Click here to switch to the map view.

The map label for this gene is rfaJ

Identifier: 16767002

GI number: 16767002

Start: 3912487

End: 3913497

Strand: Reverse

Name: rfaJ

Synonym: STM3717

Alternate gene names: 16767002

Gene position: 3913497-3912487 (Counterclockwise)

Preceding gene: 16767003

Following gene: 16767001

Centisome position: 80.57

GC content: 34.62

Gene sequence:

>1011_bases
ATGGATTCATTTCCTGAGATAGAAATAGCTGAATATAAAGTTTTTGATGAAAGTAATAATAATGATGATAACGTATTAAA
CATTTCTTATGGCGTTGATGAAAACTATCTTGATGGTGTGGGGGTATCAATCGCTTCAGTTGTATTAAACAATAATATCC
CGCTCGCTTTTCACATTATTTGTGACTCATACTCCCCGTGTTTTGTAAAATATATAGAGCGTTTAGCCGTACAGCATCAC
ATAAAAATTTCTCTTTATCTTATTAAAGTAGAAAGCCTTGAGGTATTGCCTCAAACTAAAGTATGGTCGAGAGCAATGTA
TTTTCGTTTATTTGCTTTCGATTATCTCAGCAAGAAGGTAAATACCTTACTTTATTTGGATGCCGATGTTGTATGCAAAG
GATCTTTGCAAGATCTTTTACAGCTTGATCTGACAGAGAAGATCGCTGCGGTCGTAAAAGATGTTGATTCCATCCAGAAT
AAGGTAAATGAGAGATTAAGCGCTTTTAATTTACAAGGTGGTTATTTTAACTCCGGCGTGGTTTTTGTTAACCTGAAATT
ATGGAAAGAGAATGCCTTAACCAAAAAGGCATTTTTACTTTTGGCAGGTAAAGAGGCTGACTCTTTTAAATATCCCGATC
AGGATGTTTTGAATATTCTCCTACAGGATAAAGTCATTTTTCTACCGCGACCGTATAATACTATTTATACTATTAAAAGT
GAGTTGAAAGATAAGTCACATAAAAAATATAGCAATATAATTAATGATAATACTATTTTAATTCATTATACGGGCGCTAC
AAAACCATGGCATGCCTGGGCAAATTATCCTTCAGTTATCTATTATAAAAATGCACGACTGAACTCGCCCTGGAAAGATT
TTCCCGCAAAAGATGCGCGTACCATAGTCGAATTTAAGAAGCGATATAAACATCTTCTCGTGCAAGGTCATTATTTTAAA
GGCCTTCTGGCTGGAAGCGCATATCTTTATCGTAAACTTTTCCACAAATAA

Upstream 100 bases:

>100_bases
CAAAGCATATGTTTAATCAGAAGCATTATACTTCGGGTATAAATTATTATATAGCCTACTTTAAACGTAAACTTCTTGAA
TAAAACCCATAGGTGATGTA

Downstream 100 bases:

>100_bases
TTCCCCTCCCTAAATCTCCATTATGATTATTGAAAAAAAGATTAAAAACTATACCGTCTTTGTCAAAAAAGACGGTGAAA
AATACATTGAGATATTCAAA

Product: lipopolysaccharide glucosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSPCFVKYIERLAVQHH
IKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQN
KVNERLSAFNLQGGYFNSGVVFVNLKLWKENALTKKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDARTIVEFKKRYKHLLVQGHYFK
GLLAGSAYLYRKLFHK

Sequences:

>Translated_336_residues
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSPCFVKYIERLAVQHH
IKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQN
KVNERLSAFNLQGGYFNSGVVFVNLKLWKENALTKKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDARTIVEFKKRYKHLLVQGHYFK
GLLAGSAYLYRKLFHK
>Mature_336_residues
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHIICDSYSPCFVKYIERLAVQHH
IKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKVNTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQN
KVNERLSAFNLQGGYFNSGVVFVNLKLWKENALTKKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDARTIVEFKKRYKHLLVQGHYFK
GLLAGSAYLYRKLFHK

Specific function: Adds the glucose(II) group on the galactose(I) group of LPS

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family

Homologues:

Organism=Escherichia coli, GI1790056, Length=339, Percent_Identity=58.4070796460177, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790057, Length=326, Percent_Identity=35.5828220858896, Blast_Score=200, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAJ_SALTY (P19817)

Other databases:

- EMBL:   X53847
- EMBL:   AF026386
- EMBL:   AE006468
- PIR:   S12098
- RefSeq:   NP_462617.1
- ProteinModelPortal:   P19817
- SMR:   P19817
- PRIDE:   P19817
- GeneID:   1255241
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3717
- NMPDR:   fig|99287.1.peg.3593
- HOGENOM:   HBG417202
- OMA:   DRSHRKF
- ProtClustDB:   CLSK2484803
- BioCyc:   STYP99287:STM3717-MONOMER
- BRENDA:   2.4.1.58
- InterPro:   IPR002495
- InterPro:   IPR013645

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N

EC number: =2.4.1.58

Molecular weight: Translated: 38763; Mature: 38763

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHII
CCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEEEE
CDSYSPCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKV
ECCCCHHHHHHHHHHHHHHEEEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHHH
NTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLSAFNLQGGYFNSGV
CEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEECCCE
VFVNLKLWKENALTKKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
EEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEHH
ELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDAR
HHCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
TIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKLFHK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHII
CCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEEEE
CDSYSPCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKV
ECCCCHHHHHHHHHHHHHHEEEEEEEEEEEECEECCCCHHHHHHHHHHHHHHHHHHHHHH
NTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLSAFNLQGGYFNSGV
CEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEECCCE
VFVNLKLWKENALTKKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
EEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEHH
ELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDAR
HHCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCHHHH
TIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKLFHK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609