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The map label for this gene is thiF [H]

Identifier: 218930746

GI number: 218930746

Start: 4193298

End: 4194095

Strand: Direct

Name: thiF [H]

Synonym: YPO3741

Alternate gene names: 218930746

Gene position: 4193298-4194095 (Clockwise)

Preceding gene: 218930745

Following gene: 218930747

Centisome position: 90.11

GC content: 54.01

Gene sequence:

>798_bases
ATGAAAAATGACCAGACGCTAAGTGACAGTGAATTTTTACGCTATAGCCGCCAACTGCTACTGGAAGATATCGGCCCGGA
AGGGCAACGAAAGCTAAAGGGTGCCAGTGTGCTGATTGTTGGCCTCGGTGGGCTGGGCTCCCCCGCAGCGCTCTATCTGG
CCGCCGCCGGTGTCGGTCAGTTACTGCTGGCAGACGATGACCAGTTGGATCTCACTAATTTGCAGCGCCAGATTCTGTAT
CGTACAGCAGATATCGGCCAAACGTTATCGAACCCCACCTCATCGGTTAGCCACAACAAGGCCCGGTTAGCGCAACGCCA
TTTGCAGCAACTCAATCCACAGATCCGCATTGTCGCACTGGATACACGGCTGACAGGACAAGTATTGACCGATGCTGTCG
CTAATGCAGATTTAGTACTGGATTGCAGTGACAATATGGAGACACGCCATCAGGTGAATGCCGCCTGTGTCACGGCACAA
AAACCACTAATCAGCGGCAGTGCTGTGGGGTTCAGTGGACAACTGTTGGTGATTGAACCGCCCTATGCCCAAGGCTGCTA
CGCCTGTATTTATCCAGATAAAGAGTTGCCTCAACGTAACTGCCGTACCGCGGGTGTTCTGGGGCCGGTGGTCGGTGTCA
TCGGTACCTTGCAGGCGCTGGAAGCCATTAAAATGCTCGCGGGAATACCATTAGCACTGAGCGGTAAATTGCGGCTATTT
GATGGCAAACAGCAGAGTTGGAGCACCCTGCAACTGACAAGGGCAGCCCATTGCCCGGCGTGTGGAGGTGGGTTGTGA

Upstream 100 bases:

>100_bases
GTCGGCAGTGTCGCCGTGGTCAGCGCCATTACGTTGGCAAGTGACTGGCAACGGGCAACCGCACAATTACTGCACCTGAT
CGAGGGTAAGGAGTTGGCTG

Downstream 100 bases:

>100_bases
AGACCAATTACATACCAATCATGCTCAATGACCAGCCATTGGAGGTGGAGTGCAATCTCACCGCCGAGATGCTGCTTAAT
CAACTTAAACACCACCAGCC

Product: thiamine biosynthesis protein ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKNDQTLSDSEFLRYSRQLLLEDIGPEGQRKLKGASVLIVGLGGLGSPAALYLAAAGVGQLLLADDDQLDLTNLQRQILY
RTADIGQTLSNPTSSVSHNKARLAQRHLQQLNPQIRIVALDTRLTGQVLTDAVANADLVLDCSDNMETRHQVNAACVTAQ
KPLISGSAVGFSGQLLVIEPPYAQGCYACIYPDKELPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGIPLALSGKLRLF
DGKQQSWSTLQLTRAAHCPACGGGL

Sequences:

>Translated_265_residues
MKNDQTLSDSEFLRYSRQLLLEDIGPEGQRKLKGASVLIVGLGGLGSPAALYLAAAGVGQLLLADDDQLDLTNLQRQILY
RTADIGQTLSNPTSSVSHNKARLAQRHLQQLNPQIRIVALDTRLTGQVLTDAVANADLVLDCSDNMETRHQVNAACVTAQ
KPLISGSAVGFSGQLLVIEPPYAQGCYACIYPDKELPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGIPLALSGKLRLF
DGKQQSWSTLQLTRAAHCPACGGGL
>Mature_265_residues
MKNDQTLSDSEFLRYSRQLLLEDIGPEGQRKLKGASVLIVGLGGLGSPAALYLAAAGVGQLLLADDDQLDLTNLQRQILY
RTADIGQTLSNPTSSVSHNKARLAQRHLQQLNPQIRIVALDTRLTGQVLTDAVANADLVLDCSDNMETRHQVNAACVTAQ
KPLISGSAVGFSGQLLVIEPPYAQGCYACIYPDKELPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGIPLALSGKLRLF
DGKQQSWSTLQLTRAAHCPACGGGL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=260, Percent_Identity=45.7692307692308, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4885649, Length=157, Percent_Identity=30.5732484076433, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI23510338, Length=195, Percent_Identity=26.1538461538462, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI23510340, Length=195, Percent_Identity=26.1538461538462, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI87082356, Length=257, Percent_Identity=61.0894941634241, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI1787048, Length=256, Percent_Identity=42.96875, Blast_Score=197, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17540406, Length=268, Percent_Identity=39.1791044776119, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI193203301, Length=173, Percent_Identity=30.635838150289, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321903, Length=266, Percent_Identity=36.0902255639098, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322639, Length=197, Percent_Identity=27.4111675126904, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320598, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582879, Length=261, Percent_Identity=43.2950191570881, Blast_Score=190, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24660640, Length=171, Percent_Identity=32.1637426900585, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28573937, Length=194, Percent_Identity=26.8041237113402, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 28193; Mature: 28193

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNDQTLSDSEFLRYSRQLLLEDIGPEGQRKLKGASVLIVGLGGLGSPAALYLAAAGVGQ
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCE
LLLADDDQLDLTNLQRQILYRTADIGQTLSNPTSSVSHNKARLAQRHLQQLNPQIRIVAL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
DTRLTGQVLTDAVANADLVLDCSDNMETRHQVNAACVTAQKPLISGSAVGFSGQLLVIEP
CCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEC
PYAQGCYACIYPDKELPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGIPLALSGKLRLF
CCCCCCEEEECCCHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEE
DGKQQSWSTLQLTRAAHCPACGGGL
CCCCCCCHHEEEHHHCCCCCCCCCC
>Mature Secondary Structure
MKNDQTLSDSEFLRYSRQLLLEDIGPEGQRKLKGASVLIVGLGGLGSPAALYLAAAGVGQ
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCE
LLLADDDQLDLTNLQRQILYRTADIGQTLSNPTSSVSHNKARLAQRHLQQLNPQIRIVAL
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
DTRLTGQVLTDAVANADLVLDCSDNMETRHQVNAACVTAQKPLISGSAVGFSGQLLVIEP
CCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEC
PYAQGCYACIYPDKELPQRNCRTAGVLGPVVGVIGTLQALEAIKMLAGIPLALSGKLRLF
CCCCCCEEEECCCHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEE
DGKQQSWSTLQLTRAAHCPACGGGL
CCCCCCCHHEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]