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The map label for this gene is rimO [H]

Identifier: 17228717

GI number: 17228717

Start: 1442268

End: 1443590

Strand: Direct

Name: rimO [H]

Synonym: alr1222

Alternate gene names: 17228717

Gene position: 1442268-1443590 (Clockwise)

Preceding gene: 17228712

Following gene: 17228718

Centisome position: 22.49

GC content: 45.88

Gene sequence:

>1323_bases
ATGGGTGAAAAGCCGACAATAGCAATTTCTCACTTGGGCTGTGAGAAAAACCGAATTGATACAGAACATATGTTAGGCCT
GCTGGTAAAAGCAGGTTATGGTGTAGATACAAATGAAGAATTAGCCGACTACGTAATAGTCAATACTTGTAGTTTTATCG
AATCAGCCAGGGAAGAATCGGTTAGAACCTTAGTAGAACTCGCAGAAGCCAACAAAAAAATTGTAATTACGGGCTGTATG
GCGCAGCACTTCCAAACACAATTATTGGAAGAGCTACCCGAAGCAGTGGCTGTAGTTGGTACAGGGGACTACCATAAAAT
AGTTAGCGTCATTGAGCGAGCAGAGCAAGGCGAACGGGTTACACTAGTTAGCGCCGAGCCAACCTATATCGCCGATGAAA
CGACCCCACGCTATCGAACTACTACCGAAGGTGTAGCCTACCTGCGAGTAGCCGAAGGGTGTGATTACCGTTGTGCATTT
TGCATAATTCCCCACTTACGCGGAAACCAGCGATCGCGTACTATAGAATCTATAGTTGCTGAAGCCGAGCAACTAGTAGC
CCAAGGGGTGCAAGAGATCATTCTTATTTCCCAAATCACCACCAACTATGGGTTGGATATTTATGGTAAGCCCAAATTAG
CCGAACTATTGCGTGCTTTAGGGAAAATTAATGTACCGTGGATTAGGATGCACTACGCCTATCCCACTGGATTAACCCCG
GATGTGATAGCGGCAATTCAAGAAACGCCAAACGTCTTACCATATCTAGATTTGCCTCTACAACATTCTCATAGTGAAGT
TCTTCGTAGCATGAACCGTCCCTGGCAAGGGCGAGTCAACGATGAAATCATCGAGCGCCTCAAGATAGCCATTCCAGGTG
CTGTGTTACGGACAACATTTATAGTGGGCTTCCCTGGAGAAACAGAAGCACAGTTTGAGCATTTACTACAGTTCGTCCAA
CGGCATGAGTTTGATCATGTAGGTGTTTTTACCTTTTCCGCAGAAGAGGGAACCCCCGCTTACAAGTTACCAAATCAACT
GCCCCAAGAGGTGATGGATGAACGCCGCGATCGCCTCATGGCACTCCAGCAACCCATTTCTTGGCGAAAAAATCAGCAGG
AAGTCGGCAAAACCGTTGAAGTCCTGATTGAGCAAGAAAATCCTGAAAGTGGGAAATTAATTGGTCGTTCTGGCAGATTT
TCTCCAGAAGTTGATGGTCAGGTCTACGTTGATGGCGAGGCGAAGCTAGGAACCATTATCCCTGTGAAAATTCACAGTGC
TGATGAGTATGACCTGTTTGGTCAAGTTGTCAGTCATAACTGA

Upstream 100 bases:

>100_bases
CCCCACCAGATCGCTGTCAGTCAAGGGTGCGGAGGAAAAAAGCAAGACTATCGCGTTTTGTGTTTAATAACAAGAAAAGA
CTGGAAGTTTTTTTCAAAAT

Downstream 100 bases:

>100_bases
TAGCTGATCAGTAGTACAACGTTTCGGTTTACAAGTCTAAAAGTAAAAAAGCAAAATCTAGGAGAGTTGATGAACTTATC
GTTTCCCGAACTAGGAATTT

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MGEKPTIAISHLGCEKNRIDTEHMLGLLVKAGYGVDTNEELADYVIVNTCSFIESAREESVRTLVELAEANKKIVITGCM
AQHFQTQLLEELPEAVAVVGTGDYHKIVSVIERAEQGERVTLVSAEPTYIADETTPRYRTTTEGVAYLRVAEGCDYRCAF
CIIPHLRGNQRSRTIESIVAEAEQLVAQGVQEIILISQITTNYGLDIYGKPKLAELLRALGKINVPWIRMHYAYPTGLTP
DVIAAIQETPNVLPYLDLPLQHSHSEVLRSMNRPWQGRVNDEIIERLKIAIPGAVLRTTFIVGFPGETEAQFEHLLQFVQ
RHEFDHVGVFTFSAEEGTPAYKLPNQLPQEVMDERRDRLMALQQPISWRKNQQEVGKTVEVLIEQENPESGKLIGRSGRF
SPEVDGQVYVDGEAKLGTIIPVKIHSADEYDLFGQVVSHN

Sequences:

>Translated_440_residues
MGEKPTIAISHLGCEKNRIDTEHMLGLLVKAGYGVDTNEELADYVIVNTCSFIESAREESVRTLVELAEANKKIVITGCM
AQHFQTQLLEELPEAVAVVGTGDYHKIVSVIERAEQGERVTLVSAEPTYIADETTPRYRTTTEGVAYLRVAEGCDYRCAF
CIIPHLRGNQRSRTIESIVAEAEQLVAQGVQEIILISQITTNYGLDIYGKPKLAELLRALGKINVPWIRMHYAYPTGLTP
DVIAAIQETPNVLPYLDLPLQHSHSEVLRSMNRPWQGRVNDEIIERLKIAIPGAVLRTTFIVGFPGETEAQFEHLLQFVQ
RHEFDHVGVFTFSAEEGTPAYKLPNQLPQEVMDERRDRLMALQQPISWRKNQQEVGKTVEVLIEQENPESGKLIGRSGRF
SPEVDGQVYVDGEAKLGTIIPVKIHSADEYDLFGQVVSHN
>Mature_439_residues
GEKPTIAISHLGCEKNRIDTEHMLGLLVKAGYGVDTNEELADYVIVNTCSFIESAREESVRTLVELAEANKKIVITGCMA
QHFQTQLLEELPEAVAVVGTGDYHKIVSVIERAEQGERVTLVSAEPTYIADETTPRYRTTTEGVAYLRVAEGCDYRCAFC
IIPHLRGNQRSRTIESIVAEAEQLVAQGVQEIILISQITTNYGLDIYGKPKLAELLRALGKINVPWIRMHYAYPTGLTPD
VIAAIQETPNVLPYLDLPLQHSHSEVLRSMNRPWQGRVNDEIIERLKIAIPGAVLRTTFIVGFPGETEAQFEHLLQFVQR
HEFDHVGVFTFSAEEGTPAYKLPNQLPQEVMDERRDRLMALQQPISWRKNQQEVGKTVEVLIEQENPESGKLIGRSGRFS
PEVDGQVYVDGEAKLGTIIPVKIHSADEYDLFGQVVSHN

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=491, Percent_Identity=27.2912423625255, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI28872782, Length=491, Percent_Identity=27.0875763747454, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=28.0623608017817, Blast_Score=161, Evalue=9e-40,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=39.8230088495575, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=30.2690582959641, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=419, Percent_Identity=27.2076372315036, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71996771, Length=375, Percent_Identity=24.5333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356207, Length=449, Percent_Identity=26.7260579064588, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=26.8292682926829, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49270; Mature: 49139

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKPTIAISHLGCEKNRIDTEHMLGLLVKAGYGVDTNEELADYVIVNTCSFIESAREES
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRTLVELAEANKKIVITGCMAQHFQTQLLEELPEAVAVVGTGDYHKIVSVIERAEQGERV
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCEE
TLVSAEPTYIADETTPRYRTTTEGVAYLRVAEGCDYRCAFCIIPHLRGNQRSRTIESIVA
EEEECCCCEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHH
EAEQLVAQGVQEIILISQITTNYGLDIYGKPKLAELLRALGKINVPWIRMHYAYPTGLTP
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCH
DVIAAIQETPNVLPYLDLPLQHSHSEVLRSMNRPWQGRVNDEIIERLKIAIPGAVLRTTF
HHHHHHHHCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHEEEE
IVGFPGETEAQFEHLLQFVQRHEFDHVGVFTFSAEEGTPAYKLPNQLPQEVMDERRDRLM
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
ALQQPISWRKNQQEVGKTVEVLIEQENPESGKLIGRSGRFSPEVDGQVYVDGEAKLGTII
HHHCCHHHCCCHHHHHHHEEEEEECCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCEEE
PVKIHSADEYDLFGQVVSHN
EEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
GEKPTIAISHLGCEKNRIDTEHMLGLLVKAGYGVDTNEELADYVIVNTCSFIESAREES
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRTLVELAEANKKIVITGCMAQHFQTQLLEELPEAVAVVGTGDYHKIVSVIERAEQGERV
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCEE
TLVSAEPTYIADETTPRYRTTTEGVAYLRVAEGCDYRCAFCIIPHLRGNQRSRTIESIVA
EEEECCCCEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHH
EAEQLVAQGVQEIILISQITTNYGLDIYGKPKLAELLRALGKINVPWIRMHYAYPTGLTP
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCH
DVIAAIQETPNVLPYLDLPLQHSHSEVLRSMNRPWQGRVNDEIIERLKIAIPGAVLRTTF
HHHHHHHHCCCCCCEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHEEEE
IVGFPGETEAQFEHLLQFVQRHEFDHVGVFTFSAEEGTPAYKLPNQLPQEVMDERRDRLM
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
ALQQPISWRKNQQEVGKTVEVLIEQENPESGKLIGRSGRFSPEVDGQVYVDGEAKLGTII
HHHCCHHHCCCHHHHHHHEEEEEECCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCEEE
PVKIHSADEYDLFGQVVSHN
EEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA