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The map label for this gene is rumA [H]

Identifier: 16766263

GI number: 16766263

Start: 3105166

End: 3106461

Strand: Reverse

Name: rumA [H]

Synonym: STM2957

Alternate gene names: 16766263

Gene position: 3106461-3105166 (Counterclockwise)

Preceding gene: 16766265

Following gene: 16766262

Centisome position: 63.95

GC content: 54.4

Gene sequence:

>1296_bases
ATGGCGCAATTCTACTCTGCAAAACGGCGCGTGACGACGCGTCAGATCATAACCGTTAAAGTCAATGACCTCGATTCATT
TGGTCAGGGCGTAGCCCGTCATAACGGAAAAGCGCTGTTTATCCCCGGTTTATTGCCTGAGGAAAGCGCAGAAGTCATTA
TTACAGAAGATAAAAAGCAATTTGCCCGCGCACGCGTTTCGCGCCGTTTGAATGATAGCCCGGAACGAGAAACGCCGCGC
TGCCCGCATTTTGGCGTCTGCGGCGGTTGTCAGCAACAGCATGTCAGCATCGCCCTGCAACAACGCAGCAAAAGCGCGGC
GCTGGCGCGTTTGATGAAACACGAGGTGAATGACATCATCGCCGGCGCGCCTTGGGGTTATCGGCGTCGTGCGCGGTTAA
GTCTGAATTGCCCGCCGGATAAACCGCTGCAAATGGGATTTCGCAAAGCGGGTTCCAGCGATATCGTCAATGTCGAACAG
TGCCCCGTTTTGGCGCCCCAGCTTGCAGCATTGCTGCCCCGAATCAGGGCGTGTCTGGCGAGTTTACACGGCACCCGACA
TCTTGGGCATGTTGAGCTGGTACAGGCGGGAAGCGGTACGCTGATGATTCTGCGCCATACCGCGCCATTAAGTGCGGCGG
ATAAAGAAAAACTGGAACGCTTTTCGCATTCTGAGGGATTGTCTCTGTTTCTGGCGCCGTTTAGCGAGATACTGGAAACG
GTATCTGGCGAGGCGCCCTGGTATGATTCGCACGGCTTACGTTTAGCCTTCAGTCCGCGAGACTTTATTCAGGTTAATGA
GGCGGTAAACCAGCAAATGGTCGCCCGCGCGCTGGAGTGGCTTGATGTGCGCGCCGAGGATCGTGTCCTGGATCTGTTCT
GCGGAATGGGGAATTTTACGCTGCCGCTGGCAACGCGCGCGGCAAGCGTGATTGGGGTTGAGGGCGTTCCGGCGCTGGTA
GAAAAAGGCCGTGAAAACGCCATCCGCAATGGTTTACATAATGTGACATTCTTCCATGAGAACCTGGAGGAAGATGTCAC
GAAGCAGCCGTGGGCGAAAAACGGCTTTGACAAAGTCTTACTCGATCCTGCGCGTGCGGGGGCTACAGGAGTGATGCGAC
ATATTATAAAATTAAAACCTATTCGCATTGTTTATGTATCCTGTAACCCGGCGACGCTGGCGCGCGATAGTGAAGCGCTG
GTCAATGCGGGATATGAGGTTACGCGTTTAGCGATGCTCGACATGTTCCCGCACACAGGACATCTGGAATCAATGGTTCT
GTTCGAGCGCATGTAA

Upstream 100 bases:

>100_bases
GGATCAGAATCATCATGCGTGCGCGCAGGCTGTAGTTGGTCATGGAGTTCCGTTATGGGACAATTAGGGTCACACTGTTA
AATTGAGAAAAAGCTGGTCG

Downstream 100 bases:

>100_bases
TGATTACCGGCTTACCGACTTCGGTAGGCCTGGTCCCTTAAGGAGAGGACGATGGTCGCGGTAAGAAGTGCACATATTAA
TAAAGCTGGTGAATTTGATC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQFARARVSRRLNDSPERETPR
CPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDIIAGAPWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQ
CPVLAPQLAALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
VSGEAPWYDSHGLRLAFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVIGVEGVPALV
EKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEAL
VNAGYEVTRLAMLDMFPHTGHLESMVLFERM

Sequences:

>Translated_431_residues
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQFARARVSRRLNDSPERETPR
CPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDIIAGAPWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQ
CPVLAPQLAALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
VSGEAPWYDSHGLRLAFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVIGVEGVPALV
EKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEAL
VNAGYEVTRLAMLDMFPHTGHLESMVLFERM
>Mature_430_residues
AQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQFARARVSRRLNDSPERETPRC
PHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDIIAGAPWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQC
PVLAPQLAALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILETV
SGEAPWYDSHGLRLAFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVIGVEGVPALVE
KGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALV
NAGYEVTRLAMLDMFPHTGHLESMVLFERM

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=207, Percent_Identity=34.7826086956522, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI51173878, Length=207, Percent_Identity=34.7826086956522, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI269784680, Length=193, Percent_Identity=28.4974093264249, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI269784676, Length=193, Percent_Identity=28.4974093264249, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20149688, Length=193, Percent_Identity=28.4974093264249, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI269784678, Length=193, Percent_Identity=28.4974093264249, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=84.0277777777778, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787083, Length=373, Percent_Identity=27.3458445040214, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=34.0659340659341, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24666579, Length=215, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 47769; Mature: 47638

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQ
CCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHH
FARARVSRRLNDSPERETPRCPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAPWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQCPVLAPQLAALLPRIRACLA
CCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHH
SLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
VSGEAPWYDSHGLRLAFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFT
HCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
LPLATRAASVIGVEGVPALVEKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVL
EHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCEE
LDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALVNAGYEVTRLAMLDMFPHTG
ECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCCC
HLESMVLFERM
CHHHHHHHHCC
>Mature Secondary Structure 
AQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQ
CCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHH
FARARVSRRLNDSPERETPRCPHFGVCGGCQQQHVSIALQQRSKSAALARLMKHEVNDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAPWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQCPVLAPQLAALLPRIRACLA
CCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHH
SLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
VSGEAPWYDSHGLRLAFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFT
HCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
LPLATRAASVIGVEGVPALVEKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVL
EHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCEE
LDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALVNAGYEVTRLAMLDMFPHTG
ECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCCC
HLESMVLFERM
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA