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The map label for this gene is thrS

Identifier: 17546296

GI number: 17546296

Start: 1692527

End: 1694434

Strand: Direct

Name: thrS

Synonym: RSc1577

Alternate gene names: 17546296

Gene position: 1692527-1694434 (Clockwise)

Preceding gene: 17546295

Following gene: 17546297

Centisome position: 45.54

GC content: 63.21

Gene sequence:

>1908_bases
ATGATCGCGATTACGTTGCCGGACGGTTCCCGGCGCGAGTTTCCCGGCCCGGTGACGGTGGCCGAAGTGGCGCAGAGCAT
CGGCGCGGGCTTGGCCAAGGCCGCGCTGGCCGGCCGCATTGACGGGCAACTGGTCGACACGAGCTTCAGGATCGAGCAGA
ACGTCGACCTGGCCATCGTCACGGACAAGGATACGGACGGCCTCGACGTGATTCGCCACTCGACGGCGCACTTGCTGGCC
TATGCCGTCAAGGAGCTGTACCCGGAAGCCCAGGTCACGATCGGCCCGGTCATCGAAAACGGGTTCTACTACGACTTCGC
CTACAAGCGTCCCTTTACGCCGGAAGACCTCGTCGCCATCGAGAAACGGATGGCCGAACTGGCCAAAAAGGACGAAAAGG
TCACGCGCGAAGTCTGGAGCCGCGACGACGCCGTCAAGCTGTTCGAGGGCATGGGCGAGAAGTACAAGGCGGAGATCATC
GCCTCGATTCCGGAAGACCAGGAAATCGGCCTGTACCGCGAAGGCAATTTCGTCGACCTGTGCCGTGGCCCGCACGTGCC
GTCCACGGGCAAGCTCAAGGTCTTCAAGCTGATGAAGGTGGCCGGCGCCTACTGGCGCGGCGACTCCAACAACGAGATGC
TCCAGCGCATCTACGGCACGGCCTGGGCCAAGAAGGAAGACCAGGAGGCCTACCTGCACATGCTGGAAGAGGCCGAGAAG
CGCGACCACCGCAAGCTCGGCAAGTTGCTCGACCTGTTCCACCTGCAGGAAGAAGCGCCCGGCATGGTGTTCTGGCACCC
GAAGGGCTGGCAGATCTGGCAGGCCGTCGAGCAATACATGCGCGGCCGCCTGGCCGGCGCCGGCTACAGCGAAGTCCGCA
CGCCGCAAGTGATGGATCGCGGCCTGTGGGAGCGCTCCGGCCACTGGGAGAACTACAAAGAGAACATGTTCGTGACGGAA
TCGGAAAAGCGCGAATACGCGATCAAGCCGATGAACTGTCCCGGCCATGTGCAGATTTTCAACCACGGCCTGCGCTCGTA
TCGCGACCTGCCGCTGCGCCTGGCCGAGTTCGGCGCGTGCCATCGCAATGAGCCGTCCGGCGCGCTGCATGGCCTGATGC
GCGTGCGCGGGTTTGTGCAGGATGATGCGCACATCTTCTGCACGGAAGGCCAGATCATGGCCGAGGCCAAGGACTTCAAC
GACCTGGCCTTCAGCATCTACGAGGACTTCGGCTTCGAGAACGTCGCCGTCAAGCTGGCGCTGCGTCCGGACAAGCGGGC
CGGCACGGACGAAATCTGGGATCACGCCGAGGAAGGCCTGCGCACGGCGCTGCGCGCCTGCGGCGTCGAATGGGAAGAGT
TGCCGGGCGAGGGCGCTTTCTACGGCCCGAAGGTCGAGTACCACATCAAGGACGCCATCGGCCGTTCGTGGCAGTGCGGC
ACGCTGCAGCTCGACCTGGTGCTGCCCGAGCGACTGGGTGCCGAGTATGTCGCGGAAGACAACAGCCGCAAGCGCCCGGT
CATGCTCCACCGCGCCATCCTGGGCTCGTTCGAGCGCTTCCTGGGCATTTTGCTGGAAAACCATGCCGGCGCGCTGCCGC
TGTGGCTGGCGCCGGAGCAGGTGGTGATCATGAGCATCGCCGATGCCCACGCCGAATATGCGGAATCCGTTGCGCAATCC
CTGCAAAAACAAGGGTTTAGGGCGTCCGCCGATTTGCGGAACGAGAAAATTACGTATAAAATCCGCGAGCACTCCTTGCA
GAAGGTCCCCTACCTCGTTGTGGTGGGCGACAAGGAGCGCGACGGAAATCAGGTGGCCGTGCGTGCCCGTGGCAACGTCG
ATCTCGGTTCGATGCCGGTATCCACGTTCGTTGAGCGACTGCAGAACGATTTCGCCAACAAGTCATGA

Upstream 100 bases:

>100_bases
CCGGCCCGACAGACTGCCGCACCTGCCTGGTGCGGCGCACGACAGAATGATAACCGCCGCGTATACGCACCCGCGCGACG
GAGCCTCCACAGGAGCAAGC

Downstream 100 bases:

>100_bases
GCTGGGCACGGCTTGTTTTTTGCTTCTTTGAGGACATTCAACATCGCTACTGAGAAATCTCACCGCATCAACCGCGAGAT
CACCGCGCCGGAAATCCGGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIVTDKDTDGLDVIRHSTAHLLA
YAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEII
ASIPEDQEIGLYREGNFVDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEK
RDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDRGLWERSGHWENYKENMFVTE
SEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGACHRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFN
DLAFSIYEDFGFENVAVKLALRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCG
TLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQVVIMSIADAHAEYAESVAQS
LQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKERDGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS

Sequences:

>Translated_635_residues
MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIVTDKDTDGLDVIRHSTAHLLA
YAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEII
ASIPEDQEIGLYREGNFVDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEK
RDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDRGLWERSGHWENYKENMFVTE
SEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGACHRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFN
DLAFSIYEDFGFENVAVKLALRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCG
TLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQVVIMSIADAHAEYAESVAQS
LQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKERDGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS
>Mature_635_residues
MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIVTDKDTDGLDVIRHSTAHLLA
YAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAIEKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEII
ASIPEDQEIGLYREGNFVDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEK
RDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDRGLWERSGHWENYKENMFVTE
SEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGACHRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFN
DLAFSIYEDFGFENVAVKLALRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCG
TLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQVVIMSIADAHAEYAESVAQS
LQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKERDGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=631, Percent_Identity=40.2535657686212, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI38570111, Length=615, Percent_Identity=40.3252032520325, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI20070344, Length=647, Percent_Identity=37.7125193199382, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=58.3333333333333, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=163, Percent_Identity=31.2883435582822, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71984184, Length=631, Percent_Identity=39.1442155309033, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=39.1442155309033, Blast_Score=474, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322112, Length=652, Percent_Identity=39.5705521472393, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322654, Length=446, Percent_Identity=36.322869955157, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=40.3174603174603, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=40.3174603174603, Blast_Score=495, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=40.3174603174603, Blast_Score=495, Evalue=1e-140,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_RALSO (Q8XZ29)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519698.1
- ProteinModelPortal:   Q8XZ29
- SMR:   Q8XZ29
- GeneID:   1220409
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1577
- NMPDR:   fig|267608.1.peg.1577
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- ProtClustDB:   PRK00413
- BioCyc:   RSOL267608:RSC1577-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 71812; Mature: 71812

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIV
CEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEECCEEEEECCCCEEEE
TDKDTDGLDVIRHSTAHLLAYAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCHHHHHHH
EKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEIIASIPEDQEIGLYREGNFVDL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHEECCCCCCCCEEECCCEEEE
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDR
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHC
GLWERSGHWENYKENMFVTESEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGAC
CHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFNDLAFSIYEDFGFENVAVKLA
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCHHHHHHHHHHCCCCEEEEEEE
LRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCG
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEHHHCCCCCCCC
TLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQ
EEEEEEECHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCE
VVIMSIADAHAEYAESVAQSLQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKER
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHCCEEEEECCCCC
DGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIV
CEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCEEECCEEEEECCCCEEEE
TDKDTDGLDVIRHSTAHLLAYAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCHHHHHHH
EKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEIIASIPEDQEIGLYREGNFVDL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHEECCCCCCCCEEECCCEEEE
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDR
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHC
GLWERSGHWENYKENMFVTESEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGAC
CHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCC
HRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFNDLAFSIYEDFGFENVAVKLA
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCHHHHHHHHHHCCCCEEEEEEE
LRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCG
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEHHHCCCCCCCC
TLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQ
EEEEEEECHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCE
VVIMSIADAHAEYAESVAQSLQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKER
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHCCEEEEECCCCC
DGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852