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The map label for this gene is thiED [H]

Identifier: 17230261

GI number: 17230261

Start: 3364727

End: 3365347

Strand: Direct

Name: thiED [H]

Synonym: alr2769

Alternate gene names: 17230261

Gene position: 3364727-3365347 (Clockwise)

Preceding gene: 17230260

Following gene: 17230263

Centisome position: 52.46

GC content: 42.51

Gene sequence:

>621_bases
TTGACTTTCTCAAATCAACTTTGGACAGCAAATCAAGATTTAGCCGCAGCTTGCCTAGAGCATCCTTTTGTTCAAGGTAT
TGGTAATGGTAGCCTGGAGGAGCAAAAATTTGCTTATTATGTAGGGCAGGATGCTTTTTTCTTAGCAGCCTTCGCCCGTG
CTTACAGTATTGCTGCGGCTAAATCACCAGACTGGATAGGTTTTACTACGTTTCACAATTTAGCTGGTGGTGTATTGGCA
GAGATGCGTCTACATGAAAGCTACGCAGTCCAATGGGGAGTTGATTTACATTCTGTGCAACCAGGAGTGGCTACCCGTCG
CTATACCGATTTTTTGTTGGCTACTGCTTGGGGTGGAGATGTAGGTTTAACTGCTGCGGCCATGTCTCCTTGTATGCGTC
TGTATGCTTTTTTGGGAGAACAGTTAGCTAAGAATGGTATTCCTAATCATCAATACGCTGATTGGATTCGGACTTACTGT
AGTGCTGATTTTCTACCATTAGTACAACAATTGGAAAGTTTAGTAGAAAACTATGCTACTGCTAATACCTTAACCAGTTC
AACTTATCGCTACGCCATGCTTTGCGAACAAGAATTTTTTCAAGCAGCCTGGATGATTTAA

Upstream 100 bases:

>100_bases
CGGTAAACCCAGTTTTTTCGACTTTTTAGGCAGTCTAGGTTCAGTATTGCTACAGTTCCTACCATTTTGAATGCTTAATT
TTGAATTGGAGGTAAGCGAC

Downstream 100 bases:

>100_bases
TCACAGCACATTCTATAGAACTCATATTTGATTTTTGAATAAAACTCAGTACACATTTATTGCTTTCTTCCCAATCCCCA
TTACCCCTTATCCCCAGAGG

Product: transcriptional regulator

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTFSNQLWTANQDLAAACLEHPFVQGIGNGSLEEQKFAYYVGQDAFFLAAFARAYSIAAAKSPDWIGFTTFHNLAGGVLA
EMRLHESYAVQWGVDLHSVQPGVATRRYTDFLLATAWGGDVGLTAAAMSPCMRLYAFLGEQLAKNGIPNHQYADWIRTYC
SADFLPLVQQLESLVENYATANTLTSSTYRYAMLCEQEFFQAAWMI

Sequences:

>Translated_206_residues
MTFSNQLWTANQDLAAACLEHPFVQGIGNGSLEEQKFAYYVGQDAFFLAAFARAYSIAAAKSPDWIGFTTFHNLAGGVLA
EMRLHESYAVQWGVDLHSVQPGVATRRYTDFLLATAWGGDVGLTAAAMSPCMRLYAFLGEQLAKNGIPNHQYADWIRTYC
SADFLPLVQQLESLVENYATANTLTSSTYRYAMLCEQEFFQAAWMI
>Mature_205_residues
TFSNQLWTANQDLAAACLEHPFVQGIGNGSLEEQKFAYYVGQDAFFLAAFARAYSIAAAKSPDWIGFTTFHNLAGGVLAE
MRLHESYAVQWGVDLHSVQPGVATRRYTDFLLATAWGGDVGLTAAAMSPCMRLYAFLGEQLAKNGIPNHQYADWIRTYCS
ADFLPLVQQLESLVENYATANTLTSSTYRYAMLCEQEFFQAAWMI

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=194, Percent_Identity=28.3505154639175, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324997, Length=196, Percent_Identity=28.0612244897959, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 22901; Mature: 22770

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSNQLWTANQDLAAACLEHPFVQGIGNGSLEEQKFAYYVGQDAFFLAAFARAYSIAAA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHC
KSPDWIGFTTFHNLAGGVLAEMRLHESYAVQWGVDLHSVQPGVATRRYTDFLLATAWGGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCCHHHHHHHHHHHHCCCCCC
VGLTAAAMSPCMRLYAFLGEQLAKNGIPNHQYADWIRTYCSADFLPLVQQLESLVENYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ANTLTSSTYRYAMLCEQEFFQAAWMI
HHHHHHCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFSNQLWTANQDLAAACLEHPFVQGIGNGSLEEQKFAYYVGQDAFFLAAFARAYSIAAA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHC
KSPDWIGFTTFHNLAGGVLAEMRLHESYAVQWGVDLHSVQPGVATRRYTDFLLATAWGGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCHHCCCCCHHHHHHHHHHHHCCCCCC
VGLTAAAMSPCMRLYAFLGEQLAKNGIPNHQYADWIRTYCSADFLPLVQQLESLVENYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ANTLTSSTYRYAMLCEQEFFQAAWMI
HHHHHHCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]