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The map label for this gene is phoB [H]

Identifier: 16763777

GI number: 16763777

Start: 450795

End: 451484

Strand: Direct

Name: phoB [H]

Synonym: STM0397

Alternate gene names: 16763777

Gene position: 450795-451484 (Clockwise)

Preceding gene: 16763773

Following gene: 16763778

Centisome position: 9.28

GC content: 54.64

Gene sequence:

>690_bases
ATGGCGAGACGTATTCTGGTCGTAGAAGATGAGGCGCCGATCCGTGAAATGGTGTGCTTCGTGCTCGAACAAAATGGCTT
TCAGCCCGTAGAAGCCGAAGATTATGACAGTGCGGTGAATAAACTTAATGAACCCTGGCCGGATCTCATCCTTCTTGACT
GGATGTTGCCCGGCGGGTCAGGGTTACAGTTTATTAAACATCTCAAACGTGAAGCCATGACCCGGGATATTCCTGTGGTT
ATGCTGACCGCAAGAGGTGAGGAAGAAGATCGTGTGCGCGGGCTGGAAACCGGCGCTGACGATTACATTACCAAGCCGTT
TTCCCCCAAGGAGTTGGTGGCGCGCATTAAAGCCGTTATGCGGCGCATCTCGCCGATGGCGGTCGAAGAGGTGATCGAGA
TGCAGGGACTGAGCCTTGATCCCGGCTCTCACCGCGTGATGACCGGCGACAGCCCGCTGGATATGGGGCCGACCGAATTT
AAATTACTCCATTTCTTTATGACGCACCCGGAACGGGTTTACAGTCGCGAGCAGCTACTCAATCATGTCTGGGGCACCAA
TGTGTACGTTGAAGACCGGACGGTCGACGTGCATATTCGCCGTTTGCGTAAGGCGCTGGAGCACAGCGGCCACGACCGGA
TGGTGCAAACGGTACGCGGGACAGGGTATCGTTTTTCGACCCGCTTTTAA

Upstream 100 bases:

>100_bases
ATCAACATGCTGTCAATGCCGCCTTTTTTTTTCATAAATCTGTCATAAATCTGACGCATAATGGCGCGGCATTGATAACT
AACGACTAACAGGGCAAATT

Downstream 100 bases:

>100_bases
TGCCTTTTCCGCCAGTAGAACACAGGTTTCTGGCGGAGAAGGCTTTCCATTCTTGAGGGAGTATGACGCGTGCTGGAACG
GCTGTCATGGAAAAGGCTGG

Product: transcriptional regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNKLNEPWPDLILLDWMLPGGSGLQFIKHLKREAMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPGSHRVMTGDSPLDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSTRF

Sequences:

>Translated_229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNKLNEPWPDLILLDWMLPGGSGLQFIKHLKREAMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPGSHRVMTGDSPLDMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSTRF
>Mature_228_residues
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNKLNEPWPDLILLDWMLPGGSGLQFIKHLKREAMTRDIPVVM
LTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPGSHRVMTGDSPLDMGPTEFK
LLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSTRF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=95.6331877729258, Blast_Score=452, Evalue=1e-129,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=41.0714285714286, Blast_Score=166, Evalue=8e-43,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.9912663755458, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=38.0090497737557, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.9775784753363, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=33.6363636363636, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=34.6320346320346, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=38.8646288209607, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=34.2222222222222, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=31.7391304347826, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=27.9661016949153, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=30.327868852459, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26404; Mature: 26273

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNKLNEPWPDLILLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GLQFIKHLKREAMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPGSHRVMTGDSPLDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNKLNEPWPDLILLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GLQFIKHLKREAMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIEMQGLSLDPGSHRVMTGDSPLDMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEHSGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]