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The map label for this gene is ndhF [H]

Identifier: 17228364

GI number: 17228364

Start: 997980

End: 999836

Strand: Direct

Name: ndhF [H]

Synonym: alr0869

Alternate gene names: 17228364

Gene position: 997980-999836 (Clockwise)

Preceding gene: 17228352

Following gene: 17228365

Centisome position: 15.56

GC content: 44.96

Gene sequence:

>1857_bases
ATGAATCAGTTTCTATTTGCAACAAGTTGGTGTGTGCCTTTTTACAGCTTGCTAGGCGCACTGTTGACATTACCTTGGGG
AATAGGAATAGTTCGACGCACTGGGCCTAGGCCTGCGGCGTATTTTAACTTGTTGACTACCATTGTGGGCTTTGCTCATA
GCCTATGGGTATTTAAGGATATTTGGAGCAGAGAACAAGAAAATTTAGTGATTACTTGGTTTCAAGCTGCGGATTTAAAC
TTATCCTTTGCGTTGGAACTCTCACCTGTCAGCATGGGAGCGACAGTATTAATCACAGGGTTAAGTTTGTTAGCGCAAAT
TTATGCCTTGGGTTACATGGAGAAAGACTGGTCATTGGCGCGTTTTTTCGGTCTGCTCGGCTTTTTTGAAGCAGCACTGA
GCGGTTTAGCTATTAGCGACTCTTTATTTCTCAGCTATGGACTATTGGAAATATTGACGCTTTCCACTTACTTACTTGTA
GGTTTTTGGTACGCTCAACCGCTAGTGGTAACAGCTGCACGGGATGCTTTTTGGACAAAGCGCGTGGGTGACTTGTTGCT
GTTGATGGCTGTAGTCACACTTTCCACCTTAGCGGGTAGTTTGAACTTTTCAGATTTATATGAGTGGGTACAAACTGCGA
ATTTAGACCCCGTAACTGCAACATTGTTGTGTTTAGGGTTGATTGCGGGCCCGGCTGGTAAATGCGCTCAGTTCCCCTTG
CATCTGTGGTTGGATGAGGCTATGGAAGGGCCCAACCCAGCATCTGTGATGCGGAACTCACTGGTGGTTGCTGGTGGTGC
TTATCTATTATACAAACTACAACCAATTTTAATTCTGTCGCCAGTGGCGTTGAATGTGCTGATTATCATTGGTGGGGTGA
CAGCAATTGGTGCTAGTTTGGTTTCCATTGCTCAGACTGATATTAAGCGGGCTTTGTCTCATTCCACCAGTGCATATATG
GGTTTGGTTTTTCTGGCGGTGGGCTTAGAACAGGGTGGTGTAGCTTTGATGCTGTTGTTGACCCATGCGATCGCCAAAGC
GTTACTATTTATGAGTTCGGGTTCTGTCATATTCACGACTCATAGTCAAGACTTGACAGAGATGGGTGGTTTATGGTCAC
GGATGCCAGCTACTACGACTGCCTTTGTGGTTGGTTCTGCCGGGATGGTGACACTGTTACCACTAGGTAGCTTTTGGGCG
ATGTTGTCTTGGGCTGACGGTTTGGTAAGAGTTAGCCCTTGGGTAATTGGAGTTTTAATTTTAGTCAATGGTTTGACGGC
TTTGAACTTGACGCGAGTATTTAGACTGGCATTTTGGGGTCAACCGCAACAAAAGACCCGCCGTGCGCCAGAGGTTGGCT
GGACAATGGCTTTCCCGATGGTGACATTGATAATATTGACTTTATTATTACCCCTGATGTTACAGCAGTGGTACTTATTA
CCAGCTTGGGAAAGTATTGACTGGTACGTTGTTTTAGTTTTGGTTTCCTCTACGGTGGCTGGTGTAGTGATTGGGTCAAC
GATTCATTTACACAAAGCTTGGTCTCGTTCCACAGTTTTGGCTTGGAGATTCATACAGGATTTATTGGGTTATGACTTCT
ACATTGACCGCATTTATCGCTTGACAGTTGTGAGTGCAGTAGCTTTATTGTCGAGGATTTCTGCTTGGAGCGATCGCTAT
TTAGTTGATGGTTTAGTGAACTTAGTGGGCTTTGCCACAATTTTCGGCGGGCAAGGTTTGAAATATAGCATTTCCGGTCA
ATCCCAAGGATATATGTTGACTATCCTCGCCGTAGTTGGTGCTTTAGGCTTTTTCATTAGCTGGTCATTAGGCTTACTGG
ATAAATTGCCTTTTTAG

Upstream 100 bases:

>100_bases
TTAAAAAAAGCATATTTTTTGGCAAATTTTATTTTTTTTAGTAAATGATTGTTTTAAGAGTTTTAGAAAAGTTAAGGCTG
AGTCTTCAAGGGAAATCTAA

Downstream 100 bases:

>100_bases
TCAGTTGTCAATAGAAGAGACGTGCATACAACGTCTGTACATTAATTCTTCCCCCTGCCTAAACTTCCTCATTTCCCCAT
GATTATCTATGCTTAGTGTA

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MNQFLFATSWCVPFYSLLGALLTLPWGIGIVRRTGPRPAAYFNLLTTIVGFAHSLWVFKDIWSREQENLVITWFQAADLN
LSFALELSPVSMGATVLITGLSLLAQIYALGYMEKDWSLARFFGLLGFFEAALSGLAISDSLFLSYGLLEILTLSTYLLV
GFWYAQPLVVTAARDAFWTKRVGDLLLLMAVVTLSTLAGSLNFSDLYEWVQTANLDPVTATLLCLGLIAGPAGKCAQFPL
HLWLDEAMEGPNPASVMRNSLVVAGGAYLLYKLQPILILSPVALNVLIIIGGVTAIGASLVSIAQTDIKRALSHSTSAYM
GLVFLAVGLEQGGVALMLLLTHAIAKALLFMSSGSVIFTTHSQDLTEMGGLWSRMPATTTAFVVGSAGMVTLLPLGSFWA
MLSWADGLVRVSPWVIGVLILVNGLTALNLTRVFRLAFWGQPQQKTRRAPEVGWTMAFPMVTLIILTLLLPLMLQQWYLL
PAWESIDWYVVLVLVSSTVAGVVIGSTIHLHKAWSRSTVLAWRFIQDLLGYDFYIDRIYRLTVVSAVALLSRISAWSDRY
LVDGLVNLVGFATIFGGQGLKYSISGQSQGYMLTILAVVGALGFFISWSLGLLDKLPF

Sequences:

>Translated_618_residues
MNQFLFATSWCVPFYSLLGALLTLPWGIGIVRRTGPRPAAYFNLLTTIVGFAHSLWVFKDIWSREQENLVITWFQAADLN
LSFALELSPVSMGATVLITGLSLLAQIYALGYMEKDWSLARFFGLLGFFEAALSGLAISDSLFLSYGLLEILTLSTYLLV
GFWYAQPLVVTAARDAFWTKRVGDLLLLMAVVTLSTLAGSLNFSDLYEWVQTANLDPVTATLLCLGLIAGPAGKCAQFPL
HLWLDEAMEGPNPASVMRNSLVVAGGAYLLYKLQPILILSPVALNVLIIIGGVTAIGASLVSIAQTDIKRALSHSTSAYM
GLVFLAVGLEQGGVALMLLLTHAIAKALLFMSSGSVIFTTHSQDLTEMGGLWSRMPATTTAFVVGSAGMVTLLPLGSFWA
MLSWADGLVRVSPWVIGVLILVNGLTALNLTRVFRLAFWGQPQQKTRRAPEVGWTMAFPMVTLIILTLLLPLMLQQWYLL
PAWESIDWYVVLVLVSSTVAGVVIGSTIHLHKAWSRSTVLAWRFIQDLLGYDFYIDRIYRLTVVSAVALLSRISAWSDRY
LVDGLVNLVGFATIFGGQGLKYSISGQSQGYMLTILAVVGALGFFISWSLGLLDKLPF
>Mature_618_residues
MNQFLFATSWCVPFYSLLGALLTLPWGIGIVRRTGPRPAAYFNLLTTIVGFAHSLWVFKDIWSREQENLVITWFQAADLN
LSFALELSPVSMGATVLITGLSLLAQIYALGYMEKDWSLARFFGLLGFFEAALSGLAISDSLFLSYGLLEILTLSTYLLV
GFWYAQPLVVTAARDAFWTKRVGDLLLLMAVVTLSTLAGSLNFSDLYEWVQTANLDPVTATLLCLGLIAGPAGKCAQFPL
HLWLDEAMEGPNPASVMRNSLVVAGGAYLLYKLQPILILSPVALNVLIIIGGVTAIGASLVSIAQTDIKRALSHSTSAYM
GLVFLAVGLEQGGVALMLLLTHAIAKALLFMSSGSVIFTTHSQDLTEMGGLWSRMPATTTAFVVGSAGMVTLLPLGSFWA
MLSWADGLVRVSPWVIGVLILVNGLTALNLTRVFRLAFWGQPQQKTRRAPEVGWTMAFPMVTLIILTLLLPLMLQQWYLL
PAWESIDWYVVLVLVSSTVAGVVIGSTIHLHKAWSRSTVLAWRFIQDLLGYDFYIDRIYRLTVVSAVALLSRISAWSDRY
LVDGLVNLVGFATIFGGQGLKYSISGQSQGYMLTILAVVGALGFFISWSLGLLDKLPF

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=391, Percent_Identity=31.7135549872123, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI1788614, Length=405, Percent_Identity=35.0617283950617, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1788827, Length=403, Percent_Identity=28.287841191067, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788831, Length=400, Percent_Identity=28.25, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788829, Length=442, Percent_Identity=24.2081447963801, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788613, Length=280, Percent_Identity=27.1428571428571, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367154, Length=184, Percent_Identity=27.7173913043478, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 67439; Mature: 67439

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFLFATSWCVPFYSLLGALLTLPWGIGIVRRTGPRPAAYFNLLTTIVGFAHSLWVFKD
CCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IWSREQENLVITWFQAADLNLSFALELSPVSMGATVLITGLSLLAQIYALGYMEKDWSLA
HHCCCCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
RFFGLLGFFEAALSGLAISDSLFLSYGLLEILTLSTYLLVGFWYAQPLVVTAARDAFWTK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
RVGDLLLLMAVVTLSTLAGSLNFSDLYEWVQTANLDPVTATLLCLGLIAGPAGKCAQFPL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHCHH
HLWLDEAMEGPNPASVMRNSLVVAGGAYLLYKLQPILILSPVALNVLIIIGGVTAIGASL
HHHHHHHCCCCCHHHHHHCCEEEECCCEEEHHHCHHHEECHHHHHHHHHHHHHHHHHHHH
VSIAQTDIKRALSHSTSAYMGLVFLAVGLEQGGVALMLLLTHAIAKALLFMSSGSVIFTT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
HSQDLTEMGGLWSRMPATTTAFVVGSAGMVTLLPLGSFWAMLSWADGLVRVSPWVIGVLI
CCCHHHHHCCHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCCEEHHHHHHHHHH
LVNGLTALNLTRVFRLAFWGQPQQKTRRAPEVGWTMAFPMVTLIILTLLLPLMLQQWYLL
HHCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAWESIDWYVVLVLVSSTVAGVVIGSTIHLHKAWSRSTVLAWRFIQDLLGYDFYIDRIYR
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
LTVVSAVALLSRISAWSDRYLVDGLVNLVGFATIFGGQGLKYSISGQSQGYMLTILAVVG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
ALGFFISWSLGLLDKLPF
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQFLFATSWCVPFYSLLGALLTLPWGIGIVRRTGPRPAAYFNLLTTIVGFAHSLWVFKD
CCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IWSREQENLVITWFQAADLNLSFALELSPVSMGATVLITGLSLLAQIYALGYMEKDWSLA
HHCCCCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
RFFGLLGFFEAALSGLAISDSLFLSYGLLEILTLSTYLLVGFWYAQPLVVTAARDAFWTK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
RVGDLLLLMAVVTLSTLAGSLNFSDLYEWVQTANLDPVTATLLCLGLIAGPAGKCAQFPL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHCHH
HLWLDEAMEGPNPASVMRNSLVVAGGAYLLYKLQPILILSPVALNVLIIIGGVTAIGASL
HHHHHHHCCCCCHHHHHHCCEEEECCCEEEHHHCHHHEECHHHHHHHHHHHHHHHHHHHH
VSIAQTDIKRALSHSTSAYMGLVFLAVGLEQGGVALMLLLTHAIAKALLFMSSGSVIFTT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
HSQDLTEMGGLWSRMPATTTAFVVGSAGMVTLLPLGSFWAMLSWADGLVRVSPWVIGVLI
CCCHHHHHCCHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCCEEHHHHHHHHHH
LVNGLTALNLTRVFRLAFWGQPQQKTRRAPEVGWTMAFPMVTLIILTLLLPLMLQQWYLL
HHCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAWESIDWYVVLVLVSSTVAGVVIGSTIHLHKAWSRSTVLAWRFIQDLLGYDFYIDRIYR
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
LTVVSAVALLSRISAWSDRYLVDGLVNLVGFATIFGGQGLKYSISGQSQGYMLTILAVVG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
ALGFFISWSLGLLDKLPF
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]