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The map label for this gene is amiC [H]

Identifier: 16766293

GI number: 16766293

Start: 3141356

End: 3142609

Strand: Reverse

Name: amiC [H]

Synonym: STM2991

Alternate gene names: 16766293

Gene position: 3142609-3141356 (Counterclockwise)

Preceding gene: 16766295

Following gene: 16766292

Centisome position: 64.7

GC content: 53.67

Gene sequence:

>1254_bases
ATGTCGGGAGCCAATTCAGCAATCAGCCGTCGCCGTTTACTACAAGGCGCGGGCGCCATGTGGCTATTAAGCGTGAGCCA
GGTCGGGCTGGCTGCCGTAAGCCAGGTCGTTGCGGTGCGTATCTGGCCTGCGTCCAGCTATACCCGCGTCACGGTAGAGT
CGAATCGTCTGCTGAAATATAAACAATTTGCCTTAAGTAACCCGGACCGCGTGGTAGTAGATATTGAGGATGTGAATCTG
AACTCGGTGCTTAAAGGCATTGGCGCGCAGATTCGCAGCGACGATCCGTATATCAAATCCGCAAGGGTAGGGCAGTTTGA
TCCTAAGACTGTGCGTATGGTCTTTGAGCTAAAACAAAACGTGAAGCCGCAGCTATTTGCGCTGGCGCCTGTGGCAGGCT
TTAAAGAACGTCTGGTGATGGATCTTTATCCTGCCAATGCGCAGGATATGCAGGATCCGCTGCTGGCGCTGCTGGAAGAC
TACAATAAAGGCGATCTGGATAAGCAGGTGCCGCCTTCGCAAAGCGGGCCGCAGCCCGGTAAAGCGGGGCGCGACCGCCC
CATTGTTATTATGCTTGATCCGGGACACGGCGGCGAAGACCCCGGCGCGATAGGCAAATACAAAACGCGTGAAAAAGACG
TGGTGCTCCAGATAGCCCGCCGCCTGCGCGCGTTGATCGAAAAAGAAGGCAACATGAAGGTCTATATGACGCGCAATGAA
GACATCTTCATTCCGTTAAAAGTCCGGGTGGCGAAAGCGCAGAAACAGCGCGCCGACCTGTTTGTCTCTATTCATGCGGA
TGCCTTCACCAGCCGGCAGCCTAGCGGTTCATCCGTATTCGCACTATCGACCAAAGGCGCCACCAGTACTGCGGCGAAAT
ATCTGGCGCAAACGCAGAACGCCTCGGACTTAATTGGCGGCGTGAGCAAAAGCGGTGACCGTTATGTCGACCATACGATG
TTCGATATGGTGCAATCGCTGACCATTGCCGATAGTTTGAAATTCGGTAAAGCGGTGTTGAAGCAGCTTGGTAAAATTAA
TGACCTGCATAAAAACAAGGTCGAGCAGGCGGGGTTTGCGGTGTTAAAGGCGCCCGATATTCCGTCTATTCTGGTCGAGA
CGGCGTTTATCAGTAACATTGAAGAAGAACGCAAGCTCAAGACCGCGACGTTCCAGCAACAGGTCGCAGAGTCGATTCTG
GCGGGCATTAAAGCGTATTTTGCCGATGGGGCGACGCTGGCGAGAAGAAGTTAA

Upstream 100 bases:

>100_bases
ACCCTTTTATAGATGACAGATTATGCGTCATTCGCTAAAGTTTCCGGTCAATTTGGATGAACTTTGTATGATCTCTATTT
AGTTTTTGCTCGGGAGAAGC

Downstream 100 bases:

>100_bases
CCTGAGATGACGGCGAGTTTATTGCGCGGGTAAGTAGGATGCTGCCCGCACTGTCGTTGCGGACCAGACGACAGATACAA
AAAAACACCCGTAAGGGTGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKYKQFALSNPDRVVVDIEDVNL
NSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
YNKGDLDKQVPPSQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTM
FDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFAVLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESIL
AGIKAYFADGATLARRS

Sequences:

>Translated_417_residues
MSGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKYKQFALSNPDRVVVDIEDVNL
NSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
YNKGDLDKQVPPSQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTM
FDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFAVLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESIL
AGIKAYFADGATLARRS
>Mature_416_residues
SGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKYKQFALSNPDRVVVDIEDVNLN
SVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDY
NKGDLDKQVPPSQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNED
IFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMF
DMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFAVLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESILA
GIKAYFADGATLARRS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=416, Percent_Identity=94.4711538461538, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=47.2340425531915, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=42.2222222222222, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45614; Mature: 45483

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKY
CCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCHHH
KQFALSNPDRVVVDIEDVNLNSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQN
HHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
VKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLDKQVPPSQSGPQPG
CCCEEEEECCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
CEEEEEEEEEHHHHHHCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCEE
VLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESILAGIKAYFADGATLARRS
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKY
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCHHH
KQFALSNPDRVVVDIEDVNLNSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQN
HHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
VKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLDKQVPPSQSGPQPG
CCCEEEEECCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
CEEEEEEEEEHHHHHHCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCEE
VLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESILAGIKAYFADGATLARRS
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]