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The map label for this gene is hemB [H]

Identifier: 218930775

GI number: 218930775

Start: 4235445

End: 4236467

Strand: Reverse

Name: hemB [H]

Synonym: YPO3771

Alternate gene names: 218930775

Gene position: 4236467-4235445 (Counterclockwise)

Preceding gene: 218930776

Following gene: 218930773

Centisome position: 91.03

GC content: 47.7

Gene sequence:

>1023_bases
ATGAGCTATGCATTTCCGGGCACCTTTCCTGGTCGCCGTATGCGCCGTGTACGCTGTCATGATTTCAGCCGCCGTTTGGT
TGCCGAGAATCACTTGACGGTCAATGACTTGATTTACCCGGTGTTTGTCATGGAAGGGACTCACCAGCAACAAGCCGTTT
CTTCAATGCCGGGGGTCTCTCGTATGACAATTGATCTGCTGCTGAAAGAAGCAGAAGCAATTGCTAAGCTCGGTGTGCCG
GTTATATCGCTATTCCCGGTCATTGAAGCAGGGAAAAAATCATTATATGCAGAAGAGGCATATAACCCGGATGGTTTAGT
TCAGCGTACTGTGCGTGCGTTGAAAGATGCAGTTCCTGAGCTAGGTATTCTGACTGACGTGGCACTCGATCCCTATACGA
CCCATGGTCAAGATGGTGTGATTGATTCTGATGGTTACGTGATCAATGATGTCACCAAAGAGATTCTAGTGCGTCAGGCA
CTATCGCATGCTGAAGCTGGCGCTGAGATTATTGCACCAAGCGATATGATGGATGGCCGTATAGGTGCAATTCGTGATCA
ATTAGAGCGCCAAGGGTTAGTGAACACCCAGATAATGGCTTATTCGGCTAAGTATGCGTCTTGCTACTATGGCCCATTCC
GCGATGCAATTGGCTCGAGCAGTAATCTAAAAGGGGGGGATAAAAAAACTTATCAGATGGATCCCGCCAACAGTGATGAA
GCACTGCAAGAAATAGCTCAAGATTTACAAGAAGGCGCTGATATGGTGATGGTAAAACCAGGAATGCCATATCTGGATGT
TGTACGCAGGGTGAAAGATACTTTTGGTGTTCCAACCTTTGCCTATCAGGTGTCTGGTGAATATGCCATGCATATGGCAG
CGATTCAGAATGGCTGGCTCCAAGAGAAACCGACGGTGATGGAGTCGTTGTTATGCTTTAAGCGTGCAGGTGCGGATGGC
GTATTAACTTATTTCGCAAAGCAAGTTGCGCAATGGCTACATGATGACCAGATGCAGCGCTAA

Upstream 100 bases:

>100_bases
AGGTTGCAGCCTGGCGACAAGAAGACCCTAATTGGCTAGGACAGAAAACTGATGAAAACGCCCGCCGGGTTTTCCGGCTG
GTTTGAGTTAGGAGAACAAT

Downstream 100 bases:

>100_bases
AGGATCATAACTAAGAGTCACAGCATTCAGATAGGCACTAACTGCGCCTATCTGAATGAATTCTATAATGATGAGGTAAT
ACTCATCCAGATTATTGTAA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSYAFPGTFPGRRMRRVRCHDFSRRLVAENHLTVNDLIYPVFVMEGTHQQQAVSSMPGVSRMTIDLLLKEAEAIAKLGVP
VISLFPVIEAGKKSLYAEEAYNPDGLVQRTVRALKDAVPELGILTDVALDPYTTHGQDGVIDSDGYVINDVTKEILVRQA
LSHAEAGAEIIAPSDMMDGRIGAIRDQLERQGLVNTQIMAYSAKYASCYYGPFRDAIGSSSNLKGGDKKTYQMDPANSDE
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWLQEKPTVMESLLCFKRAGADG
VLTYFAKQVAQWLHDDQMQR

Sequences:

>Translated_340_residues
MSYAFPGTFPGRRMRRVRCHDFSRRLVAENHLTVNDLIYPVFVMEGTHQQQAVSSMPGVSRMTIDLLLKEAEAIAKLGVP
VISLFPVIEAGKKSLYAEEAYNPDGLVQRTVRALKDAVPELGILTDVALDPYTTHGQDGVIDSDGYVINDVTKEILVRQA
LSHAEAGAEIIAPSDMMDGRIGAIRDQLERQGLVNTQIMAYSAKYASCYYGPFRDAIGSSSNLKGGDKKTYQMDPANSDE
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWLQEKPTVMESLLCFKRAGADG
VLTYFAKQVAQWLHDDQMQR
>Mature_339_residues
SYAFPGTFPGRRMRRVRCHDFSRRLVAENHLTVNDLIYPVFVMEGTHQQQAVSSMPGVSRMTIDLLLKEAEAIAKLGVPV
ISLFPVIEAGKKSLYAEEAYNPDGLVQRTVRALKDAVPELGILTDVALDPYTTHGQDGVIDSDGYVINDVTKEILVRQAL
SHAEAGAEIIAPSDMMDGRIGAIRDQLERQGLVNTQIMAYSAKYASCYYGPFRDAIGSSSNLKGGDKKTYQMDPANSDEA
LQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWLQEKPTVMESLLCFKRAGADGV
LTYFAKQVAQWLHDDQMQR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=39.7435897435897, Blast_Score=238, Evalue=4e-63,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=44.0625, Blast_Score=236, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321398, Length=321, Percent_Identity=35.8255451713396, Blast_Score=196, Evalue=6e-51,
Organism=Drosophila melanogaster, GI21358291, Length=292, Percent_Identity=38.6986301369863, Blast_Score=211, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37637; Mature: 37506

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAFPGTFPGRRMRRVRCHDFSRRLVAENHLTVNDLIYPVFVMEGTHQQQAVSSMPGVS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHHHHHCCCCH
RMTIDLLLKEAEAIAKLGVPVISLFPVIEAGKKSLYAEEAYNPDGLVQRTVRALKDAVPE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
LGILTDVALDPYTTHGQDGVIDSDGYVINDVTKEILVRQALSHAEAGAEIIAPSDMMDGR
CCHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEECCCHHCCCH
IGAIRDQLERQGLVNTQIMAYSAKYASCYYGPFRDAIGSSSNLKGGDKKTYQMDPANSDE
HHHHHHHHHHCCCCCHHHEEEECHHHHHCCCHHHHHCCCCCCCCCCCCCEEEECCCCCHH
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWL
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCC
QEKPTVMESLLCFKRAGADGVLTYFAKQVAQWLHDDQMQR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SYAFPGTFPGRRMRRVRCHDFSRRLVAENHLTVNDLIYPVFVMEGTHQQQAVSSMPGVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHHHHHCCCCH
RMTIDLLLKEAEAIAKLGVPVISLFPVIEAGKKSLYAEEAYNPDGLVQRTVRALKDAVPE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
LGILTDVALDPYTTHGQDGVIDSDGYVINDVTKEILVRQALSHAEAGAEIIAPSDMMDGR
CCHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEECCCHHCCCH
IGAIRDQLERQGLVNTQIMAYSAKYASCYYGPFRDAIGSSSNLKGGDKKTYQMDPANSDE
HHHHHHHHHHCCCCCHHHEEEECHHHHHCCCHHHHHCCCCCCCCCCCCCEEEECCCCCHH
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWL
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCC
QEKPTVMESLLCFKRAGADGVLTYFAKQVAQWLHDDQMQR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]