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The map label for this gene is thiE [H]

Identifier: 218930745

GI number: 218930745

Start: 4192661

End: 4193308

Strand: Direct

Name: thiE [H]

Synonym: YPO3740

Alternate gene names: 218930745

Gene position: 4192661-4193308 (Clockwise)

Preceding gene: 218930744

Following gene: 218930746

Centisome position: 90.09

GC content: 53.4

Gene sequence:

>648_bases
GTGGCTACACCAGGTTTCCCCTCCACGGAACAGCGTCTTGGGCTCTACCCAGTGGTGGACTCATTATTATGGATAGAACG
CCTGCTGGCAGCCGGAGTCACGACGCTACAACTGCGCATTAAAAATGCAGACGATGCTCAGGTGGAGCAAGATATTGTCG
CGGCCATTGAATTAGGAAAACGCTATCAGGCGCGCTTATTTATCAATGATTACTGGCAGTTGGCGGTTAAACACGGCGCT
TATGGCGTTCATCTCGGTCAGGAAGATCTGGAAGCCGCTGATTTAGCGGCAATTCAGCAAGCGGGGTTGCGGCTCGGCAT
TTCTACCCATGATGAACATGAGTTGGCGGTGGCAAAAACACTGCGCCCCTCTTATATCGCACTGGGGCATATTTTTCCCA
CTCAGACCAAACAGATGCCGTCATCCCCACAGGGGTTGGCGTCGTTAAGCCGTCAGGTGAAGAATACGCCGGATTACCCA
ACTGTGGCTATCGGTGGCATCAGTATTGAGCGGGTGCCACACGTATTAGCAACCGGCGTCGGCAGTGTCGCCGTGGTCAG
CGCCATTACGTTGGCAAGTGACTGGCAACGGGCAACCGCACAATTACTGCACCTGATCGAGGGTAAGGAGTTGGCTGATG
AAAAATGA

Upstream 100 bases:

>100_bases
ATGTCGGCCGAGTTTCGCTCCCGTGGCAGTGAGCTATACCACCGCCCGGCGAATCTGAGTGCGGAGGCCAATAATGAGCC
AACTTGATGCCCTCTCTCCT

Downstream 100 bases:

>100_bases
CCAGACGCTAAGTGACAGTGAATTTTTACGCTATAGCCGCCAACTGCTACTGGAAGATATCGGCCCGGAAGGGCAACGAA
AGCTAAAGGGTGCCAGTGTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYWQLAVKHGA
YGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYP
TVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Sequences:

>Translated_215_residues
MATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYWQLAVKHGA
YGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYP
TVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
>Mature_214_residues
ATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYWQLAVKHGAY
GVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPT
VAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=70.7317073170732, Blast_Score=295, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6325042, Length=228, Percent_Identity=28.0701754385965, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23275; Mature: 23144

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGK
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
RYQARLFINDYWQLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKT
HHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
LRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIERVPHVLATGV
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCC
GSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGK
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
RYQARLFINDYWQLAVKHGAYGVHLGQEDLEAADLAAIQQAGLRLGISTHDEHELAVAKT
HHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
LRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIERVPHVLATGV
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCC
GSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA