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The map label for this gene is rfaB

Identifier: 16767004

GI number: 16767004

Start: 3914534

End: 3915613

Strand: Reverse

Name: rfaB

Synonym: STM3719

Alternate gene names: 16767004

Gene position: 3915613-3914534 (Counterclockwise)

Preceding gene: 16767005

Following gene: 16767003

Centisome position: 80.61

GC content: 39.54

Gene sequence:

>1080_bases
ATGAAAATAGCTTTTATTGGCGAAGCGGTATCCGGTTTTGGAGGGATGGAAACCGTTATTAGCAACGTTATCCATACGTT
TGAAAATAGCTCTCCGAAGATCAACTGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAAGCATGGCTAAAAG
AGATTAAATATGCGCAATCATTCTCAAATATAAAATTAAGTTTTCTTCGTCGAGCTAAGCATGTTTATAATTTTAGTCAG
TGGCTGAAAGAAACGTCACCGGATATTGTGATATGTATTGATGTCATCTCATGTCTTTATGCCAATAAAGCACGAAAGAA
AAGTGGTAAACATTTTACTATTTTCTCGTGGCCGCATTTTTCGCTCGACCATAAAAAACATGCAGAATGTATAACGTACG
CGGATTATCATCTGGCCATCAGTAGTGGTATTAAAGAACAGATCATGGCACGGGGTATCTCAGCGCAAGATATTAGCGTC
GTTTATAATCCTGTCTCTATTAAAACAGTCATTGTGCCGCCGCCTGAGCGCGATAAACCCGCTGTATTTCTCTATGTAGG
GCGTCTTAAATTTGAAGGGCAGAAAAGAGTTAAAGATTTATTTGATGGCTTAGCTCGTACGACAGGGGAATGGCAGCTAC
ATATTATTGGTGATGGCTCAGATTTTGAAAAGTGCCAGGCCTATAGTCGGGAGCTTGGTATTGAGCAGCGTGTGATCTGG
TATGGTTGGCAAAGCGCGCCGTGGCAAGTCGTACAGCAAAAAATAAAAAATGTCACCGCGTTACTACTTACCTCTGCATT
TGAGGGATTTCCTATGACCCTGCTGGAAGCAATGTCATATGGAATTCCGTGTATTAGTTCTGATTGTATGTCTGGTCCGC
GCGATATGATTAAACCCGGTTTGAATGGTGAGCTTTACACTCCGGGTGCTATAGATGATTTTGTAGGACACCTTAATCGC
GTTATTTCTGGTGAAGTAAAATATCAGCATGATATTATTCCCGGCACGATTGAGAGATTTTACGATGTGCTATATTTTAA
AAATTTTAATAATGCAATATTCTCGAAATTACAAAAGTGA

Upstream 100 bases:

>100_bases
AGAGAGATGTCAGGTAAAAATTACTCTATAGGGTTATATTACTATTTTTGCTATTTAATATCTAAAGTCTTTAGATTAAG
GTTTTAACTCTGGGGTATAT

Downstream 100 bases:

>100_bases
TCACTATGAGCAGAAAATATTTTGAAGAAGAAGTCATTCAACAGACTTTAGATTATAACTATGCACAACATAGTGATGCT
GATAAATTTAATATAGCTTA

Product: UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase

Products: NA

Alternate protein names: UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKIAFIGEAVSGFGGMETVISNVIHTFENSSPKINCEMFFFCRNDKMDKAWLKEIKYAQSFSNIKLSFLRRAKHVYNFSQ
WLKETSPDIVICIDVISCLYANKARKKSGKHFTIFSWPHFSLDHKKHAECITYADYHLAISSGIKEQIMARGISAQDISV
VYNPVSIKTVIVPPPERDKPAVFLYVGRLKFEGQKRVKDLFDGLARTTGEWQLHIIGDGSDFEKCQAYSRELGIEQRVIW
YGWQSAPWQVVQQKIKNVTALLLTSAFEGFPMTLLEAMSYGIPCISSDCMSGPRDMIKPGLNGELYTPGAIDDFVGHLNR
VISGEVKYQHDIIPGTIERFYDVLYFKNFNNAIFSKLQK

Sequences:

>Translated_359_residues
MKIAFIGEAVSGFGGMETVISNVIHTFENSSPKINCEMFFFCRNDKMDKAWLKEIKYAQSFSNIKLSFLRRAKHVYNFSQ
WLKETSPDIVICIDVISCLYANKARKKSGKHFTIFSWPHFSLDHKKHAECITYADYHLAISSGIKEQIMARGISAQDISV
VYNPVSIKTVIVPPPERDKPAVFLYVGRLKFEGQKRVKDLFDGLARTTGEWQLHIIGDGSDFEKCQAYSRELGIEQRVIW
YGWQSAPWQVVQQKIKNVTALLLTSAFEGFPMTLLEAMSYGIPCISSDCMSGPRDMIKPGLNGELYTPGAIDDFVGHLNR
VISGEVKYQHDIIPGTIERFYDVLYFKNFNNAIFSKLQK
>Mature_359_residues
MKIAFIGEAVSGFGGMETVISNVIHTFENSSPKINCEMFFFCRNDKMDKAWLKEIKYAQSFSNIKLSFLRRAKHVYNFSQ
WLKETSPDIVICIDVISCLYANKARKKSGKHFTIFSWPHFSLDHKKHAECITYADYHLAISSGIKEQIMARGISAQDISV
VYNPVSIKTVIVPPPERDKPAVFLYVGRLKFEGQKRVKDLFDGLARTTGEWQLHIIGDGSDFEKCQAYSRELGIEQRVIW
YGWQSAPWQVVQQKIKNVTALLLTSAFEGFPMTLLEAMSYGIPCISSDCMSGPRDMIKPGLNGELYTPGAIDDFVGHLNR
VISGEVKYQHDIIPGTIERFYDVLYFKNFNNAIFSKLQK

Specific function: Adds a galactose goup to a glucose group of LPS

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

Organism=Escherichia coli, GI226510984, Length=359, Percent_Identity=64.066852367688, Blast_Score=501, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAB_SALTY (Q06994)

Other databases:

- EMBL:   AF026386
- EMBL:   AE006468
- EMBL:   S56361
- PIR:   C47074
- RefSeq:   NP_462619.1
- ProteinModelPortal:   Q06994
- PRIDE:   Q06994
- GeneID:   1255243
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3719
- NMPDR:   fig|99287.1.peg.3595
- HOGENOM:   HBG467988
- OMA:   EQIMARG
- ProtClustDB:   PRK09922
- BioCyc:   STYP99287:STM3719-MONOMER
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: 2.4.1.-

Molecular weight: Translated: 40891; Mature: 40891

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFIGEAVSGFGGMETVISNVIHTFENSSPKINCEMFFFCRNDKMDKAWLKEIKYAQS
CEEEEECHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
FSNIKLSFLRRAKHVYNFSQWLKETSPDIVICIDVISCLYANKARKKSGKHFTIFSWPHF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCC
SLDHKKHAECITYADYHLAISSGIKEQIMARGISAQDISVVYNPVSIKTVIVPPPERDKP
CCCCHHCCCCEEECHHHHHHHHCHHHHHHHCCCCCCCEEEEECCEEEEEEEECCCCCCCC
AVFLYVGRLKFEGQKRVKDLFDGLARTTGEWQLHIIGDGSDFEKCQAYSRELGIEQRVIW
EEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEE
YGWQSAPWQVVQQKIKNVTALLLTSAFEGFPMTLLEAMSYGIPCISSDCMSGPRDMIKPG
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCCCCHHHHCCC
LNGELYTPGAIDDFVGHLNRVISGEVKYQHDIIPGTIERFYDVLYFKNFNNAIFSKLQK
CCCCEECCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MKIAFIGEAVSGFGGMETVISNVIHTFENSSPKINCEMFFFCRNDKMDKAWLKEIKYAQS
CEEEEECHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHH
FSNIKLSFLRRAKHVYNFSQWLKETSPDIVICIDVISCLYANKARKKSGKHFTIFSWPHF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCC
SLDHKKHAECITYADYHLAISSGIKEQIMARGISAQDISVVYNPVSIKTVIVPPPERDKP
CCCCHHCCCCEEECHHHHHHHHCHHHHHHHCCCCCCCEEEEECCEEEEEEEECCCCCCCC
AVFLYVGRLKFEGQKRVKDLFDGLARTTGEWQLHIIGDGSDFEKCQAYSRELGIEQRVIW
EEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEE
YGWQSAPWQVVQQKIKNVTALLLTSAFEGFPMTLLEAMSYGIPCISSDCMSGPRDMIKPG
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCCCCHHHHCCC
LNGELYTPGAIDDFVGHLNRVISGEVKYQHDIIPGTIERFYDVLYFKNFNNAIFSKLQK
CCCCEECCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535865; 11677609; 8444813