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The map label for this gene is cysD [H]

Identifier: 16766241

GI number: 16766241

Start: 3074391

End: 3075299

Strand: Reverse

Name: cysD [H]

Synonym: STM2935

Alternate gene names: 16766241

Gene position: 3075299-3074391 (Counterclockwise)

Preceding gene: 16766243

Following gene: 16766240

Centisome position: 63.31

GC content: 53.03

Gene sequence:

>909_bases
ATGGATCAAAAACGACTCACCCACCTGCGGCAACTGGAGGCGGAAAGTATCCACATTATCCGTGAAGTGGCAGCGGAATT
CGCTAATCCGGTCATGCTGTACTCCATTGGTAAAGACTCCAGCGTCATGCTGCATCTGGCGCGTAAGGCGTTTTATCCGG
GTACGTTGCCGTTCCCGCTATTGCACGTTGATACCGGTTGGAAATTCCGTGAGATGTACGCCTTTCGCGATCGTACCGCC
AACGCATACGGCTGCGAATTGCTGGTACATAAAAATCCGGAAGGCGTGGCGATGGGCATCAATCCGTTCGTTCACGGTAG
CGCTAAACACACCGACATTATGAAAACCGAAGGGTTAAAGCAGGCGCTGAATAAGTACGGTTTTGATGCCGCGTTCGGCG
GCGCACGGCGCGATGAGGAAAAATCCCGTGCGAAAGAACGTATTTACTCCTTTCGTGACCGTTTCCATCGCTGGGACCCG
AAAAATCAGCGGCCAGAGTTGTGGCGTAACTATAACGGTCAGATTAATAAAGGCGAAAGTATACGCGTCTTCCCGCTCTC
CAACTGGACAGAGCAGGATATCTGGCAATACATCTGGCTGGAAAACATCGACATCGTGCCGCTGTATTTGGCTGCTGAAC
GCCCGGTTCTGGAACGTGACGGCATGTTGATGATGGTGGATGACGATCGTATCGATCTCCAGCCTGGCGAAGTCATCAAA
AAAAGGATGGTGCGCTTCCGTACACTTGGCTGTTGGCCGCTCACCGGGGCGGTGGAATCCCACGCGCAAACGCTGCCGGA
AATTATCGAAGAGATGCTGGTTTCTACCACCAGCGAGCGCCAGGGGAGGATGATTGACCGCGATCAGGCGGGGTCGATGG
AGCTTAAAAAACGTCAGGGGTATTTCTAA

Upstream 100 bases:

>100_bases
AACTACTCATTCCAATTCGTCATTTCATTCACTCTCCTGCGCTCCCTATAGTCGTGTAATCGAAATGTTTAGCAAAAAAC
GGTATTGCAAAGGAACGGTT

Downstream 100 bases:

>100_bases
GGAGCCGCCATGAACACCATACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATTGCCCAACAGCA
CAAAAGCCTGCTGCGTTTTT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDQKRLTHLRQLEAESIHIIREVAAEFANPVMLYSIGKDSSVMLHLARKAFYPGTLPFPLLHVDTGWKFREMYAFRDRTA
NAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWRNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMVDDDRIDLQPGEVIK
KRMVRFRTLGCWPLTGAVESHAQTLPEIIEEMLVSTTSERQGRMIDRDQAGSMELKKRQGYF

Sequences:

>Translated_302_residues
MDQKRLTHLRQLEAESIHIIREVAAEFANPVMLYSIGKDSSVMLHLARKAFYPGTLPFPLLHVDTGWKFREMYAFRDRTA
NAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWRNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMVDDDRIDLQPGEVIK
KRMVRFRTLGCWPLTGAVESHAQTLPEIIEEMLVSTTSERQGRMIDRDQAGSMELKKRQGYF
>Mature_302_residues
MDQKRLTHLRQLEAESIHIIREVAAEFANPVMLYSIGKDSSVMLHLARKAFYPGTLPFPLLHVDTGWKFREMYAFRDRTA
NAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
KNQRPELWRNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMVDDDRIDLQPGEVIK
KRMVRFRTLGCWPLTGAVESHAQTLPEIIEEMLVSTTSERQGRMIDRDQAGSMELKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=97.0198675496689, Blast_Score=615, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 35177; Mature: 35177

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKRLTHLRQLEAESIHIIREVAAEFANPVMLYSIGKDSSVMLHLARKAFYPGTLPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCE
LHVDTGWKFREMYAFRDRTANAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLK
EEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWRNYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCE
IRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMVDDDRIDLQPGEVIK
EEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHH
KRMVRFRTLGCWPLTGAVESHAQTLPEIIEEMLVSTTSERQGRMIDRDQAGSMELKKRQG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHCC
YF
CC
>Mature Secondary Structure
MDQKRLTHLRQLEAESIHIIREVAAEFANPVMLYSIGKDSSVMLHLARKAFYPGTLPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCE
LHVDTGWKFREMYAFRDRTANAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLK
EEECCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWRNYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCE
IRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMVDDDRIDLQPGEVIK
EEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHH
KRMVRFRTLGCWPLTGAVESHAQTLPEIIEEMLVSTTSERQGRMIDRDQAGSMELKKRQG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA