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The map label for this gene is purH [H]

Identifier: 218930733

GI number: 218930733

Start: 4181118

End: 4182707

Strand: Direct

Name: purH [H]

Synonym: YPO3728

Alternate gene names: 218930733

Gene position: 4181118-4182707 (Clockwise)

Preceding gene: 218930727

Following gene: 218930734

Centisome position: 89.84

GC content: 51.38

Gene sequence:

>1590_bases
ATGCAACAACGCCGTCCAATCCGCCGTGCTCTACTCAGTGTGTCTGACAAAGCAGGTATCATCGAATTCGCCCAAGCACT
TTCTCAACGCGGTATCGAGTTACTTTCCACCGGTGGGACTGCCCGCCTGCTGGCTGATGCTGGTTTACCCGTTACCGAAG
TGTCTGACTACACCGGTTTCCCGGAAATGATGGATGGACGTGTGAAGACTTTGCATCCAAAGGTGCATGGTGGGATTTTA
GGTCGTCGTGGTCAGGATGATGGCATCATGGCTCAACATGGCATTCAGCCCATTGATATTGTCGTCGTTAATTTATATCC
CTTCGCCCAGACGGTTGCCCGCCCGGATTGCTCGTTGGAAGATGCGGTTGAGAATATTGATATTGGTGGCCCAACCATGG
TTCGCTCTGCGGCCAAGAACCATAAAGATGTCGCCATCGTGGTGAAGAGTAGCGACTACCCCGCCATTATTACTGAGCTT
GATAATAATGATGGTTCGTTGACTTACCCCACCCGCTTCAATCTGGCCATTAAAGCTTTCGAACACACCGCCGCCTACGA
CAGCATGATCGCCAACTACTTCGGCACACTGGTGCCACCTTATCATGGTGATACGGAACAGCCTTCCGGCCACTTCCCTC
GCACCCTAAATCTTAACTATATAAAGAAGCAGGATATGCGTTACGGTGAAAACAGCCACCAGCAAGCTGCCTTCTATATA
GAAGAAGATGTCAAAGAGGCATCCGTTGCCACTGCCCAGCAATTACAAGGGAAAGCCCTCTCTTATAACAATATTGCGGA
TACCGATGCCGCGCTGGAATGCGTGAAAGAGTTCAGTGAACCAGCCTGTGTGATCGTTAAACATGCCAACCCATGCGGTG
TGGCTATCGGTGATTCTATTCTTGCCGCTTATGAACGTGCCTATCAAACCGATCCAACCTCAGCTTTCGGTGGCATCATC
GCCTTTAACCGTGAATTGGATGCAGCAACGGCCAGCGCGATCATCAGCCGCCAGTTTGTCGAAGTGATCATTGCGCCAAC
AGTCAGCTCTGATGCATTGGCATTGCTTGCAGCTAAACAAAATGTCCGAGTCCTGACTTGTGGCCAGTGGCAAGCACGTT
CAGCAGGTTTAGATTTCAAACGTGTTAATGGGGGTTTGCTGGTACAAGAACGCGATTTAGGTATGGTGACGGCGGCCGAC
CTTCGCGTGGTTTCCAAGCGTCAGCCTACCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAAGTGGCTAAGTTTGT
TAAATCCAATGCGATTGTCTATGCCCGCGATAACATGACAATCGGTATAGGTGCCGGCCAAATGAGCCGCGTGTACTCTG
CGAAAATAGCCGGTATCAAGGCCGCAGATGAAGGGCTGGAAGTGGCTGGCTCAGCCATGGCCTCTGATGCCTTCTTCCCG
TTCCGTGATGGTATTGATGCCGCCGCGGCTGTGGGCATTACTTGTGTCATCCAACCGGGCGGCTCAATTCGTGATGATGA
AGTCATCGCGGCTGCTGATGAACACAGTATTGCCATGATCTTCACCGACATGCGCCATTTCCGTCATTAA

Upstream 100 bases:

>100_bases
ATATCAACGAAATAGTCTGTAGCGCCCTATTAATTGATAGTGAAGAACTCTGTAATTGATAATGAAGAAAACTGCGACTC
CAAATCCAAGGGATAAAACC

Downstream 100 bases:

>100_bases
TTAACCCTCTTATATAGGAAGGGGCGCTTGATGATGTGCCCCTTCACGGAGTCATCCAGATGAATATTTTGATAATTGGT
AACGGCGGTCGTGAACACGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITEL
DNNDGSLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQDMRYGENSHQQAAFYI
EEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSILAAYERAYQTDPTSAFGGII
AFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAAD
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSAMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH

Sequences:

>Translated_529_residues
MQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITEL
DNNDGSLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQDMRYGENSHQQAAFYI
EEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSILAAYERAYQTDPTSAFGGII
AFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAAD
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSAMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH
>Mature_529_residues
MQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITEL
DNNDGSLTYPTRFNLAIKAFEHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQDMRYGENSHQQAAFYI
EEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSILAAYERAYQTDPTSAFGGII
AFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQNVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAAD
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSAMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=486, Percent_Identity=38.6831275720165, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=87.1455576559546, Blast_Score=961, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=620, Percent_Identity=34.6774193548387, Blast_Score=294, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI71985574, Length=358, Percent_Identity=28.4916201117318, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=36.5217391304348, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=36.0082304526749, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6323056, Length=623, Percent_Identity=31.3001605136437, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24649832, Length=612, Percent_Identity=35.7843137254902, Blast_Score=308, Evalue=8e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57154; Mature: 57154

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCEEEEEEEEEEHHHHHHCCCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITELDNNDGSLTYPTRFNLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCEECCEEECEEEEEH
EHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCCEEEEE
EEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSI
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
LAAYERAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECC
NVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAADLRVVSKRQPTEQELRDALFC
CCEEEEECCCCCCCCCCCEEECCCCEEEEECCCCEEEHHHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSAMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCHHHHHHHHHCCCCCC
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC
>Mature Secondary Structure
MQQRRPIRRALLSVSDKAGIIEFAQALSQRGIELLSTGGTARLLADAGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDGIMAQHGIQPIDIVVVNLYPFAQTVARPDCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCEEEEEEEEEEHHHHHHCCCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYPAIITELDNNDGSLTYPTRFNLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCEECCEEECEEEEEH
EHTAAYDSMIANYFGTLVPPYHGDTEQPSGHFPRTLNLNYIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCCEEEEE
EEDVKEASVATAQQLQGKALSYNNIADTDAALECVKEFSEPACVIVKHANPCGVAIGDSI
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
LAAYERAYQTDPTSAFGGIIAFNRELDAATASAIISRQFVEVIIAPTVSSDALALLAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECC
NVRVLTCGQWQARSAGLDFKRVNGGLLVQERDLGMVTAADLRVVSKRQPTEQELRDALFC
CCEEEEECCCCCCCCCCCEEECCCCEEEEECCCCEEEHHHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYARDNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVAGSAMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCHHHHHHHHHCCCCCC
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHSIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA