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The map label for this gene is hpaI1 [H]

Identifier: 17546310

GI number: 17546310

Start: 1706911

End: 1707729

Strand: Direct

Name: hpaI1 [H]

Synonym: RSc1591

Alternate gene names: 17546310

Gene position: 1706911-1707729 (Clockwise)

Preceding gene: 17546309

Following gene: 17546313

Centisome position: 45.93

GC content: 71.31

Gene sequence:

>819_bases
ATGCAGCTTCCGGTGAACCCTTTTAAGCGCGCGCTGGCCGAACGACGCCCGCAGATCGGCCTGTGGCTGGCGATGGCGCA
TCCATACCCCGCCGAGATTGTTGCCGGGGCCGGCTTCGACTGGCTGCTGATCGACGGCGAGCACGCGCCCAACCAGCTGC
AGTCGACGCTGGCGCAACTGCAGGCGGTGGCGCCGTATCCCGCGCATCCGATCGTGCGGCCGGCGTGGAACGACGTGGTG
GAGATCAAGCGCCTGCTCGACATCGGCGCACAGACGTTGCTGATCCCGATGGTGCAGAGCGCGGAGGAGGCGCGCGCGGC
CGTGGCGACGATGCGCTATCCGCCCCACGGTAACCGGGGCGTGGGCAGCGCACTGGCGCGGGCATCGCGCTGGAACCGCG
TGGACGACTACGTCCGCCGCGCCGACGCCGAGATGTGCACGCTGGTGCAGGTGGAAACGCCGCGCGGCGTCGAGCATCTG
GACGCCATCCTGGCTGTGGCGGGTGTCGACGGTGTCTTCATCGGGCCGGGCGACCTGGCTGCGTCGATGGGGCACCTGGG
CGAGCCGGGCCATCCGGAGGTGCGCCAGACCATCGATCGCACCATCCGGCGCATCGTTGCGGCGGGCAAGGCGGCGGGTA
TCTTGTCGGCCGATCCGGCGCTGGCGCGGCACTACCTGTCGCTGGGGGCGACCTTTGTTGCCGTGGGGTCGGATGTCACG
CTGCTGGCCCGCAGCGCCGAGCAGTTGGCGCGGTCGTTCGCTGGCGCTGGCGCTGGCGCGGGCGCACCCGTGCCGGGGCA
GGCCGGCGCGGTGTACTGA

Upstream 100 bases:

>100_bases
GGTGAGACCTGCCGGTGACATGGTGTCCCCGGGGCAGCATCGGCTGCCACGTCGTCCCGCTTGTCCAACTGTTTTACATT
CCGTGGAGGAGCCGTCCGCG

Downstream 100 bases:

>100_bases
CGTCTCGGTATCGCGGGCGGGCGGTCAGTCGTCGGCCGGGAGGCGTTTGTCCGTGGCGGGCGCTTCCATCAACTGGATGG
CCTCGTCGATGACGCGGTAG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase oxidoreductase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MQLPVNPFKRALAERRPQIGLWLAMAHPYPAEIVAGAGFDWLLIDGEHAPNQLQSTLAQLQAVAPYPAHPIVRPAWNDVV
EIKRLLDIGAQTLLIPMVQSAEEARAAVATMRYPPHGNRGVGSALARASRWNRVDDYVRRADAEMCTLVQVETPRGVEHL
DAILAVAGVDGVFIGPGDLAASMGHLGEPGHPEVRQTIDRTIRRIVAAGKAAGILSADPALARHYLSLGATFVAVGSDVT
LLARSAEQLARSFAGAGAGAGAPVPGQAGAVY

Sequences:

>Translated_272_residues
MQLPVNPFKRALAERRPQIGLWLAMAHPYPAEIVAGAGFDWLLIDGEHAPNQLQSTLAQLQAVAPYPAHPIVRPAWNDVV
EIKRLLDIGAQTLLIPMVQSAEEARAAVATMRYPPHGNRGVGSALARASRWNRVDDYVRRADAEMCTLVQVETPRGVEHL
DAILAVAGVDGVFIGPGDLAASMGHLGEPGHPEVRQTIDRTIRRIVAAGKAAGILSADPALARHYLSLGATFVAVGSDVT
LLARSAEQLARSFAGAGAGAGAPVPGQAGAVY
>Mature_272_residues
MQLPVNPFKRALAERRPQIGLWLAMAHPYPAEIVAGAGFDWLLIDGEHAPNQLQSTLAQLQAVAPYPAHPIVRPAWNDVV
EIKRLLDIGAQTLLIPMVQSAEEARAAVATMRYPPHGNRGVGSALARASRWNRVDDYVRRADAEMCTLVQVETPRGVEHL
DAILAVAGVDGVFIGPGDLAASMGHLGEPGHPEVRQTIDRTIRRIVAAGKAAGILSADPALARHYLSLGATFVAVGSDVT
LLARSAEQLARSFAGAGAGAGAPVPGQAGAVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=254, Percent_Identity=52.755905511811, Blast_Score=275, Evalue=2e-75,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=48.3870967741936, Blast_Score=237, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28694; Mature: 28694

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPVNPFKRALAERRPQIGLWLAMAHPYPAEIVAGAGFDWLLIDGEHAPNQLQSTLAQL
CCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
QAVAPYPAHPIVRPAWNDVVEIKRLLDIGAQTLLIPMVQSAEEARAAVATMRYPPHGNRG
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VGSALARASRWNRVDDYVRRADAEMCTLVQVETPRGVEHLDAILAVAGVDGVFIGPGDLA
HHHHHHHHHHCHHHHHHHHHHCHHHEEEEEEECCCCHHHHHHHHHHHCCCEEEECCHHHH
ASMGHLGEPGHPEVRQTIDRTIRRIVAAGKAAGILSADPALARHYLSLGATFVAVGSDVT
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHEEEECCCHH
LLARSAEQLARSFAGAGAGAGAPVPGQAGAVY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLPVNPFKRALAERRPQIGLWLAMAHPYPAEIVAGAGFDWLLIDGEHAPNQLQSTLAQL
CCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
QAVAPYPAHPIVRPAWNDVVEIKRLLDIGAQTLLIPMVQSAEEARAAVATMRYPPHGNRG
HHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VGSALARASRWNRVDDYVRRADAEMCTLVQVETPRGVEHLDAILAVAGVDGVFIGPGDLA
HHHHHHHHHHCHHHHHHHHHHCHHHEEEEEEECCCCHHHHHHHHHHHCCCEEEECCHHHH
ASMGHLGEPGHPEVRQTIDRTIRRIVAAGKAAGILSADPALARHYLSLGATFVAVGSDVT
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHEEEECCCHH
LLARSAEQLARSFAGAGAGAGAPVPGQAGAVY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA