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The map label for this gene is rfaY

Identifier: 16767001

GI number: 16767001

Start: 3911766

End: 3912464

Strand: Reverse

Name: rfaY

Synonym: STM3716

Alternate gene names: 16767001

Gene position: 3912464-3911766 (Counterclockwise)

Preceding gene: 16767002

Following gene: 16767000

Centisome position: 80.55

GC content: 33.48

Gene sequence:

>699_bases
ATGATTATTGAAAAAAAGATTAAAAACTATACCGTCTTTGTCAAAAAAGACGGTGAAAAATACATTGAGATATTCAAAGA
TTTTCTATCTTATAATCATCAGGTTATTAAAGTCTTCCGTAATATAGAAGATACAAAAGTTGTATTGATTAATACAGACT
ACGGAAAATATATTCTCAAAGTATTTAGTCCAAAAGTAAAAAATACTGAACGCTTTTTCAAATCGTTAGTAAAAGGAGAT
TACTACGAAAAGCTTTTTCATCAGACAGATCGTGTGCGGCGTGAAGGGTTTGCGGCGCTTAATGATTTTTACCTGCTGGC
GGAAATTAAAACATTACGCTATGTAAAAACATACGTGATGATAATTGAGTATATTGAAGGTATTGAACTTGTTGATATGC
CAGAAATTTCTGACGAGGTGAGAGGGAAAATCAAACAGTCGATATATTCTCTGCATCAGCATGGTATGGTTTCGGGCGAT
CCCCATAAAGGTAACTTTATCCTGCAAGGCAATGAGATCAGAATTATCGATTTGTCCGGAAAAAGACCTTCCAGACAACG
AAAAGCAAAGGATCGTATTGATTTAGAAAGACATTATGGTATCAAAAATAATGTGAGAGATATCGGATTTTACTTATTGA
TTTATAAGAAAAAACTCCGAAATTTCTTGCGTCGTATTAAGGGTAAGGAAAAACGCTGA

Upstream 100 bases:

>100_bases
CTTCTCGTGCAAGGTCATTATTTTAAAGGCCTTCTGGCTGGAAGCGCATATCTTTATCGTAAACTTTTCCACAAATAATT
CCCCTCCCTAAATCTCCATT

Downstream 100 bases:

>100_bases
TCAATGTAGCACGTTTGGTTTAATTTAAGGGGCTGACTGACTTTTATTTGCTGCAGCGTTATGACATACTTGAGAGAATT
TGAGATGTAATATTAGTTAT

Product: lipopolysaccharide core biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIIEKKIKNYTVFVKKDGEKYIEIFKDFLSYNHQVIKVFRNIEDTKVVLINTDYGKYILKVFSPKVKNTERFFKSLVKGD
YYEKLFHQTDRVRREGFAALNDFYLLAEIKTLRYVKTYVMIIEYIEGIELVDMPEISDEVRGKIKQSIYSLHQHGMVSGD
PHKGNFILQGNEIRIIDLSGKRPSRQRKAKDRIDLERHYGIKNNVRDIGFYLLIYKKKLRNFLRRIKGKEKR

Sequences:

>Translated_232_residues
MIIEKKIKNYTVFVKKDGEKYIEIFKDFLSYNHQVIKVFRNIEDTKVVLINTDYGKYILKVFSPKVKNTERFFKSLVKGD
YYEKLFHQTDRVRREGFAALNDFYLLAEIKTLRYVKTYVMIIEYIEGIELVDMPEISDEVRGKIKQSIYSLHQHGMVSGD
PHKGNFILQGNEIRIIDLSGKRPSRQRKAKDRIDLERHYGIKNNVRDIGFYLLIYKKKLRNFLRRIKGKEKR
>Mature_232_residues
MIIEKKIKNYTVFVKKDGEKYIEIFKDFLSYNHQVIKVFRNIEDTKVVLINTDYGKYILKVFSPKVKNTERFFKSLVKGD
YYEKLFHQTDRVRREGFAALNDFYLLAEIKTLRYVKTYVMIIEYIEGIELVDMPEISDEVRGKIKQSIYSLHQHGMVSGD
PHKGNFILQGNEIRIIDLSGKRPSRQRKAKDRIDLERHYGIKNNVRDIGFYLLIYKKKLRNFLRRIKGKEKR

Specific function: Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. RfaY/waaY family

Homologues:

Organism=Escherichia coli, GI1790055, Length=232, Percent_Identity=69.8275862068966, Blast_Score=340, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAY_SALTY (P26472)

Other databases:

- EMBL:   AF001397
- EMBL:   AE006468
- EMBL:   AF026386
- EMBL:   M73826
- PIR:   E41317
- RefSeq:   NP_462616.1
- ProteinModelPortal:   P26472
- SMR:   P26472
- PRIDE:   P26472
- GeneID:   1255240
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3716
- HOGENOM:   HBG468926
- OMA:   MIIEKKV
- ProtClustDB:   PRK10359
- BioCyc:   STYP99287:STM3716-MONOMER
- InterPro:   IPR011009
- InterPro:   IPR009330

Pfam domain/function: PF06176 WaaY; SSF56112 Kinase_like

EC number: 2.7.-.-

Molecular weight: Translated: 27741; Mature: 27741

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEKKIKNYTVFVKKDGEKYIEIFKDFLSYNHQVIKVFRNIEDTKVVLINTDYGKYILK
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCHHHHHHH
VFSPKVKNTERFFKSLVKGDYYEKLFHQTDRVRREGFAALNDFYLLAEIKTLRYVKTYVM
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIEYIEGIELVDMPEISDEVRGKIKQSIYSLHQHGMVSGDPHKGNFILQGNEIRIIDLSG
HHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEECC
KRPSRQRKAKDRIDLERHYGIKNNVRDIGFYLLIYKKKLRNFLRRIKGKEKR
CCCCHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIIEKKIKNYTVFVKKDGEKYIEIFKDFLSYNHQVIKVFRNIEDTKVVLINTDYGKYILK
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCHHHHHHH
VFSPKVKNTERFFKSLVKGDYYEKLFHQTDRVRREGFAALNDFYLLAEIKTLRYVKTYVM
HHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIEYIEGIELVDMPEISDEVRGKIKQSIYSLHQHGMVSGDPHKGNFILQGNEIRIIDLSG
HHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEEEEECC
KRPSRQRKAKDRIDLERHYGIKNNVRDIGFYLLIYKKKLRNFLRRIKGKEKR
CCCCHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573095; 11677609; 9535865; 1657881