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The map label for this gene is yajF [H]

Identifier: 16763773

GI number: 16763773

Start: 443966

End: 444874

Strand: Direct

Name: yajF [H]

Synonym: STM0393

Alternate gene names: 16763773

Gene position: 443966-444874 (Clockwise)

Preceding gene: 16763771

Following gene: 16763777

Centisome position: 9.14

GC content: 58.09

Gene sequence:

>909_bases
GTGCGTATTGGTATCGATTTAGGCGGCACAAAAACGGAAGTCATTGCGCTTGATGATGCAGGAGAGCAGCGGTTTCGCCA
TCGTCTGCCTACGCCGCGGGAGGATTATCAGCAAACGATAGAAACCATCGCTACGCTGGTCGACATGGCGGAACAGGCGA
CCGGCCAGACCGGTAGCGTCGGCATTGGCATTCCCGGTTCTCTCTCGCCTTACACCGGCGTGGTGAAAAACGCTAACTCT
ACGTGGCTTAACGGCCAGCCGTTCGATAGCGATGTGAGCCGTCGTCTGAAGCGTGAAGTGCGGTTAGCCAACGATGCCAA
TTGCCTGGCGGTATCAGAGGCGGTGGATGGCGCGGCGGCGGGGGCGCAAACGGTATTTGCGGTGATTATCGGTACCGGGT
GTGGCGCGGGCGTAGCGCTAAATGGCCGGGCGCACATCGGCGGCAACGGTACGGCAGGTGAATGGGGCCACAATCCGTTG
CCGTGGATGGATGACGATGAGTTGCGTTACCGCGAAGAAATTCCCTGTTATTGCGGTAAACAAGGCTGTATCGAAACCTT
TATTTCCGGTACGGGATTTGCCACGGATTACCAGCGTTTGAGCGGTAACGCGCTAAAAGGCGATGAGATTATTCGTCTGG
TCGACGCGCAGGACGCCGTGGCGGAACTGGCGTTAAGCCGTTATGAGCTGCGGCTGGCGAAAGCATTATCTCATGTCGTG
AATATTCTGGATCCGGATGTCATTGTGCTGGGCGGCGGTATGAGCAATGTTGAGCGCCTGTACAAGACGGTTCCGTCGCT
GATGAAATCGTTTGTTTTTGGCGGCGAGTGCGAGACGCCGGTACGTAAGGCGCGGCATGGCGATTCGAGCGGCGTGCGCG
GCGCGGCCTGGCTGTGGCCGCTGGCGTAA

Upstream 100 bases:

>100_bases
AATCCAGGCGGTATAGTAACGAATTGTCGGCAGGCTTGCATTGCCAATCAGAACGGTGAATTCTACCCTGTTGATATTCC
CAAAAAATGAGGAGCGGTAT

Downstream 100 bases:

>100_bases
CGTTGTTAGCCGGATGGCGCGTCATCCGGCAACCGGCGTGACCGATGGCTGCTTGTGTTTTAGACGTCCGTATACCAGCA
ACGACGACATGGCGGAAAAC

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSVGIGIPGSLSPYTGVVKNANS
TWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
PWMDDDELRYREEIPCYCGKQGCIETFISGTGFATDYQRLSGNALKGDEIIRLVDAQDAVAELALSRYELRLAKALSHVV
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWPLA

Sequences:

>Translated_302_residues
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSVGIGIPGSLSPYTGVVKNANS
TWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
PWMDDDELRYREEIPCYCGKQGCIETFISGTGFATDYQRLSGNALKGDEIIRLVDAQDAVAELALSRYELRLAKALSHVV
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWPLA
>Mature_302_residues
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSVGIGIPGSLSPYTGVVKNANS
TWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
PWMDDDELRYREEIPCYCGKQGCIETFISGTGFATDYQRLSGNALKGDEIIRLVDAQDAVAELALSRYELRLAKALSHVV
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWPLA

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=88.6666666666667, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1787363, Length=301, Percent_Identity=38.8704318936877, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786891, Length=272, Percent_Identity=23.1617647058824, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790522, Length=206, Percent_Identity=29.6116504854369, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32246; Mature: 32246

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
GIGIPGSLSPYTGVVKNANSTWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAA
EECCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDDDELRYREEIPCYCGK
HHHHEEHHEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYQRLSGNALKGDEIIRLVDAQDAVAELALSRYELRLAKALSHVV
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWP
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEECC
LA
CC
>Mature Secondary Structure
MRIGIDLGGTKTEVIALDDAGEQRFRHRLPTPREDYQQTIETIATLVDMAEQATGQTGSV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
GIGIPGSLSPYTGVVKNANSTWLNGQPFDSDVSRRLKREVRLANDANCLAVSEAVDGAAA
EECCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPLPWMDDDELRYREEIPCYCGK
HHHHEEHHEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYQRLSGNALKGDEIIRLVDAQDAVAELALSRYELRLAKALSHVV
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVERLYKTVPSLMKSFVFGGECETPVRKARHGDSSGVRGAAWLWP
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEECC
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]