Click here to switch to the map view.

The map label for this gene is fre [H]

Identifier: 218930772

GI number: 218930772

Start: 4232347

End: 4233048

Strand: Reverse

Name: fre [H]

Synonym: YPO3768

Alternate gene names: 218930772

Gene position: 4233048-4232347 (Counterclockwise)

Preceding gene: 218930773

Following gene: 218930769

Centisome position: 90.96

GC content: 48.72

Gene sequence:

>702_bases
ATGACAACTTTAAGCTGTAAAGTAACCTCCGTAGAGGCCATTACCGATACGGTGTACCGGGTGCAATTGGTACCTGAATC
TTCTTTTTCTTTCCGCGCCGGGCAGTATTTGATGGTAGTGATGGATGAGCGTGATAAGCGCCCATTCTCGATGGCTTCCA
CACCGTTTCAGCAGGACTTTATCGAGTTACATATCGGTGCTTCGGAGCTGAATTTGTACGCAATGGCAGTGATGGATCGC
ATCCTAAAAGAGAAAAAACTGGATGTTGATATTCCTCATGGAGATGCCTGGTTCCGTGAAGGGAGCAAGCGTCCGTTGGT
TCTGATTGCTGGCGGTACTGGCTTCTCTTATGCGCGATCCATCTTGTTAGCCGCGTTAGCGGAACAACCTGATCGCGAAG
TCTCTATCTATTGGGGCGGGCGCGAAGCGGTACACCTGTATGATTTAGGTGAGTTGGAAGCACTATCGATTAAGTATCCG
CAACTGAAAGTCATTCCCGTAGTAGAACAACCGGAAGAGGGTTGGCGTGGGCGCACAGGGACTGTACTGAGTGCTGTACT
ACAAGATTACGGTTCTTTAGCTGAGCAGGATATTTACATCGCGGGGCGTTTTGAAATGGCTAAAATCGCCCGTGAGCGTT
TTTGTGTGGAGCGTGGCGCACAGGAAGAACATATCTTTGGTGATGCTTTTGCCTTTATCTAA

Upstream 100 bases:

>100_bases
GTGGGATGAGCTGGCCATTTTCAGTGACAAAGACGCGAAACGCTAACTAGCGTCGGCTTTGTACTTGGTTTTGCATTTGA
TGACCCGACAGAGGGAAGGC

Downstream 100 bases:

>100_bases
AAGCGATAAAAAGCCCGCCTCTGACCGGCGGGGAAATAGCAACTAAACACGATATACCTCAGTTATCAGGGCCTACGTTC
AGGCCCCTAATTTCTTGATG

Product: FMN reductase

Products: NA

Alternate protein names: Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase C; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTTLSCKVTSVEAITDTVYRVQLVPESSFSFRAGQYLMVVMDERDKRPFSMASTPFQQDFIELHIGASELNLYAMAVMDR
ILKEKKLDVDIPHGDAWFREGSKRPLVLIAGGTGFSYARSILLAALAEQPDREVSIYWGGREAVHLYDLGELEALSIKYP
QLKVIPVVEQPEEGWRGRTGTVLSAVLQDYGSLAEQDIYIAGRFEMAKIARERFCVERGAQEEHIFGDAFAFI

Sequences:

>Translated_233_residues
MTTLSCKVTSVEAITDTVYRVQLVPESSFSFRAGQYLMVVMDERDKRPFSMASTPFQQDFIELHIGASELNLYAMAVMDR
ILKEKKLDVDIPHGDAWFREGSKRPLVLIAGGTGFSYARSILLAALAEQPDREVSIYWGGREAVHLYDLGELEALSIKYP
QLKVIPVVEQPEEGWRGRTGTVLSAVLQDYGSLAEQDIYIAGRFEMAKIARERFCVERGAQEEHIFGDAFAFI
>Mature_232_residues
TTLSCKVTSVEAITDTVYRVQLVPESSFSFRAGQYLMVVMDERDKRPFSMASTPFQQDFIELHIGASELNLYAMAVMDRI
LKEKKLDVDIPHGDAWFREGSKRPLVLIAGGTGFSYARSILLAALAEQPDREVSIYWGGREAVHLYDLGELEALSIKYPQ
LKVIPVVEQPEEGWRGRTGTVLSAVLQDYGSLAEQDIYIAGRFEMAKIARERFCVERGAQEEHIFGDAFAFI

Specific function: Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduces the complexed Fe(3+) iron of siderophores to Fe(2+), thus releasing it from the chelator [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=233, Percent_Identity=77.6824034334764, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: =1.16.1.3; =1.5.1.29 [H]

Molecular weight: Translated: 26222; Mature: 26091

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLSCKVTSVEAITDTVYRVQLVPESSFSFRAGQYLMVVMDERDKRPFSMASTPFQQDF
CCEEEEEEEEHHHHHCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCC
IELHIGASELNLYAMAVMDRILKEKKLDVDIPHGDAWFREGSKRPLVLIAGGTGFSYARS
EEEEECHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCEEEEECCCCHHHHHH
ILLAALAEQPDREVSIYWGGREAVHLYDLGELEALSIKYPQLKVIPVVEQPEEGWRGRTG
HHHHHHHCCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCEEEEEEEECCCCCCCCCCH
TVLSAVLQDYGSLAEQDIYIAGRFEMAKIARERFCVERGAQEEHIFGDAFAFI
HHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCHHEECHHHHCC
>Mature Secondary Structure 
TTLSCKVTSVEAITDTVYRVQLVPESSFSFRAGQYLMVVMDERDKRPFSMASTPFQQDF
CEEEEEEEEHHHHHCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCC
IELHIGASELNLYAMAVMDRILKEKKLDVDIPHGDAWFREGSKRPLVLIAGGTGFSYARS
EEEEECHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCEEEEECCCCHHHHHH
ILLAALAEQPDREVSIYWGGREAVHLYDLGELEALSIKYPQLKVIPVVEQPEEGWRGRTG
HHHHHHHCCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCEEEEEEEECCCCCCCCCCH
TVLSAVLQDYGSLAEQDIYIAGRFEMAKIARERFCVERGAQEEHIFGDAFAFI
HHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCHHEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]