEKAGVAVTDRGFIDVD
ECEEECCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEECC
KQQRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVA
HHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCHHEECHHHCCCEEECCCCCE
WAGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKVIFDEETHRIIGGGIVGTHA
EECCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCEECCCH
GDLISEICLAIEMGADAIDIGKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVVEIKVPDIGDYKDVDVIEVMVKAGDVVAVDQSLITLETDKATMDVPADVAGRIVEVK
CEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCHHHCCEEEEEE
IKVGDKASQGTVIATVEAGAAAAAPAPTQAPAPAAAPAAAPAASAPAPQAAKHGGSADLE
EEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCE
CDMLVLGSGPGGYSAAFRSADLGMNTVLVERFGTLGGVCLNVGCIPSKALLHTAAIMDEV
EEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
KAMASHGIVYSEPKVDLDQLRKHKETVVGKLTGGLAGMAKSRKVQVVRGVGTFLDPNHLE
HHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCCEE
VQLTSGEGKQATGEKKVIRFAKAIIAAGSESVKLPFIPEDPRIVDSTGALELRQVPGKML
EEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEECCCCCEEHCCCCCEEE
VIGGGIIGLEMATVYSTLGARIDVVEMLDSLMNGADRDLVKVWDKMNKSRFDKVMLKTKT
EEECCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHEEEEEE
VGVEAKPDGIYVRFEGEQAPAEPQRYDMVLVAVGRSPNGKRIGAEKAGVAVTDRGFIDVD
ECEEECCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEECC
KQQRTNVPHIYAIGDIVGQPMLAHKAVHEAHVAAEAAHGEKAYFDAKQIPSVAFTDPEVA
HHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCCCHHEECHHHCCCEEECCCCCE
WAGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKVIFDEETHRIIGGGIVGTHA
EECCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCEECCCH
GDLISEICLAIEMGADAIDIGKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]