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The map label for this gene is nadE [H]

Identifier: 17229977

GI number: 17229977

Start: 2985297

End: 2986997

Strand: Direct

Name: nadE [H]

Synonym: alr2485

Alternate gene names: 17229977

Gene position: 2985297-2986997 (Clockwise)

Preceding gene: 17229976

Following gene: 17229978

Centisome position: 46.55

GC content: 42.21

Gene sequence:

>1701_bases
ATGAAAATAGCGATCGCTCAAATTAATCCTATTATTGGTGATTTAACTGGTAATGCCCAAAAAATTCTGGAAATAGCACA
GCAGGCAATAAAAAAAGGCGCAAGATTGTTGCTAACTCCAGAACTATCTTTGTGTGGCTATCCCCCACGGGATTTATTAT
TAAATCCCAGTTTTGTAGAGGCCATGAGTGTTACTTTACAGCAATTAGCTAGAGATTTGCCTGTAAATTTAGCTGTGTTA
GTAGGGACAGTTGAACCAAATCATCAAGCCCATACTACTGGGGGAAAACCTTTATTTAATAGCACAGCTTTATTAGAAAA
TGGCAAAGTCAAACAAATGTTTCATAAGCGACTATTGCCTACCTATGATGTATTTGATGAGCATCGTTATTTTGAAGCTG
GACAACAAGCGAATTATTTTGCCTTAGATAATATTAATATTGGTGTGACAATTTGCGAAGATTTATGGAATGATGAGGAG
TTTTGGGGCAAACGCAGTTACACAGCTAATCCCATTTCCGACTTAGCAATTTTAGGTGTAGATTTAATCGTTAATTTATC
GGCTTCGCCCTACAGTTTGGGTAAGCAAAGCTTTAGAGAAGCAATGCTCAGACATAGTGCATTGAGGTTTCAACAACCCG
TAATTTACACTAACCAAGTCGGCGGTAACGATGATTTAATTTTTGATGGTCGGAGTTTTGCCTTAAATCGTCAAGGCGAA
GTGATGTGTCGGGCTAAGGGTTTTGCATCTGATTTAATTACAGTAGATTTTGATGAATCACAACGAGATTTACAACTAAA
TTCTGTTGCTCCTGTTTATGAATCAGAAGATGAGGAAATTTGGCACGCTTTAGTTTTGGGTGTGCGAGATTATGCTCAAA
AATGCCGTTTTTCTCAAGTCGTGCTTGGTTTAAGCGGTGGGATTGATTCTGCACTGGTGGCGACAATTGCCACTGCGGCC
TTAGGTAAGGAAAATGTCTTTGGTGTTTTAATGCCATCTCCTTACAGTTCGGAACATTCTATTAGCGACGCTTTAGCATT
AGCCGACAACTTGGGAATTAAGACCCAAATCTTACCCATAGGGGAGTTAATGCAGAGCTTTGACCAGAGTTTAGTAGAGT
TATTTGCCGGCACAGAATTTGGTTTAGCTGAGGAGAACATCCAGTCACGGATTCGCGGTAACTTATTAATGGCGATCGCT
AATAAATTTGGCTACCTGTTATTATCCACCGGTAATAAGTCGGAAATGGCAGTCGGTTACTGCACCCTCTACGGCGACAT
GAACGGCGGTTTAGCAGTCATCGCCGACGTTCCCAAAACCCGCGTTTACTCCCTTTGTAACTGGCTAAATTCCCACCAAT
CCCCAATCATCCCCGAAAACATCCTTACTAAAGCACCAAGCGCCGAACTCAAACCCGGTCAAGTTGACCAAGATTCCCTA
CCCCCATACGAAATTTTGGACGACATATTACAGCGCTTCATTCACAACCATCAATCCGTAGGGGAAATTGTCGCCGCAGG
TCATGAACCTGTAGTGGTAGACAGAGTTATCCAGATGGTTGCACGAGCTGAATTTAAGCGCCGCCAAGCACCCCCAGGAC
TAAAAATCACCGACCGCGCTTTTGGCACTGGTTGGCGAATGCCAATTGCTAGCAACTGGAATGCGATTAAAAACAATTAC
CGCCTAAGTTACACATTCTGA

Upstream 100 bases:

>100_bases
GACAAACCTTTGGTATTTATCTAATTGGTAATTATTGCCTAAATTATTGTAGAGAGGTTGCAATGCAACCTCTCTACCAA
AAACCTATTAATTAAAAACA

Downstream 100 bases:

>100_bases
AAGCCTGATTCAATGAATGGTAGTGTAGATGAGAAAATGGTGTAACCAATTTACTCAATGATGGCTGCTGTTCTTCTCGA
AAACGTCTATAAATTTTATA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKIAIAQINPIIGDLTGNAQKILEIAQQAIKKGARLLLTPELSLCGYPPRDLLLNPSFVEAMSVTLQQLARDLPVNLAVL
VGTVEPNHQAHTTGGKPLFNSTALLENGKVKQMFHKRLLPTYDVFDEHRYFEAGQQANYFALDNINIGVTICEDLWNDEE
FWGKRSYTANPISDLAILGVDLIVNLSASPYSLGKQSFREAMLRHSALRFQQPVIYTNQVGGNDDLIFDGRSFALNRQGE
VMCRAKGFASDLITVDFDESQRDLQLNSVAPVYESEDEEIWHALVLGVRDYAQKCRFSQVVLGLSGGIDSALVATIATAA
LGKENVFGVLMPSPYSSEHSISDALALADNLGIKTQILPIGELMQSFDQSLVELFAGTEFGLAEENIQSRIRGNLLMAIA
NKFGYLLLSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKTRVYSLCNWLNSHQSPIIPENILTKAPSAELKPGQVDQDSL
PPYEILDDILQRFIHNHQSVGEIVAAGHEPVVVDRVIQMVARAEFKRRQAPPGLKITDRAFGTGWRMPIASNWNAIKNNY
RLSYTF

Sequences:

>Translated_566_residues
MKIAIAQINPIIGDLTGNAQKILEIAQQAIKKGARLLLTPELSLCGYPPRDLLLNPSFVEAMSVTLQQLARDLPVNLAVL
VGTVEPNHQAHTTGGKPLFNSTALLENGKVKQMFHKRLLPTYDVFDEHRYFEAGQQANYFALDNINIGVTICEDLWNDEE
FWGKRSYTANPISDLAILGVDLIVNLSASPYSLGKQSFREAMLRHSALRFQQPVIYTNQVGGNDDLIFDGRSFALNRQGE
VMCRAKGFASDLITVDFDESQRDLQLNSVAPVYESEDEEIWHALVLGVRDYAQKCRFSQVVLGLSGGIDSALVATIATAA
LGKENVFGVLMPSPYSSEHSISDALALADNLGIKTQILPIGELMQSFDQSLVELFAGTEFGLAEENIQSRIRGNLLMAIA
NKFGYLLLSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKTRVYSLCNWLNSHQSPIIPENILTKAPSAELKPGQVDQDSL
PPYEILDDILQRFIHNHQSVGEIVAAGHEPVVVDRVIQMVARAEFKRRQAPPGLKITDRAFGTGWRMPIASNWNAIKNNY
RLSYTF
>Mature_566_residues
MKIAIAQINPIIGDLTGNAQKILEIAQQAIKKGARLLLTPELSLCGYPPRDLLLNPSFVEAMSVTLQQLARDLPVNLAVL
VGTVEPNHQAHTTGGKPLFNSTALLENGKVKQMFHKRLLPTYDVFDEHRYFEAGQQANYFALDNINIGVTICEDLWNDEE
FWGKRSYTANPISDLAILGVDLIVNLSASPYSLGKQSFREAMLRHSALRFQQPVIYTNQVGGNDDLIFDGRSFALNRQGE
VMCRAKGFASDLITVDFDESQRDLQLNSVAPVYESEDEEIWHALVLGVRDYAQKCRFSQVVLGLSGGIDSALVATIATAA
LGKENVFGVLMPSPYSSEHSISDALALADNLGIKTQILPIGELMQSFDQSLVELFAGTEFGLAEENIQSRIRGNLLMAIA
NKFGYLLLSTGNKSEMAVGYCTLYGDMNGGLAVIADVPKTRVYSLCNWLNSHQSPIIPENILTKAPSAELKPGQVDQDSL
PPYEILDDILQRFIHNHQSVGEIVAAGHEPVVVDRVIQMVARAEFKRRQAPPGLKITDRAFGTGWRMPIASNWNAIKNNY
RLSYTF

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62442; Mature: 62442

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIAQINPIIGDLTGNAQKILEIAQQAIKKGARLLLTPELSLCGYPPRDLLLNPSFVE
CEEEEEECCCHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHEECCHHHH
AMSVTLQQLARDLPVNLAVLVGTVEPNHQAHTTGGKPLFNSTALLENGKVKQMFHKRLLP
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCC
TYDVFDEHRYFEAGQQANYFALDNINIGVTICEDLWNDEEFWGKRSYTANPISDLAILGV
CHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHCCCHHHCCCCCCCCCCHHHHHHHEE
DLIVNLSASPYSLGKQSFREAMLRHSALRFQQPVIYTNQVGGNDDLIFDGRSFALNRQGE
EHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCEEECCCCC
VMCRAKGFASDLITVDFDESQRDLQLNSVAPVYESEDEEIWHALVLGVRDYAQKCRFSQV
EEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
VLGLSGGIDSALVATIATAALGKENVFGVLMPSPYSSEHSISDALALADNLGIKTQILPI
HEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECH
GELMQSFDQSLVELFAGTEFGLAEENIQSRIRGNLLMAIANKFGYLLLSTGNKSEMAVGY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHEEHHHCCEEEEECCCCCCEEEEE
CTLYGDMNGGLAVIADVPKTRVYSLCNWLNSHQSPIIPENILTKAPSAELKPGQVDQDSL
EEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
PPYEILDDILQRFIHNHQSVGEIVAAGHEPVVVDRVIQMVARAEFKRRQAPPGLKITDRA
CCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
FGTGWRMPIASNWNAIKNNYRLSYTF
CCCCCCCCCCCCCHHHCCCEEEEEEC
>Mature Secondary Structure
MKIAIAQINPIIGDLTGNAQKILEIAQQAIKKGARLLLTPELSLCGYPPRDLLLNPSFVE
CEEEEEECCCHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHEECCHHHH
AMSVTLQQLARDLPVNLAVLVGTVEPNHQAHTTGGKPLFNSTALLENGKVKQMFHKRLLP
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCC
TYDVFDEHRYFEAGQQANYFALDNINIGVTICEDLWNDEEFWGKRSYTANPISDLAILGV
CHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHCCCHHHCCCCCCCCCCHHHHHHHEE
DLIVNLSASPYSLGKQSFREAMLRHSALRFQQPVIYTNQVGGNDDLIFDGRSFALNRQGE
EHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCEEECCCCC
VMCRAKGFASDLITVDFDESQRDLQLNSVAPVYESEDEEIWHALVLGVRDYAQKCRFSQV
EEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
VLGLSGGIDSALVATIATAALGKENVFGVLMPSPYSSEHSISDALALADNLGIKTQILPI
HEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECH
GELMQSFDQSLVELFAGTEFGLAEENIQSRIRGNLLMAIANKFGYLLLSTGNKSEMAVGY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHEEHHHCCEEEEECCCCCCEEEEE
CTLYGDMNGGLAVIADVPKTRVYSLCNWLNSHQSPIIPENILTKAPSAELKPGQVDQDSL
EEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
PPYEILDDILQRFIHNHQSVGEIVAAGHEPVVVDRVIQMVARAEFKRRQAPPGLKITDRA
CCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
FGTGWRMPIASNWNAIKNNYRLSYTF
CCCCCCCCCCCCCHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]