Click here to switch to the map view.

The map label for this gene is pheS [H]

Identifier: 17546300

GI number: 17546300

Start: 1695887

End: 1696921

Strand: Direct

Name: pheS [H]

Synonym: RSc1581

Alternate gene names: 17546300

Gene position: 1695887-1696921 (Clockwise)

Preceding gene: 17546299

Following gene: 17546301

Centisome position: 45.63

GC content: 64.15

Gene sequence:

>1035_bases
ATGTCACTGGATCTGGACCAAGTCGTCGCCGATGCCCAAACTGCGTTTGCCTCTGTGGAGGACAACGCTTCGCTGGAAAA
CGAGAAGGCGCGCTTTCTCGGCAAGTCCGGCGTGCTGACCGACTTGCTCAAGGGACTGGGCAAGCTCGATCCGCAGACGC
GCAAGTCGGAAGGCGCGCGTATCAACCTGGCCAAGAGCCGCGTCGAAGCGGCCCTGAACGACCGCCGCCAGGCGCTGGCT
GACGCGCTGATGAACGCGCGCCTGGCCGCCGAGGCGATCGACGTGACGTTGCCCGGCCGCGATGTGGCCGAGGGCAGCCT
GCACCCCGTGATGAACACCTGGGAGCGCGTCGCCCAGATCTTCGGCTCGATCGGCTTCGACGTGGCCGATGGCCCCGAGA
TCGAAACCGACTGGATGAACTTCACGGCGCTGAACAACCCGGACAACCATCCGGCGCGCTCGATGCAGGACACCTTCTAC
ATCGACGGCCGCGACAGCGACGACAAGCTCCTGCTGCTGCGCACGCATACCAGCCCGATGCAGGTGCGCTATGCCCGCAT
GCATGTCGAGAAATACAAGCACCTCGATCGCATCCCGCCGATCAAGGTGATCGCGCCGGGCCGCACCTACCGCGTCGACA
GCGATGCCACGCACTCGCCGATGTTCCATCAGGTCGAAGGCCTCTGGATCGCGGACAACATCAGCTTCGCGGATCTCAAG
GGCGTCTACACCGACTTCCTGCGCAAGTTCTTCGAGAGCGACGATATTCAGGTGCGCTTCCGCCCGTCGTACTTCCCGTT
CACCGAACCGTCGGCCGAGATCGACATGGCCTTCGGCAACGGCAGGTGGCTGGAGATTTCCGGTTCCGGCCAGGTGCACC
CGACCGTGGTGCGCAACATGGGCCTCGATCCGGAGCGCTATATCGGCTTTGCGTTCGGCTCGGGCCTGGAGCGCCTGACC
ATGCTGCGCTACGGCATCAACGACCTGCGCCTGTTCTTCGAGGGCGACGTGCGCTTCCTGCGCCAGTTCGCGTAA

Upstream 100 bases:

>100_bases
GTCAGTCCGTTCCGCGGTTTGTCGGCCGATCACCCTGACGGGGCTCTCACCGAGCCCCGTTTTTTATTCCGAATTCCCGA
ATTCTCACGCTCGCCTTCCC

Downstream 100 bases:

>100_bases
CGCGGACGCCTGCCGTCCGCGGGTTCGCCGTTTCCCTGTTCACCGAATTCCTTTTTCTGTCGCGCCATGCAATTTCCGGA
ATCCTGGCTTCGCAGTTTCG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSLDLDQVVADAQTAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPQTRKSEGARINLAKSRVEAALNDRRQALA
DALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFY
IDGRDSDDKLLLLRTHTSPMQVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
GVYTDFLRKFFESDDIQVRFRPSYFPFTEPSAEIDMAFGNGRWLEISGSGQVHPTVVRNMGLDPERYIGFAFGSGLERLT
MLRYGINDLRLFFEGDVRFLRQFA

Sequences:

>Translated_344_residues
MSLDLDQVVADAQTAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPQTRKSEGARINLAKSRVEAALNDRRQALA
DALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFY
IDGRDSDDKLLLLRTHTSPMQVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
GVYTDFLRKFFESDDIQVRFRPSYFPFTEPSAEIDMAFGNGRWLEISGSGQVHPTVVRNMGLDPERYIGFAFGSGLERLT
MLRYGINDLRLFFEGDVRFLRQFA
>Mature_343_residues
SLDLDQVVADAQTAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPQTRKSEGARINLAKSRVEAALNDRRQALAD
ALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYI
DGRDSDDKLLLLRTHTSPMQVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLKG
VYTDFLRKFFESDDIQVRFRPSYFPFTEPSAEIDMAFGNGRWLEISGSGQVHPTVVRNMGLDPERYIGFAFGSGLERLTM
LRYGINDLRLFFEGDVRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=33.7078651685393, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI5729820, Length=295, Percent_Identity=30.8474576271186, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1788007, Length=339, Percent_Identity=54.8672566371681, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=33.8289962825279, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=33.8289962825279, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=254, Percent_Identity=31.8897637795276, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=371, Percent_Identity=28.8409703504043, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=43.3333333333333, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18858079, Length=290, Percent_Identity=31.0344827586207, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17137424, Length=295, Percent_Identity=27.4576271186441, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38751; Mature: 38620

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDLDQVVADAQTAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPQTRKSEGAR
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHCCCCCE
INLAKSRVEAALNDRRQALADALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAQI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYIDGRDSDDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
HHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHCCEEEECCCCHHHHH
GVYTDFLRKFFESDDIQVRFRPSYFPFTEPSAEIDMAFGNGRWLEISGSGQVHPTVVRNM
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHC
GLDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEECCCHHHHHHCC
>Mature Secondary Structure 
SLDLDQVVADAQTAFASVEDNASLENEKARFLGKSGVLTDLLKGLGKLDPQTRKSEGAR
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHCCCCCE
INLAKSRVEAALNDRRQALADALMNARLAAEAIDVTLPGRDVAEGSLHPVMNTWERVAQI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
FGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYIDGRDSDDKLLLLRTHTSPM
HHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCH
QVRYARMHVEKYKHLDRIPPIKVIAPGRTYRVDSDATHSPMFHQVEGLWIADNISFADLK
HHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHCCEEEECCCCHHHHH
GVYTDFLRKFFESDDIQVRFRPSYFPFTEPSAEIDMAFGNGRWLEISGSGQVHPTVVRNM
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHC
GLDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA
CCCHHHHEEEHHHCHHHHHHHHHHCCCCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA